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- W2009013480 abstract "<b>Background:</b> There is increasing interest in DNA methylation and in its implication in transcriptional gene silencing, a phenomenon commonly seen in human cancer. <b>Aims:</b> To develop a new method that would allow quantitative DNA methylation analysis in a large range of clinical samples, independently of the processing protocol. <b>Methods:</b> A methylation sensitive dot blot assay (MS-DBA) was developed, which is quantitative and combines bisulfite modification, PCR amplification using primers without CpG sites, and dot blot analysis with two probes specific for methylated and unmethylated DNA. <b>Results:</b> The established method was used to study methylation of the hTERT, APC, and p16 promoter regions in microdissected, formalin fixed and paraffin wax embedded tissues. <b>Conclusions:</b> MS-DBA is a sensitive, specific, and quantitative approach to analyse DNA methylation in a variety of frozen or fixed tissues. Moreover, MS-DBA is rapid, easy to perform, and permits the screening of a large panel of samples in one experiment. Thus, MS-DBA can facilitate the routine analysis of DNA methylation in all types of clinical samples." @default.
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- W2009013480 date "2005-02-01" @default.
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- W2009013480 title "A methylation sensitive dot blot assay (MS-DBA) for the quantitative analysis of DNA methylation in clinical samples" @default.
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- W2009013480 doi "https://doi.org/10.1136/jcp.2004.021147" @default.
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