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• W2008889483 abstract "Calcium ions are involved in a number of important signal transduction pathways in cells. Cytosolic calcium concentration ([Ca2+]c) can be affected by the activation of Ca2+ channels through the action of ligands such as ATP. The response of [Ca2+]c to ligands may be affected by external factors like magnetic fields. The purpose of this study was to determine if exposure to a static magnetic field (SMF) for 800 s altered the [Ca2+]c response to ATP in undifferentiated HL-60 cells. We sham exposed or field exposed fura-2 loaded HL-60 cells to a SMF of 1, 10, and 100 mT. Cells were activated with ATP 300 s into the exposure. The level of [Ca2+]c was followed before, during, and after field or sham exposure with a ratiometric fluorescence spectroscopy system. It was found that high concentrations of ATP resulted in greater [Ca2+]c responses, but faster recovery to near basal levels. The application of 1, 10, or 100 mT SMF did not affect the [Ca2+]c response to ATP. Future work could examine the effect of a longer SMF exposure on the [Ca2+]c response to ATP. Longer exposures might provide sufficient time for morphological changes in the plasma membrane to occur. Bioelectromagnetics 29:439–446, 2008. © 2008 Wiley-Liss, Inc." @default.
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• W2008889483 date "2008-09-01" @default.
• W2008889483 modified "2023-10-18" @default.
• W2008889483 title "Real-time measurement of cytosolic free calcium concentration in HL-60 cells during static magnetic field exposure and activation by ATP" @default.
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• W2008889483 doi "https://doi.org/10.1002/bem.20409" @default.
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