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• W2008889055 abstract "Water-soluble (1→3)-β-d-glucans with 1,6-linked branches (SBG) isolated from the cell walls of Saccharomyces cerevisiae were studied by size exclusion chromatography (SEC) with multi-angle laser light scattering (MALLS) detector using dimethylacetamide (DMAc) containing 0.5% (0.12M) LiCl, or dimethyl sulphoxide (DMSO) in the absence and presence of 0.25M LiCl, respectively, as eluents. The aggregating glucan could be dispersed as single chains in both solvents, with chain length distributions in reasonable agreement with results obtained previously with carboxymethylated glucans in aqueous solvent. However, DMAc is preferred over DMSO because of higher sensitivity in MALLS, and also because the latter produces SEC anomalies. SBG dissolves slowly in DMAc/LICl at room temperature, but heating accelerates the process. The rate of depolymerisation of SBG in DMAc/LiCl at high temperatures (70-105°C) was determined as a basis for defining dissolution procedures at elevated temperatures with a minimum of degradation. The result of the investigation is a simple and reliable protocol for preparing unaggregated, fully dissolved and undegraded SBG in DMAc/LiCl, which is well suited as a standard analysis of the molecular weight distribution of SBG-like molecules without chemical derivatization." @default.
• W2008889055 created "2016-06-24" @default.
• W2008889055 creator A5023280182 @default.
• W2008889055 creator A5030416086 @default.
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• W2008889055 date "2013-08-01" @default.
• W2008889055 modified "2023-09-26" @default.
• W2008889055 title "A study of bioactive, branched (1→3)-β-d-glucans in dimethylacetamide/LiCl and dimethyl sulphoxide/LiCl using size-exclusion chromatography with multi-angle light scattering detection" @default.
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• W2008889055 doi "https://doi.org/10.1016/j.chroma.2013.07.002" @default.
• W2008889055 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23891208" @default.
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