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- W2008749062 abstract "Twenty-six experimentally infected calves were monitored daily for oocyst excretion. All began excreting oocysts 3–6 days p.i. Most calves (n=23) excreted oocysts for 6–9 days, with a daily range from 4 × 102 to 4.15 × 107 oocystsg−1 of faeces. Over half the calves excreted peak numbers of oocysts 6–8 days p.i. Diarrhoea, observed intermittently beginning as early as day 3 p.i., lasted 4–16 days and varied greatly in severity from calf to calf. In a second study, nine of 18 calves were orally inoculated with 5 × 106 oocysts between birth and 2 days of age and nine remained uninfected. Monoclonal antibodies for cell surface markers indicated substantial increases in CD4 + and CD8 + T cells in the intraepithelial lymphocyte population of the ilea of infected calves at 7–9 days of age. RT-PCR demonstrated increases in mRNA for interleukin-12 and interferon-γ that correlated with increases in both CD4 + and CD8 + intraepithelial lymphocyte cells. Increased mRNA for interleukin-12 and interferon-γ from lamina propria lymphocytes correlated with increased numbers of CD8 + cells. No changes were found in interleukin-2, interleukin-4 or interleukin-10 mRNA levels. However, interleukin-15 mRNA, possibly from epithelial cells contaminating intraepithelial lymphocytes, was decreased in infected calves and had a negative correlation with increases in CD4 + and CD8 + cells. No differences were detected in mRNA levels for cytokines from lymph node lymphocytes." @default.
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- W2008749062 date "1998-01-01" @default.
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- W2008749062 title "Cryptosporidium parvum infection in bovine neonates: dynamic clinical, parasitic and immunologic patterns" @default.
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- W2008749062 doi "https://doi.org/10.1016/s0020-7519(97)00170-7" @default.
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