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- W2009142018 abstract "ABSTRACT Francisella tularensis is the causative agent of tularemia, a severe zoonotic infectious disease. The natural reservoir of the bacteria is not yet known precisely, but it may persist for over a year in water or mud. It is naturally maintained and spread by various terrestrial and aquatic mammals, and outbreaks in humans are often paralleled by enzootics or epizootics. The reasons for this seem to be multifactorial, though not well understood. Therefore, for epidemiological and outbreak investigations, a rapid hand‐held assay would be helpful. Here we described a column‐based immunofiltration assay called ABICAP, which has several characteristics that permit its application under field conditions. The assay can be performed within 25 min, with a detection limit of 10 3 bacteria. Further validation of the assay included the analytical range, precision, detection limit and performance for different human, animal and environmental sample matrices, accuracy, recovery, reproducibility and stability. PRACTICAL APPLICATIONS The assay is an interesting tool applicable for the rapid detection of F. tularensis in various specimens under laboratory and field conditions. It can be used for the identification of reservoirs of F. tularensis in epidemiological studies, for the detection of sources of infection in outbreak situations of tularemia and in laboratory investigation of animal and human specimens. The methodological platform can be adapted to the detection of other microbiological agents and antigens." @default.
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- W2009142018 date "2008-02-27" @default.
- W2009142018 modified "2023-10-16" @default.
- W2009142018 title "RAPID DETECTION OF FRANCISELLA TULARENSIS BY THE IMMUNOAFFINITY ASSAY ABICAP IN ENVIRONMENTAL AND HUMAN SAMPLES" @default.
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- W2009142018 doi "https://doi.org/10.1111/j.1745-4581.2008.00114.x" @default.
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