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- W2008770982 abstract "Two triple-stranded helices, Poly(G)·Poly(C)·Poly(C+) I and Poly(G)·Poly(G)·Poly(C) II, were prepared from 1 : 2 and 2 : 1 mixtures of Poly(G) and Poly(C) in 0.4 M NaCl–acetate buffer at pH 5.5 and at 40°C. Only one triple helix, Poly(A)·Poly(U)·Poly(U) III, could be prepared from the mixture of Poly(A) and Poly(U) in 0.225 M NaCl–phosphate buffer at pH 6.8 and at 40°C. The ultrasonic irradiation (20 kHz, 200 W, 20 min) of I—III was carried out at 0°C under helium gas atmosphere to reduce molecular weights. Each sonicated sample could be fractionated to several fractions, in large quantities, by successive precipitation with acetone. The weight-average molecular weight Mw and degree of polydispersity Mw/Mn were determined for each fraction by the GPC/LALLS method. The molecular weight distribution profile of each sample could be fitted by the logarithmic-normal distribution function.. The intactness of the secondary structure of sonicated triple helices I—III was confirmed by the spectrophotometrically-determined melting temperature of each fraction. From the intrinsic viscosity vs. Mw relation,[η]=KMwα, the exponent α was evaluated for each helix. The chain stiffness of the helical backbone was concluded to decrease in the order of III, II, and I." @default.
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- W2008770982 title "Determination of Molecular Weight Distribution and Spectroscopic and Viscometric Properties of Sonicated Triple-Stranded Polyribonucleotides" @default.
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- W2008770982 doi "https://doi.org/10.1295/polymj.26.303" @default.
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