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• W2008829462 abstract "Human respiratory syncytial virus (HRSV) is an important respiratory pathogens among children between zero-five years old. Host immunity and viral genetic variability are important factors that can make vaccine production difficult. In this work, differences between biological clones of HRSV were detected in clinical samples in the absence and presence of serum collected from children in the convalescent phase of the illness and from their biological mothers. Viral clones were selected by plaque assay in the absence and presence of serum and nucleotide sequences of the G2 and F2 genes of HRSV biological clones were compared. One non-synonymous mutation was found in the F gene (Ile5Asn) in one clone of an HRSV-B sample and one non-synonymous mutation was found in the G gene (Ser291Pro) in four clones of the same HRSV-B sample. Only one of these clones was obtained after treatment with the child's serum. In addition, some synonymous mutations were determined in two clones of the HRSV-A samples. In conclusion, it is possible that minor sequences could be selected by host antibodies contributing to the HRSV evolutionary process, hampering the development of an effective vaccine, since we verify the same codon alteration in absence and presence of human sera in individual clones of BR-85 sample." @default.
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• W2008829462 date "2015-02-01" @default.
• W2008829462 modified "2023-09-27" @default.
• W2008829462 title "Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure" @default.
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• W2008829462 doi "https://doi.org/10.1590/0074-02760140299" @default.
• W2008829462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4371228" @default.
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