FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009134055> ?p ?o ?g. }

• W2009134055 endingPage "e31857" @default.
• W2009134055 startingPage "e31857" @default.
• W2009134055 abstract "Background Erythropoietin receptors have been identified in human skeletal muscle tissue, but downstream signal transduction has not been investigated. We therefore studied in vivo effects of systemic erythropoietin exposure in human skeletal muscle. Methodology/Principal Findings The protocols involved 1) acute effects of a single bolus injection of erythropoietin followed by consecutive muscle biopsies for 1–10 hours, and 2) a separate study with prolonged administration for 16 days with biopsies obtained before and after. The presence of erythropoietin receptors in muscle tissue as well as activation of Epo signalling pathways (STAT5, MAPK, Akt, IKK) were analysed by western blotting. Changes in muscle protein profiles after prolonged erythropoietin treatment were evaluated by 2D gel-electrophoresis and mass spectrometry. The presence of the erythropoietin receptor in skeletal muscle was confirmed, by the M20 but not the C20 antibody. However, no significant changes in phosphorylation of the Epo-R, STAT5, MAPK, Akt, Lyn, IKK, and p70S6K after erythropoietin administration were detected. The level of 8 protein spots were significantly altered after 16 days of rHuEpo treatment; one isoform of myosin light chain 3 and one of desmin/actin were decreased, while three isoforms of creatine kinase and two of glyceraldehyd-3-phosphate dehydrogenase were increased. Conclusions/Significance Acute exposure to recombinant human erythropoietin is not associated by detectable activation of the Epo-R or downstream signalling targets in human skeletal muscle in the resting situation, whereas more prolonged exposure induces significant changes in the skeletal muscle proteome. The absence of functional Epo receptor activity in human skeletal muscle indicates that the long-term effects are indirect and probably related to an increased oxidative capacity in this tissue." @default.
• W2009134055 created "2016-06-24" @default.
• W2009134055 creator A5021672623 @default.
• W2009134055 creator A5039041871 @default.
• W2009134055 creator A5054006122 @default.
• W2009134055 creator A5061293714 @default.
• W2009134055 creator A5064270055 @default.
• W2009134055 creator A5064798252 @default.
• W2009134055 creator A5066850019 @default.
• W2009134055 creator A5083032630 @default.
• W2009134055 creator A5088598369 @default.
• W2009134055 creator A5090850055 @default.
• W2009134055 date "2012-02-22" @default.
• W2009134055 modified "2023-09-27" @default.
• W2009134055 title "Evaluation of Functional Erythropoietin Receptor Status in Skeletal Muscle In Vivo: Acute and Prolonged Studies in Healthy Human Subjects" @default.
• W2009134055 cites W1484942319 @default.
• W2009134055 cites W1497672261 @default.
• W2009134055 cites W1886472157 @default.
• W2009134055 cites W1899050407 @default.
• W2009134055 cites W1963750108 @default.
• W2009134055 cites W1965419075 @default.
• W2009134055 cites W1971630204 @default.
• W2009134055 cites W1974694639 @default.
• W2009134055 cites W1979330813 @default.
• W2009134055 cites W1980203065 @default.
• W2009134055 cites W1993639183 @default.
• W2009134055 cites W1999956648 @default.
• W2009134055 cites W2005334673 @default.
• W2009134055 cites W2029313715 @default.
• W2009134055 cites W2040242673 @default.
• W2009134055 cites W2043981867 @default.
• W2009134055 cites W2058930880 @default.
• W2009134055 cites W2059175990 @default.
• W2009134055 cites W2075047779 @default.
• W2009134055 cites W2076805509 @default.
• W2009134055 cites W2077316495 @default.
• W2009134055 cites W2079527824 @default.
• W2009134055 cites W2081653815 @default.
• W2009134055 cites W2082490162 @default.
• W2009134055 cites W2083525711 @default.
• W2009134055 cites W2084661996 @default.
• W2009134055 cites W2125394545 @default.
• W2009134055 cites W2128157205 @default.
• W2009134055 cites W2129637610 @default.
• W2009134055 cites W2134251116 @default.
• W2009134055 cites W2140654407 @default.
• W2009134055 cites W2156043827 @default.
• W2009134055 cites W2160521073 @default.
• W2009134055 cites W2167788515 @default.
• W2009134055 cites W2171768996 @default.
• W2009134055 cites W2408339784 @default.
• W2009134055 doi "https://doi.org/10.1371/journal.pone.0031857" @default.
• W2009134055 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3285196" @default.
• W2009134055 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22384088" @default.
• W2009134055 hasPublicationYear "2012" @default.
• W2009134055 type Work @default.
• W2009134055 sameAs 2009134055 @default.
• W2009134055 citedByCount "15" @default.
• W2009134055 countsByYear W20091340552012 @default.
• W2009134055 countsByYear W20091340552013 @default.
• W2009134055 countsByYear W20091340552014 @default.
• W2009134055 countsByYear W20091340552015 @default.
• W2009134055 countsByYear W20091340552016 @default.
• W2009134055 countsByYear W20091340552017 @default.
• W2009134055 crossrefType "journal-article" @default.
• W2009134055 hasAuthorship W2009134055A5021672623 @default.
• W2009134055 hasAuthorship W2009134055A5039041871 @default.
• W2009134055 hasAuthorship W2009134055A5054006122 @default.
• W2009134055 hasAuthorship W2009134055A5061293714 @default.
• W2009134055 hasAuthorship W2009134055A5064270055 @default.
• W2009134055 hasAuthorship W2009134055A5064798252 @default.
• W2009134055 hasAuthorship W2009134055A5066850019 @default.
• W2009134055 hasAuthorship W2009134055A5083032630 @default.
• W2009134055 hasAuthorship W2009134055A5088598369 @default.
• W2009134055 hasAuthorship W2009134055A5090850055 @default.
• W2009134055 hasBestOaLocation W20091340551 @default.
• W2009134055 hasConcept C126322002 @default.
• W2009134055 hasConcept C134018914 @default.
• W2009134055 hasConcept C147447768 @default.
• W2009134055 hasConcept C170493617 @default.
• W2009134055 hasConcept C204232928 @default.
• W2009134055 hasConcept C2778534260 @default.
• W2009134055 hasConcept C2779072444 @default.
• W2009134055 hasConcept C2779959927 @default.
• W2009134055 hasConcept C31102739 @default.
• W2009134055 hasConcept C6997183 @default.
• W2009134055 hasConcept C71924100 @default.
• W2009134055 hasConcept C86803240 @default.
• W2009134055 hasConcept C95444343 @default.
• W2009134055 hasConceptScore W2009134055C126322002 @default.
• W2009134055 hasConceptScore W2009134055C134018914 @default.
• W2009134055 hasConceptScore W2009134055C147447768 @default.
• W2009134055 hasConceptScore W2009134055C170493617 @default.
• W2009134055 hasConceptScore W2009134055C204232928 @default.
• W2009134055 hasConceptScore W2009134055C2778534260 @default.
• W2009134055 hasConceptScore W2009134055C2779072444 @default.
• W2009134055 hasConceptScore W2009134055C2779959927 @default.
• W2009134055 hasConceptScore W2009134055C31102739 @default.

• next