FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009152893> ?p ?o ?g. }

• W2009152893 endingPage "39" @default.
• W2009152893 startingPage "33" @default.
• W2009152893 abstract "An acidic protease was extracted from acetone powder of the viscera of bolti fish (Tilapia nilotica) in acidified distilled water and precipitated from the resulting extract by ammonium sulfate followed by dialysis and its kinetics studied. The crude enzyme was purified using gel filtration; its homogeneity and molecular weight were studied. The enzyme showed highest activity and purification-fold when precipitated at 40–60% ammonium sulfate. Purification fold and activity were increased after purification by dialysis and gel filtration on Sephadex G-100. Homogeneity studies, by polyacrylamide gel electrophoresis, illustrated that the enzyme was homogeneous only after purification by the gel filtration step. Sodium dodecyl sulphate polyacrylamide gel electrophoresis showed a molecular weight of 31.0 kDa. The optimal pH and optimal temperature were 2.5 and 35°C, respectively. The enzyme showed pH stability between 2 and 6. It retained more than 50% of its activity after heating between 50 and 60°C for 30 min, and 40.2 and 74.9% after heating between the same temperatures for 120 min. The Km and Vmax values of enzyme were 0.77 mM and 2.22 mM/min, respectively, while the catalysis efficiency (Vmax/Km) was 2.88. A high inhibition percentage of total enzyme activity was obtained when the enzyme was incubated with 50 mM of both soybean trypsin inhibitor and ethylenediaminetetraacetic acid. The presence of either NaCl or CaCl2 at 10 mM concentration increased the enzyme activity by 20.5% and 31.2%, respectively." @default.
• W2009152893 created "2016-06-24" @default.
• W2009152893 creator A5033197146 @default.
• W2009152893 creator A5037716507 @default.
• W2009152893 creator A5051833216 @default.
• W2009152893 creator A5089970167 @default.
• W2009152893 date "2004-06-01" @default.
• W2009152893 modified "2023-09-25" @default.
• W2009152893 title "Purification and characterization of an acidic protease from the viscera of bolti fish (Tilapia nilotica)" @default.
• W2009152893 cites W1591894515 @default.
• W2009152893 cites W1775749144 @default.
• W2009152893 cites W2016182813 @default.
• W2009152893 cites W2038139448 @default.
• W2009152893 cites W2048650744 @default.
• W2009152893 cites W2054979333 @default.
• W2009152893 cites W2068858849 @default.
• W2009152893 cites W2069425500 @default.
• W2009152893 cites W2074038681 @default.
• W2009152893 cites W2084449905 @default.
• W2009152893 cites W2094145772 @default.
• W2009152893 cites W2111301002 @default.
• W2009152893 cites W2122088836 @default.
• W2009152893 cites W2135238202 @default.
• W2009152893 cites W2500766147 @default.
• W2009152893 doi "https://doi.org/10.1016/j.foodchem.2003.08.009" @default.
• W2009152893 hasPublicationYear "2004" @default.
• W2009152893 type Work @default.
• W2009152893 sameAs 2009152893 @default.
• W2009152893 citedByCount "55" @default.
• W2009152893 countsByYear W20091528932012 @default.
• W2009152893 countsByYear W20091528932013 @default.
• W2009152893 countsByYear W20091528932014 @default.
• W2009152893 countsByYear W20091528932015 @default.
• W2009152893 countsByYear W20091528932016 @default.
• W2009152893 countsByYear W20091528932018 @default.
• W2009152893 countsByYear W20091528932019 @default.
• W2009152893 countsByYear W20091528932020 @default.
• W2009152893 countsByYear W20091528932021 @default.
• W2009152893 countsByYear W20091528932022 @default.
• W2009152893 countsByYear W20091528932023 @default.
• W2009152893 crossrefType "journal-article" @default.
• W2009152893 hasAuthorship W2009152893A5033197146 @default.
• W2009152893 hasAuthorship W2009152893A5037716507 @default.
• W2009152893 hasAuthorship W2009152893A5051833216 @default.
• W2009152893 hasAuthorship W2009152893A5089970167 @default.
• W2009152893 hasConcept C101660505 @default.
• W2009152893 hasConcept C116084860 @default.
• W2009152893 hasConcept C163994747 @default.
• W2009152893 hasConcept C178790620 @default.
• W2009152893 hasConcept C181199279 @default.
• W2009152893 hasConcept C185592680 @default.
• W2009152893 hasConcept C193363816 @default.
• W2009152893 hasConcept C197404232 @default.
• W2009152893 hasConcept C2778913249 @default.
• W2009152893 hasConcept C2778973965 @default.
• W2009152893 hasConcept C2779286987 @default.
• W2009152893 hasConcept C2779429693 @default.
• W2009152893 hasConcept C28212737 @default.
• W2009152893 hasConcept C2909208804 @default.
• W2009152893 hasConcept C43617362 @default.
• W2009152893 hasConcept C505870484 @default.
• W2009152893 hasConcept C55493867 @default.
• W2009152893 hasConcept C86803240 @default.
• W2009152893 hasConceptScore W2009152893C101660505 @default.
• W2009152893 hasConceptScore W2009152893C116084860 @default.
• W2009152893 hasConceptScore W2009152893C163994747 @default.
• W2009152893 hasConceptScore W2009152893C178790620 @default.
• W2009152893 hasConceptScore W2009152893C181199279 @default.
• W2009152893 hasConceptScore W2009152893C185592680 @default.
• W2009152893 hasConceptScore W2009152893C193363816 @default.
• W2009152893 hasConceptScore W2009152893C197404232 @default.
• W2009152893 hasConceptScore W2009152893C2778913249 @default.
• W2009152893 hasConceptScore W2009152893C2778973965 @default.
• W2009152893 hasConceptScore W2009152893C2779286987 @default.
• W2009152893 hasConceptScore W2009152893C2779429693 @default.
• W2009152893 hasConceptScore W2009152893C28212737 @default.
• W2009152893 hasConceptScore W2009152893C2909208804 @default.
• W2009152893 hasConceptScore W2009152893C43617362 @default.
• W2009152893 hasConceptScore W2009152893C505870484 @default.
• W2009152893 hasConceptScore W2009152893C55493867 @default.
• W2009152893 hasConceptScore W2009152893C86803240 @default.
• W2009152893 hasIssue "1" @default.
• W2009152893 hasLocation W20091528931 @default.
• W2009152893 hasOpenAccess W2009152893 @default.
• W2009152893 hasPrimaryLocation W20091528931 @default.
• W2009152893 hasRelatedWork W1560742689 @default.
• W2009152893 hasRelatedWork W1649084527 @default.
• W2009152893 hasRelatedWork W1954232397 @default.
• W2009152893 hasRelatedWork W1988452686 @default.
• W2009152893 hasRelatedWork W2001321907 @default.
• W2009152893 hasRelatedWork W2003005087 @default.
• W2009152893 hasRelatedWork W2029719858 @default.
• W2009152893 hasRelatedWork W2088905508 @default.
• W2009152893 hasRelatedWork W2146644869 @default.
• W2009152893 hasRelatedWork W2408980203 @default.
• W2009152893 hasVolume "86" @default.
• W2009152893 isParatext "false" @default.
• W2009152893 isRetracted "false" @default.

• next