Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008762908> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2008762908 endingPage "9a" @default.
- W2008762908 startingPage "9a" @default.
- W2008762908 abstract "Neurofilaments (NFs) - the major cytoskeletal constituent of myelinated axons in vertebrates - consist of three molecular-weight subunit proteins NF-L (low), NF-M (medium), and NF-H (high), assembled to form mature filaments with protruding unstructured C-terminus sidearms. Liquid crystal gel networks of sidearm-mediated NF assemblies play a key role in the mechanical stability of neuronal processes. Disruptions of the NF-network, due to NF over-accumulation or incorrect sidearm interactions, is a hallmark of motor neuron diseases including amyotrophic lateral sclerosis. Using synchrotron x-ray scattering [1,2], and various microscopy techniques [1,3] we report on the role of the subunit sidearms on the structure and interaction of NF. We will show a direct measurement of forces in reconstituted NF-gels under osmotic pressure (P). With increasing pressure near physiological salt, NF-LMH, comprised of the three subunits near in-vivo composition, or NF-LH gels, undergo for P>Pc ≈ 10 kPa, an abrupt nonreversible gel expanded to gel condensed transition. The transition indicates sidearm-mediated attractions between NFs consistent with an electrostatic model of interpenetrating chains. In contrast, NF-LM gels, remain in a collapsed state for PPc. In addition, single filament AFM measurements show that bending modulus is also regulated via intra-filaments interactions [4]. These findings, which delineate the distinct roles of NF-M and NF-H in regulating neurofilament interactions, shed light on possible mechanisms for disruptions of optimal mechanical network properties. Supported by DOE DE-FG-02-06ER46314, NSF DMR-0803103, and the Human Frontier Science Program organization. [1] J.B. Jones, C.R. Safinya, Biophys. J. 95, 823 (2008); [2] R. Beck et al., Nature Mat. (2009) in press; [3] H. Hess et al. Langmuir 24, 8397 (2008) [4] R. Beck et al. to be published." @default.
- W2008762908 created "2016-06-24" @default.
- W2008762908 creator A5008710205 @default.
- W2008762908 creator A5015488088 @default.
- W2008762908 creator A5024454957 @default.
- W2008762908 creator A5072682355 @default.
- W2008762908 creator A5082765578 @default.
- W2008762908 creator A5087083984 @default.
- W2008762908 creator A5088236477 @default.
- W2008762908 date "2010-01-01" @default.
- W2008762908 modified "2023-09-30" @default.
- W2008762908 title "Structures and Interactions in Neurofilament: Gel Expanded To Gel Condensed Transition" @default.
- W2008762908 doi "https://doi.org/10.1016/j.bpj.2009.12.056" @default.
- W2008762908 hasPublicationYear "2010" @default.
- W2008762908 type Work @default.
- W2008762908 sameAs 2008762908 @default.
- W2008762908 citedByCount "1" @default.
- W2008762908 countsByYear W20087629082012 @default.
- W2008762908 crossrefType "journal-article" @default.
- W2008762908 hasAuthorship W2008762908A5008710205 @default.
- W2008762908 hasAuthorship W2008762908A5015488088 @default.
- W2008762908 hasAuthorship W2008762908A5024454957 @default.
- W2008762908 hasAuthorship W2008762908A5072682355 @default.
- W2008762908 hasAuthorship W2008762908A5082765578 @default.
- W2008762908 hasAuthorship W2008762908A5087083984 @default.
- W2008762908 hasAuthorship W2008762908A5088236477 @default.
- W2008762908 hasBestOaLocation W20087629081 @default.
- W2008762908 hasConcept C104292427 @default.
- W2008762908 hasConcept C104317684 @default.
- W2008762908 hasConcept C12554922 @default.
- W2008762908 hasConcept C14228908 @default.
- W2008762908 hasConcept C142669718 @default.
- W2008762908 hasConcept C1491633281 @default.
- W2008762908 hasConcept C157207234 @default.
- W2008762908 hasConcept C171250308 @default.
- W2008762908 hasConcept C185592680 @default.
- W2008762908 hasConcept C192562407 @default.
- W2008762908 hasConcept C203014093 @default.
- W2008762908 hasConcept C204232928 @default.
- W2008762908 hasConcept C55493867 @default.
- W2008762908 hasConcept C78383274 @default.
- W2008762908 hasConcept C8010536 @default.
- W2008762908 hasConcept C86803240 @default.
- W2008762908 hasConcept C9603476 @default.
- W2008762908 hasConceptScore W2008762908C104292427 @default.
- W2008762908 hasConceptScore W2008762908C104317684 @default.
- W2008762908 hasConceptScore W2008762908C12554922 @default.
- W2008762908 hasConceptScore W2008762908C14228908 @default.
- W2008762908 hasConceptScore W2008762908C142669718 @default.
- W2008762908 hasConceptScore W2008762908C1491633281 @default.
- W2008762908 hasConceptScore W2008762908C157207234 @default.
- W2008762908 hasConceptScore W2008762908C171250308 @default.
- W2008762908 hasConceptScore W2008762908C185592680 @default.
- W2008762908 hasConceptScore W2008762908C192562407 @default.
- W2008762908 hasConceptScore W2008762908C203014093 @default.
- W2008762908 hasConceptScore W2008762908C204232928 @default.
- W2008762908 hasConceptScore W2008762908C55493867 @default.
- W2008762908 hasConceptScore W2008762908C78383274 @default.
- W2008762908 hasConceptScore W2008762908C8010536 @default.
- W2008762908 hasConceptScore W2008762908C86803240 @default.
- W2008762908 hasConceptScore W2008762908C9603476 @default.
- W2008762908 hasIssue "3" @default.
- W2008762908 hasLocation W20087629081 @default.
- W2008762908 hasOpenAccess W2008762908 @default.
- W2008762908 hasPrimaryLocation W20087629081 @default.
- W2008762908 hasRelatedWork W1543771029 @default.
- W2008762908 hasRelatedWork W1984587337 @default.
- W2008762908 hasRelatedWork W1986686298 @default.
- W2008762908 hasRelatedWork W2004086215 @default.
- W2008762908 hasRelatedWork W2008762908 @default.
- W2008762908 hasRelatedWork W2010495967 @default.
- W2008762908 hasRelatedWork W2022122163 @default.
- W2008762908 hasRelatedWork W2036377561 @default.
- W2008762908 hasRelatedWork W2059652918 @default.
- W2008762908 hasRelatedWork W2082779460 @default.
- W2008762908 hasVolume "98" @default.
- W2008762908 isParatext "false" @default.
- W2008762908 isRetracted "false" @default.
- W2008762908 magId "2008762908" @default.
- W2008762908 workType "article" @default.