Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009096988> ?p ?o ?g. }
- W2009096988 endingPage "71" @default.
- W2009096988 startingPage "64" @default.
- W2009096988 abstract "Background Cyclic AMP (cAMP) plays a pivotal role in insulin secretion induced by incretins. The effects of the second messenger extend to many sites and there has been much controversy on the mechanisms. The aim of this study was to examine how cAMP amplified insulin exocytosis. Methods Rat islets were permeabilized with α-toxin to measure insulin exocytosis in the fixed conditions of Ca2+ and ATP. The effects of several agents on insulin exocytosis were observed in perifusion experiments. Results Cyclic AMP enhanced the Ca2+-induced insulin release by around 30%, independent of Ca2+ concentrations between 10 and 3000 nmol/L. A cAMP-GEF selective cAMP analogue, 8-(4-chloro-phenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate, also amplified insulin release. The effect disappeared in the absence of ATP. Conversely, cAMP-independent gradual increase in insulin release was observed with ATP. These results suggested that the site of action of cAMP-GEF existed proximal to that of ATP. An analogue selective to PKA, N6-Benzoyladenosine-3′,5′-cyclic monophosphate, had little effect. Also, a PKA-selective inhibitor, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide, reduced insulin releases induced by 1000 nmol/L Ca2+, but did not influence the relative increase produced by Ca2+ and cAMP. Conclusion Cyclic AMP potentiated Ca2+ and ATP-induced exocytosis to a similar relative extent independent of Ca2+ concentrations. The process appeared to be mainly mediated by cAMP-GEF. In addition, the cAMP/cAMP-GEF pathway may enhance insulin release by replenishing the readily releasable pool. Copyright © 2005 John Wiley & Sons, Ltd." @default.
- W2009096988 created "2016-06-24" @default.
- W2009096988 creator A5005785362 @default.
- W2009096988 creator A5010323539 @default.
- W2009096988 creator A5019018249 @default.
- W2009096988 creator A5052361715 @default.
- W2009096988 creator A5075224778 @default.
- W2009096988 creator A5083986881 @default.
- W2009096988 date "2005-01-01" @default.
- W2009096988 modified "2023-10-11" @default.
- W2009096988 title "Cyclic AMP/cAMP-GEF pathway amplifies insulin exocytosis induced by Ca2+ and ATP in rat islet β-cells" @default.
- W2009096988 cites W1513753334 @default.
- W2009096988 cites W1514212027 @default.
- W2009096988 cites W1531564543 @default.
- W2009096988 cites W1571595997 @default.
- W2009096988 cites W1799216602 @default.
- W2009096988 cites W1866455211 @default.
- W2009096988 cites W1885709862 @default.
- W2009096988 cites W1963820874 @default.
- W2009096988 cites W1968031607 @default.
- W2009096988 cites W1968498807 @default.
- W2009096988 cites W1988196562 @default.
- W2009096988 cites W1996164493 @default.
- W2009096988 cites W1999041565 @default.
- W2009096988 cites W2001180476 @default.
- W2009096988 cites W2006402504 @default.
- W2009096988 cites W2009592438 @default.
- W2009096988 cites W2013910844 @default.
- W2009096988 cites W2014716037 @default.
- W2009096988 cites W2016266703 @default.
- W2009096988 cites W2016898556 @default.
- W2009096988 cites W2017518223 @default.
- W2009096988 cites W2017631187 @default.
- W2009096988 cites W2021467665 @default.
- W2009096988 cites W2024042905 @default.
- W2009096988 cites W2028946878 @default.
- W2009096988 cites W2032168988 @default.
- W2009096988 cites W2040278047 @default.
- W2009096988 cites W2044162798 @default.
- W2009096988 cites W2048032465 @default.
- W2009096988 cites W2050046962 @default.
- W2009096988 cites W2058591402 @default.
- W2009096988 cites W2067962261 @default.
- W2009096988 cites W2076924219 @default.
- W2009096988 cites W2078037416 @default.
- W2009096988 cites W2078312203 @default.
- W2009096988 cites W2088500989 @default.
- W2009096988 cites W2101695647 @default.
- W2009096988 cites W2121827210 @default.
- W2009096988 cites W2126578889 @default.
- W2009096988 cites W2143563119 @default.
- W2009096988 cites W2145824107 @default.
- W2009096988 cites W2160184086 @default.
- W2009096988 cites W2163585457 @default.
- W2009096988 cites W2164561590 @default.
- W2009096988 cites W2166287438 @default.
- W2009096988 cites W2167324760 @default.
- W2009096988 cites W2171483278 @default.
- W2009096988 cites W2172282347 @default.
- W2009096988 cites W2341732097 @default.
- W2009096988 cites W2398641394 @default.
- W2009096988 cites W4247904703 @default.
- W2009096988 doi "https://doi.org/10.1002/dmrr.580" @default.
- W2009096988 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16028217" @default.
- W2009096988 hasPublicationYear "2005" @default.
- W2009096988 type Work @default.
- W2009096988 sameAs 2009096988 @default.
- W2009096988 citedByCount "26" @default.
- W2009096988 countsByYear W20090969882012 @default.
- W2009096988 countsByYear W20090969882013 @default.
- W2009096988 countsByYear W20090969882014 @default.
- W2009096988 countsByYear W20090969882016 @default.
- W2009096988 countsByYear W20090969882017 @default.
- W2009096988 countsByYear W20090969882018 @default.
- W2009096988 countsByYear W20090969882022 @default.
- W2009096988 crossrefType "journal-article" @default.
- W2009096988 hasAuthorship W2009096988A5005785362 @default.
- W2009096988 hasAuthorship W2009096988A5010323539 @default.
- W2009096988 hasAuthorship W2009096988A5019018249 @default.
- W2009096988 hasAuthorship W2009096988A5052361715 @default.
- W2009096988 hasAuthorship W2009096988A5075224778 @default.
- W2009096988 hasAuthorship W2009096988A5083986881 @default.
- W2009096988 hasBestOaLocation W20090969881 @default.
- W2009096988 hasConcept C126322002 @default.
- W2009096988 hasConcept C134018914 @default.
- W2009096988 hasConcept C163235415 @default.
- W2009096988 hasConcept C165220095 @default.
- W2009096988 hasConcept C170493617 @default.
- W2009096988 hasConcept C175969161 @default.
- W2009096988 hasConcept C180526460 @default.
- W2009096988 hasConcept C185592680 @default.
- W2009096988 hasConcept C24998067 @default.
- W2009096988 hasConcept C2779276759 @default.
- W2009096988 hasConcept C2779306644 @default.
- W2009096988 hasConcept C2780069284 @default.
- W2009096988 hasConcept C2781298581 @default.
- W2009096988 hasConcept C49039625 @default.
- W2009096988 hasConcept C71924100 @default.