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- W2008939614 abstract "The aryl hydrocarbon receptor (AHR) gene encodes a ligand-activated transcription factor through which planar halogenated aromatic hydrocarbons (HAHs) such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as well as polynuclear aromatic hydrocarbons (PAHs) cause altered gene expression and toxicity. To understand the role of AHR genetic variability in differential sensitivity to HAHs and PAHs, we are currently studying a population of the teleost Fundulus heteroclitus (Atlantic killifish) that has evolved genetic resistance to the toxic and biochemical effects of these compounds. Here, we report that the killifish AHR1 locus is highly polymorphic and that the frequencies of the major allele types differ between dioxin-sensitive and dioxin-resistant populations. Twenty-five single nucleotide polymorphisms (SNPs), nine of which are non-synonymous, were identified in the AHR1 coding sequence. Seven identified alleles were assigned to three groups, designated AHR1*1, AHR1*2 and AHR1*3. AHR1*1 alleles were under-represented in a population of dioxin- and polychlorinated biphenyl (PCB)-resistant fish from a PCB-contaminated Superfund site (New Bedford Harbor, Massachusetts, USA) compared to dioxin-sensitive fish from a less contaminated reference site (Scorton Creek, Massachusetts, USA). To determine the possible role of these AHR1 variants in differential HAH sensitivity, we expressed representative variant proteins from the two most divergent allelic groups (AHR1*1 and AHR1*3) by in-vitro transcription and translation and assessed their functional properties. AHR1*1A and AHR1*3A proteins displayed similar binding capacities and affinities for [3H]TCDD. In transient transfection assays using mammalian cells, AHR1*1A and AHR1*3A exhibited similar abilities to support TCDD-dependent transactivation of a luciferase reporter gene under control of AHR-responsive enhancer elements. We discuss the possibility of other functional differences in AHR1 variants or their interaction with other killifish loci (AHR2, AHRR) that may contribute to differences in dioxin sensitivity." @default.
- W2008939614 created "2016-06-24" @default.
- W2008939614 creator A5007449265 @default.
- W2008939614 creator A5018985066 @default.
- W2008939614 creator A5019234030 @default.
- W2008939614 creator A5080521752 @default.
- W2008939614 date "2004-02-01" @default.
- W2008939614 modified "2023-10-15" @default.
- W2008939614 title "Aryl hydrocarbon receptor polymorphisms and dioxin resistance in Atlantic killifish (Fundulus heteroclitus)" @default.
- W2008939614 cites W1481507011 @default.
- W2008939614 cites W1537989154 @default.
- W2008939614 cites W1576236282 @default.
- W2008939614 cites W1585045286 @default.
- W2008939614 cites W1955324140 @default.
- W2008939614 cites W1964770503 @default.
- W2008939614 cites W1964969050 @default.
- W2008939614 cites W1965468893 @default.
- W2008939614 cites W1968906511 @default.
- W2008939614 cites W1969906168 @default.
- W2008939614 cites W1976065442 @default.
- W2008939614 cites W1976229657 @default.
- W2008939614 cites W1980214543 @default.
- W2008939614 cites W1980552888 @default.
- W2008939614 cites W1981407146 @default.
- W2008939614 cites W1982091422 @default.
- W2008939614 cites W1982599952 @default.
- W2008939614 cites W1983585455 @default.
- W2008939614 cites W1987066837 @default.
- W2008939614 cites W1987666378 @default.
- W2008939614 cites W1989293350 @default.
- W2008939614 cites W1993902331 @default.
- W2008939614 cites W1994623914 @default.
- W2008939614 cites W1994857418 @default.
- W2008939614 cites W2009225735 @default.
- W2008939614 cites W2015200660 @default.
- W2008939614 cites W2015645721 @default.
- W2008939614 cites W2020620545 @default.
- W2008939614 cites W2020991956 @default.
- W2008939614 cites W2027656505 @default.
- W2008939614 cites W2031344638 @default.
- W2008939614 cites W2032542284 @default.
- W2008939614 cites W2040475011 @default.
- W2008939614 cites W2043625689 @default.
- W2008939614 cites W2045931107 @default.
- W2008939614 cites W2046271197 @default.
- W2008939614 cites W2046665066 @default.
- W2008939614 cites W2048160348 @default.
- W2008939614 cites W2058763547 @default.
- W2008939614 cites W2059565720 @default.
- W2008939614 cites W2067937670 @default.
- W2008939614 cites W2068158033 @default.
- W2008939614 cites W2068489442 @default.
- W2008939614 cites W2070193529 @default.
- W2008939614 cites W2070730833 @default.
- W2008939614 cites W2071460875 @default.
- W2008939614 cites W2071971189 @default.
- W2008939614 cites W2076282760 @default.
- W2008939614 cites W2082809617 @default.
- W2008939614 cites W2083910374 @default.
- W2008939614 cites W2087332917 @default.
- W2008939614 cites W2088775939 @default.
- W2008939614 cites W2089362925 @default.
- W2008939614 cites W2094234986 @default.
- W2008939614 cites W2094762907 @default.
- W2008939614 cites W2097382368 @default.
- W2008939614 cites W2100180451 @default.
- W2008939614 cites W2100326496 @default.
- W2008939614 cites W2108131387 @default.
- W2008939614 cites W2109610746 @default.
- W2008939614 cites W2111023683 @default.
- W2008939614 cites W2112656147 @default.
- W2008939614 cites W2114629114 @default.
- W2008939614 cites W2114757072 @default.
- W2008939614 cites W2118517192 @default.
- W2008939614 cites W2120473115 @default.
- W2008939614 cites W2124739658 @default.
- W2008939614 cites W2128673805 @default.
- W2008939614 cites W2128692282 @default.
- W2008939614 cites W2129873761 @default.
- W2008939614 cites W2133972226 @default.
- W2008939614 cites W2136306660 @default.
- W2008939614 cites W2137118500 @default.
- W2008939614 cites W2137135419 @default.
- W2008939614 cites W2138913971 @default.
- W2008939614 cites W2139322272 @default.
- W2008939614 cites W2144368480 @default.
- W2008939614 cites W2144376159 @default.
- W2008939614 cites W2146124483 @default.
- W2008939614 cites W2148440360 @default.
- W2008939614 cites W2152752486 @default.
- W2008939614 cites W2154457653 @default.
- W2008939614 cites W2160670052 @default.
- W2008939614 cites W2165627850 @default.
- W2008939614 cites W2171399687 @default.
- W2008939614 cites W2179885821 @default.
- W2008939614 cites W2326890067 @default.
- W2008939614 cites W2329295337 @default.
- W2008939614 cites W2336672860 @default.