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• W2009147973 abstract "In 1900, Michaelis used Janus green B as a supravital stain (20). He observed that filamentous structures within the cytoplasm of the cell stained with this dye and he recognized that these structures were similar to the elementary filaments described by Altmann in 1890 (1). Michaeis had specified that the diethyl derivative of Janus green was necessary for supravital staining. However, a number of subsequent investigators were unsuccessful in using this dye as a supravital stain, presumably because they did not use the diethyl derivative (10). Bensley reintroduced the use of Janus green B as a supravital stain (4). He was careful to use the diethyl derivative and he showed that the structures which stained with Janus green B were identical with the structures which Benda called mitochondria (3). The Janus green B staining reaction is oxygen dependent (5, 6), for although selective staining of mitochondria appears under partial anaerobic conditions, these structures become decolorized when all of the oxygen is removed. The Janus green B staining reaction is reversibly inhibited by cyanide (18, 19); when cyanide is added to supravitally stained mitochondria they become colorless whereas when the cyanide is subsequently removed by washing, the mitochondna regain their blue color. In vitro studies with Janus green B have indicated that this dye is reduced by the lactic dehydrogenase enzyme system (2). All of these observations suggest that Janus green B staining is dependent upon the enzyme activities of the cell. In order to clarify the enzymatic mechanism involved in the Janus green B staining reaction, we have undertaken a systematic study of the enzyme systems which are capable of reacting with Janus green B and its reduction products. Several preliminary notes have been published (7, 14, 15) and three completed papers are in press (16, 8, 9). Further studies on the reduction of Janus green B by the isolated cell components and on the binding of Janus green B to proteins and to isolated cell components are in progress and will be published soon (17)." @default.
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• W2009147973 date "1953-07-01" @default.
• W2009147973 modified "2023-09-27" @default.
• W2009147973 title "STUDIES ON THE ENZYMATIC BASIS FOR THE JANUS GREEN B STAINING REACTION" @default.
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• W2009147973 doi "https://doi.org/10.1177/1.4.234" @default.
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