Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009105277> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2009105277 endingPage "331" @default.
- W2009105277 startingPage "316" @default.
- W2009105277 abstract "Heat-induced cell death and apoptosis were studied with respect to intracellular ATP. Studies on the relationship between hyperthermic cell-killing at 44 degrees C and cellular ATP levels in four cell lines grown as monolayers and six cell lines grown in suspension showed good correlations between cellular ATP levels and the sensitivity to heat. D(0) values (the dose required to reduce survival in the linear portion of the response by 63%) linearly increased with an increase in cellular ATP levels. No such changes in sensitivity to heat were observed between the cells cultured at different cell densities, regardless of the change in the cellular ATP level. These results suggest that cellular intrinsic ability to supply ATP rather than the level of pooled ATP per se is responsible for the thermal response. Heat-induced apoptosis in L5178Y cells was observed following treatment at 42 degrees C for 70 min, 44 degrees C for 20 min or 47 degrees C for 3 min, which corresponded to surviving fractions of 25, 0.6 and 0.8%, respectively, but not at 47 degrees C for 20 min, indicating that mild heat shock induced apoptosis. 2-deoxyglucose (2DG) and 2,4-dinitrophenol (DNP) increased the sensitivity to heat and affected the mode of cell death. Cells treated with 2DG and DNP (2DG/DNP) were heated at 42 degrees C for 20 min, and then incubated at 37 degrees C for up to 2h in the presence or absence of 2DG/DNP. In the absence of 2DG/DNP, the cellular ATP level recovered to 76% of the control level and DNA ladder formation was observed, whereas in the presence of 2DG/DNP, the cellular ATP level was further decreased (3-7% of the control) and no DNA fragmentation was detected. These results suggest that the inhibition of ATP synthesis is closely associated with the enhancement of sensitivity to heat and that ATP is required for the induction of apoptosis." @default.
- W2009105277 created "2016-06-24" @default.
- W2009105277 creator A5010316369 @default.
- W2009105277 creator A5079589557 @default.
- W2009105277 creator A5084840015 @default.
- W2009105277 creator A5086858337 @default.
- W2009105277 date "2002-07-01" @default.
- W2009105277 modified "2023-09-27" @default.
- W2009105277 title "Role of ATP in the sensitivity to heat and the induction of apoptosis in mammalian cells" @default.
- W2009105277 doi "https://doi.org/10.1080/02656730210123552" @default.
- W2009105277 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12079587" @default.
- W2009105277 hasPublicationYear "2002" @default.
- W2009105277 type Work @default.
- W2009105277 sameAs 2009105277 @default.
- W2009105277 citedByCount "6" @default.
- W2009105277 countsByYear W20091052772012 @default.
- W2009105277 countsByYear W20091052772019 @default.
- W2009105277 countsByYear W20091052772023 @default.
- W2009105277 crossrefType "journal-article" @default.
- W2009105277 hasAuthorship W2009105277A5010316369 @default.
- W2009105277 hasAuthorship W2009105277A5079589557 @default.
- W2009105277 hasAuthorship W2009105277A5084840015 @default.
- W2009105277 hasAuthorship W2009105277A5086858337 @default.
- W2009105277 hasConcept C104317684 @default.
- W2009105277 hasConcept C12554922 @default.
- W2009105277 hasConcept C137984847 @default.
- W2009105277 hasConcept C1491633281 @default.
- W2009105277 hasConcept C153911025 @default.
- W2009105277 hasConcept C185592680 @default.
- W2009105277 hasConcept C190283241 @default.
- W2009105277 hasConcept C205260736 @default.
- W2009105277 hasConcept C31573885 @default.
- W2009105277 hasConcept C54355233 @default.
- W2009105277 hasConcept C55493867 @default.
- W2009105277 hasConcept C62112901 @default.
- W2009105277 hasConcept C79879829 @default.
- W2009105277 hasConcept C81885089 @default.
- W2009105277 hasConcept C86803240 @default.
- W2009105277 hasConcept C95444343 @default.
- W2009105277 hasConceptScore W2009105277C104317684 @default.
- W2009105277 hasConceptScore W2009105277C12554922 @default.
- W2009105277 hasConceptScore W2009105277C137984847 @default.
- W2009105277 hasConceptScore W2009105277C1491633281 @default.
- W2009105277 hasConceptScore W2009105277C153911025 @default.
- W2009105277 hasConceptScore W2009105277C185592680 @default.
- W2009105277 hasConceptScore W2009105277C190283241 @default.
- W2009105277 hasConceptScore W2009105277C205260736 @default.
- W2009105277 hasConceptScore W2009105277C31573885 @default.
- W2009105277 hasConceptScore W2009105277C54355233 @default.
- W2009105277 hasConceptScore W2009105277C55493867 @default.
- W2009105277 hasConceptScore W2009105277C62112901 @default.
- W2009105277 hasConceptScore W2009105277C79879829 @default.
- W2009105277 hasConceptScore W2009105277C81885089 @default.
- W2009105277 hasConceptScore W2009105277C86803240 @default.
- W2009105277 hasConceptScore W2009105277C95444343 @default.
- W2009105277 hasIssue "4" @default.
- W2009105277 hasLocation W20091052771 @default.
- W2009105277 hasLocation W20091052772 @default.
- W2009105277 hasOpenAccess W2009105277 @default.
- W2009105277 hasPrimaryLocation W20091052771 @default.
- W2009105277 hasRelatedWork W1234816868 @default.
- W2009105277 hasRelatedWork W1981686547 @default.
- W2009105277 hasRelatedWork W2030883789 @default.
- W2009105277 hasRelatedWork W2069300823 @default.
- W2009105277 hasRelatedWork W2070291783 @default.
- W2009105277 hasRelatedWork W2071063451 @default.
- W2009105277 hasRelatedWork W2147761319 @default.
- W2009105277 hasRelatedWork W2353415333 @default.
- W2009105277 hasRelatedWork W2355259601 @default.
- W2009105277 hasRelatedWork W2913645037 @default.
- W2009105277 hasVolume "18" @default.
- W2009105277 isParatext "false" @default.
- W2009105277 isRetracted "false" @default.
- W2009105277 magId "2009105277" @default.
- W2009105277 workType "article" @default.