Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009092008> ?p ?o ?g. }
- W2009092008 abstract "Abstract Background Biodiesel or ethanol derived from lipids or starch produced by microalgae may overcome many of the sustainability challenges previously ascribed to petroleum-based fuels and first generation plant-based biofuels. The paucity of microalgae genome sequences, however, limits gene-based biofuel feedstock optimization studies. Here we describe the sequencing and de novo transcriptome assembly for the non-model microalgae species, Dunaliella tertiolecta , and identify pathways and genes of importance related to biofuel production. Results Next generation DNA pyrosequencing technology applied to D. tertiolecta transcripts produced 1,363,336 high quality reads with an average length of 400 bases. Following quality and size trimming, ~ 45% of the high quality reads were assembled into 33,307 isotigs with a 31-fold coverage and 376,482 singletons. Assembled sequences and singletons were subjected to BLAST similarity searches and annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology (KO) identifiers. These analyses identified the majority of lipid and starch biosynthesis and catabolism pathways in D. tertiolecta . Conclusions The construction of metabolic pathways involved in the biosynthesis and catabolism of fatty acids, triacylglycrols, and starch in D. tertiolecta as well as the assembled transcriptome provide a foundation for the molecular genetics and functional genomics required to direct metabolic engineering efforts that seek to enhance the quantity and character of microalgae-based biofuel feedstock." @default.
- W2009092008 created "2016-06-24" @default.
- W2009092008 creator A5035681909 @default.
- W2009092008 creator A5057853675 @default.
- W2009092008 creator A5062959054 @default.
- W2009092008 creator A5075958882 @default.
- W2009092008 date "2011-03-14" @default.
- W2009092008 modified "2023-10-16" @default.
- W2009092008 title "Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels" @default.
- W2009092008 cites W1965569434 @default.
- W2009092008 cites W1974116356 @default.
- W2009092008 cites W1979832917 @default.
- W2009092008 cites W1981194760 @default.
- W2009092008 cites W1985484001 @default.
- W2009092008 cites W1988177221 @default.
- W2009092008 cites W1991283899 @default.
- W2009092008 cites W1996577510 @default.
- W2009092008 cites W2002573790 @default.
- W2009092008 cites W2005392171 @default.
- W2009092008 cites W2006978978 @default.
- W2009092008 cites W2010364121 @default.
- W2009092008 cites W2015289708 @default.
- W2009092008 cites W2018637188 @default.
- W2009092008 cites W2019953979 @default.
- W2009092008 cites W2021046287 @default.
- W2009092008 cites W2021429484 @default.
- W2009092008 cites W2026995009 @default.
- W2009092008 cites W2028656888 @default.
- W2009092008 cites W2033283923 @default.
- W2009092008 cites W2036814808 @default.
- W2009092008 cites W2038195922 @default.
- W2009092008 cites W2038445570 @default.
- W2009092008 cites W2044115077 @default.
- W2009092008 cites W2047281856 @default.
- W2009092008 cites W2050980391 @default.
- W2009092008 cites W2055043387 @default.
- W2009092008 cites W2057735448 @default.
- W2009092008 cites W2060415232 @default.
- W2009092008 cites W2066443005 @default.
- W2009092008 cites W2067522602 @default.
- W2009092008 cites W2069775531 @default.
- W2009092008 cites W2074030909 @default.
- W2009092008 cites W2074511373 @default.
- W2009092008 cites W2088139254 @default.
- W2009092008 cites W2091922579 @default.
- W2009092008 cites W2103017472 @default.
- W2009092008 cites W2105106534 @default.
- W2009092008 cites W2108672120 @default.
- W2009092008 cites W2114874948 @default.
- W2009092008 cites W2116224228 @default.
- W2009092008 cites W2120780572 @default.
- W2009092008 cites W2122437201 @default.
- W2009092008 cites W2123405262 @default.
- W2009092008 cites W2128799561 @default.
- W2009092008 cites W2132611682 @default.
- W2009092008 cites W2133579817 @default.
- W2009092008 cites W2135575973 @default.
- W2009092008 cites W2143439482 @default.
- W2009092008 cites W2144640901 @default.
- W2009092008 cites W2145811052 @default.
- W2009092008 cites W2146384526 @default.
- W2009092008 cites W2153922477 @default.
- W2009092008 cites W2153983368 @default.
- W2009092008 cites W2156778513 @default.
- W2009092008 cites W2159482845 @default.
- W2009092008 cites W2159785760 @default.
- W2009092008 cites W2163917364 @default.
- W2009092008 cites W2165061305 @default.
- W2009092008 cites W2171311206 @default.
- W2009092008 cites W2494884038 @default.
- W2009092008 cites W4256409604 @default.
- W2009092008 cites W4256435802 @default.
- W2009092008 doi "https://doi.org/10.1186/1471-2164-12-148" @default.
- W2009092008 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3061936" @default.
- W2009092008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21401935" @default.
- W2009092008 hasPublicationYear "2011" @default.
- W2009092008 type Work @default.
- W2009092008 sameAs 2009092008 @default.
- W2009092008 citedByCount "245" @default.
- W2009092008 countsByYear W20090920082012 @default.
- W2009092008 countsByYear W20090920082013 @default.
- W2009092008 countsByYear W20090920082014 @default.
- W2009092008 countsByYear W20090920082015 @default.
- W2009092008 countsByYear W20090920082016 @default.
- W2009092008 countsByYear W20090920082017 @default.
- W2009092008 countsByYear W20090920082018 @default.
- W2009092008 countsByYear W20090920082019 @default.
- W2009092008 countsByYear W20090920082020 @default.
- W2009092008 countsByYear W20090920082021 @default.
- W2009092008 countsByYear W20090920082022 @default.
- W2009092008 countsByYear W20090920082023 @default.
- W2009092008 crossrefType "journal-article" @default.
- W2009092008 hasAuthorship W2009092008A5035681909 @default.
- W2009092008 hasAuthorship W2009092008A5057853675 @default.
- W2009092008 hasAuthorship W2009092008A5062959054 @default.
- W2009092008 hasAuthorship W2009092008A5075958882 @default.
- W2009092008 hasBestOaLocation W20090920081 @default.
- W2009092008 hasConcept C104317684 @default.
- W2009092008 hasConcept C141231307 @default.
- W2009092008 hasConcept C150194340 @default.