FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009156976> ?p ?o ?g. }

• W2009156976 endingPage "1495" @default.
• W2009156976 startingPage "1485" @default.
• W2009156976 abstract "During adipogenic differentiation human mesenchymal stem cells (hMSC) produce collagen type IV. In immunofluorescence staining differentiating hMSCs started to express collagen type IV when Oil Red O-positive fat droplets appeared intracellularly. Quantitative real time-polymerase chain reaction confirmed progressive increase of collagen type IV α1 and α2 mRNA levels over time, 18.6- and 12.2-fold by day 28, respectively, whereas the copy numbers of α3-α6 mRNAs remained rather stable and low. Type IV collagen was in confocal laser scanning microscopy seen around adipocytes, where also laminins and nidogen were found, suggesting pericellular deposition of all key components of the fully developed basement membrane. Immunofluorescence staining of matrix metalloproteinase-2 (MMP-2, 72 kD type IV collagenase, gelatinase A) and MMP-9 (92 kD type IV collagenase, gelatinase B) disclosed only faint staining of MSCs, but MMP-9 was strongly induced during adipogenesis, whereas MSC supernatants disclosed in zymography pro-MMP-2 and faint pro-MMP-9 bands, which increased over time, with partial conversion of pro-MMP-2 to its active 62 kD form. Differentiation was associated with increasing membrane type 1-MMP/MMP-14 and tissue inhibitor of metalloproteinase-2 (TIMP-2) staining, which may enable participation of type IV collagenases in basement membrane remodelling via ternary MT1-MMP/TIMP-2/MMP-2 or -9 complexes, focalizing the fully active enzyme to the cell surface. MMP-9, which increased more in immunofluorescence staining, was perhaps preferentially bound to cell surface and/or remodelling adipocyte basement membrane. These results suggest that upon MSC-adipocyte differentiation collagen type IV synthesis and remodelling become necessary when intracellular accumulation of fat necessitates a dynamically supporting and instructive, partly denatured adipogenic pericellular type IV collagen scaffold." @default.
• W2009156976 created "2016-06-24" @default.
• W2009156976 creator A5000232949 @default.
• W2009156976 creator A5009137789 @default.
• W2009156976 creator A5025972377 @default.
• W2009156976 creator A5042103616 @default.
• W2009156976 creator A5050963625 @default.
• W2009156976 creator A5076012106 @default.
• W2009156976 date "2012-06-28" @default.
• W2009156976 modified "2023-10-14" @default.
• W2009156976 title "Basement membrane collagen type IV expression by human mesenchymal stem cells during adipogenic differentiation" @default.
• W2009156976 cites W1487090175 @default.
• W2009156976 cites W1488829562 @default.
• W2009156976 cites W1521876081 @default.
• W2009156976 cites W1927710162 @default.
• W2009156976 cites W1977390140 @default.
• W2009156976 cites W1979390430 @default.
• W2009156976 cites W1981193024 @default.
• W2009156976 cites W1991034520 @default.
• W2009156976 cites W1995669659 @default.
• W2009156976 cites W1997432717 @default.
• W2009156976 cites W2001514360 @default.
• W2009156976 cites W2016570094 @default.
• W2009156976 cites W2017778845 @default.
• W2009156976 cites W2018902817 @default.
• W2009156976 cites W2019210094 @default.
• W2009156976 cites W2019492370 @default.
• W2009156976 cites W2026542895 @default.
• W2009156976 cites W2030731213 @default.
• W2009156976 cites W2044615749 @default.
• W2009156976 cites W2049220062 @default.
• W2009156976 cites W2057958444 @default.
• W2009156976 cites W2064231568 @default.
• W2009156976 cites W2067401298 @default.
• W2009156976 cites W2084663547 @default.
• W2009156976 cites W2096252197 @default.
• W2009156976 cites W2101630029 @default.
• W2009156976 cites W2105220542 @default.
• W2009156976 cites W2108237388 @default.
• W2009156976 cites W2123371309 @default.
• W2009156976 cites W2125932935 @default.
• W2009156976 cites W2144268541 @default.
• W2009156976 cites W2148191159 @default.
• W2009156976 cites W2152459643 @default.
• W2009156976 cites W2153147060 @default.
• W2009156976 cites W2154558576 @default.
• W2009156976 cites W2159999899 @default.
• W2009156976 cites W4236861583 @default.
• W2009156976 doi "https://doi.org/10.1111/j.1582-4934.2011.01442.x" @default.
• W2009156976 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3823217" @default.
• W2009156976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21883898" @default.
• W2009156976 hasPublicationYear "2012" @default.
• W2009156976 type Work @default.
• W2009156976 sameAs 2009156976 @default.
• W2009156976 citedByCount "54" @default.
• W2009156976 countsByYear W20091569762012 @default.
• W2009156976 countsByYear W20091569762013 @default.
• W2009156976 countsByYear W20091569762014 @default.
• W2009156976 countsByYear W20091569762015 @default.
• W2009156976 countsByYear W20091569762016 @default.
• W2009156976 countsByYear W20091569762017 @default.
• W2009156976 countsByYear W20091569762018 @default.
• W2009156976 countsByYear W20091569762019 @default.
• W2009156976 countsByYear W20091569762020 @default.
• W2009156976 countsByYear W20091569762021 @default.
• W2009156976 countsByYear W20091569762022 @default.
• W2009156976 countsByYear W20091569762023 @default.
• W2009156976 crossrefType "journal-article" @default.
• W2009156976 hasAuthorship W2009156976A5000232949 @default.
• W2009156976 hasAuthorship W2009156976A5009137789 @default.
• W2009156976 hasAuthorship W2009156976A5025972377 @default.
• W2009156976 hasAuthorship W2009156976A5042103616 @default.
• W2009156976 hasAuthorship W2009156976A5050963625 @default.
• W2009156976 hasAuthorship W2009156976A5076012106 @default.
• W2009156976 hasBestOaLocation W20091569761 @default.
• W2009156976 hasConcept C109523444 @default.
• W2009156976 hasConcept C134018914 @default.
• W2009156976 hasConcept C153911025 @default.
• W2009156976 hasConcept C181199279 @default.
• W2009156976 hasConcept C185592680 @default.
• W2009156976 hasConcept C189165786 @default.
• W2009156976 hasConcept C198826908 @default.
• W2009156976 hasConcept C2776193487 @default.
• W2009156976 hasConcept C2776223131 @default.
• W2009156976 hasConcept C2776232661 @default.
• W2009156976 hasConcept C2777107211 @default.
• W2009156976 hasConcept C2778394429 @default.
• W2009156976 hasConcept C2779814568 @default.
• W2009156976 hasConcept C2780644872 @default.
• W2009156976 hasConcept C54355233 @default.
• W2009156976 hasConcept C55493867 @default.
• W2009156976 hasConcept C74864618 @default.
• W2009156976 hasConcept C86803240 @default.
• W2009156976 hasConcept C95444343 @default.
• W2009156976 hasConceptScore W2009156976C109523444 @default.
• W2009156976 hasConceptScore W2009156976C134018914 @default.
• W2009156976 hasConceptScore W2009156976C153911025 @default.
• W2009156976 hasConceptScore W2009156976C181199279 @default.

• next