FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009159272> ?p ?o ?g. }

• W2009159272 endingPage "1437" @default.
• W2009159272 startingPage "1433" @default.
• W2009159272 abstract "Metalloproteases from at least two Gram-negative organisms selectively hydrolyze the surface of human fixed washed platelets. The protease-treated platelets lose their ability to aggregate with bovine von Willebrand factor and human von Willebrand factor plus ristocetin. The present study reports the membrane glycoprotein alterations and loss of platelet function that occur after incubation of fresh washed human platelets with a purified protease produced by Serratia marcescens. The studies were undertaken in order to examine, using the Serratia protease as an enzymatic probe, the relationship between externally oriented glycoproteins and two known aggregating agents, bovine von Willebrand factor and thrombin. Platelet membrane glycoproteins were analyzed after discontinuous NaDodSO(4)/polyacrylamide gel electrophoresis using both staining with periodic acid-Schiff reagent and autoradiography of (3)H-labeled platelets. Both methods of detection demonstrated that at Serratia protease concentrations above 0.6 mug/ml there was hydrolysis within 3 min of a membrane glycoprotein (M(r) 185,000) corresponding to GPIb in control platelets. The loss of GPIb was accompanied by the appearance in the platelet supernatant of a glycopeptide (M(r) 156,000). Under reducing conditions, the hydrolyzed membrane glycopeptide in the supernatant had a consistently faster migration (M(r) 149,000). Platelets treated with Serratia protease at concentrations sufficient to give maximal cleavage of GPIb were unresponsive to bovine von Willebrand factor and did not aggregate or release [(14)C]serotonin at doses of von Willebrand factor up to 0.33 unit/ml. On the other hand, the response to bovine alpha-thrombin was only minimally impaired by treatment with Serratia protease. These results implicate GPIb in the response by platelets to bovine von Willebrand factor but suggest that surface components other than GPIb play a major role in the response by platelets to thrombin." @default.
• W2009159272 created "2016-06-24" @default.
• W2009159272 creator A5009233585 @default.
• W2009159272 creator A5019490847 @default.
• W2009159272 creator A5044416046 @default.
• W2009159272 creator A5071472635 @default.
• W2009159272 date "1982-03-01" @default.
• W2009159272 modified "2023-09-23" @default.
• W2009159272 title "Hydrolysis of human platelet membrane glycoproteins with a <i>Serratia marcescens</i> metalloprotease: Effect on response to thrombin and von Willebrand factor" @default.
• W2009159272 cites W123749191 @default.
• W2009159272 cites W1520399899 @default.
• W2009159272 cites W1568858788 @default.
• W2009159272 cites W1570929890 @default.
• W2009159272 cites W1575000195 @default.
• W2009159272 cites W1967324116 @default.
• W2009159272 cites W1970835250 @default.
• W2009159272 cites W1991848195 @default.
• W2009159272 cites W2006799862 @default.
• W2009159272 cites W2009763777 @default.
• W2009159272 cites W2018289835 @default.
• W2009159272 cites W2023607502 @default.
• W2009159272 cites W2026146058 @default.
• W2009159272 cites W2034277598 @default.
• W2009159272 cites W2046361278 @default.
• W2009159272 cites W2049692254 @default.
• W2009159272 cites W2052094977 @default.
• W2009159272 cites W2054513638 @default.
• W2009159272 cites W2074011110 @default.
• W2009159272 cites W2100837269 @default.
• W2009159272 cites W2168110441 @default.
• W2009159272 cites W2397770649 @default.
• W2009159272 cites W2400528759 @default.
• W2009159272 cites W2409387175 @default.
• W2009159272 cites W2413105515 @default.
• W2009159272 cites W2443070490 @default.
• W2009159272 cites W31270972 @default.
• W2009159272 doi "https://doi.org/10.1073/pnas.79.5.1433" @default.
• W2009159272 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/345987" @default.
• W2009159272 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7041120" @default.
• W2009159272 hasPublicationYear "1982" @default.
• W2009159272 type Work @default.
• W2009159272 sameAs 2009159272 @default.
• W2009159272 citedByCount "36" @default.
• W2009159272 countsByYear W20091592722017 @default.
• W2009159272 crossrefType "journal-article" @default.
• W2009159272 hasAuthorship W2009159272A5009233585 @default.
• W2009159272 hasAuthorship W2009159272A5019490847 @default.
• W2009159272 hasAuthorship W2009159272A5044416046 @default.
• W2009159272 hasAuthorship W2009159272A5071472635 @default.
• W2009159272 hasBestOaLocation W20091592721 @default.
• W2009159272 hasConcept C104317684 @default.
• W2009159272 hasConcept C108625454 @default.
• W2009159272 hasConcept C116084860 @default.
• W2009159272 hasConcept C153911025 @default.
• W2009159272 hasConcept C181199279 @default.
• W2009159272 hasConcept C185592680 @default.
• W2009159272 hasConcept C203014093 @default.
• W2009159272 hasConcept C26828662 @default.
• W2009159272 hasConcept C2776057759 @default.
• W2009159272 hasConcept C2776309202 @default.
• W2009159272 hasConcept C2776714187 @default.
• W2009159272 hasConcept C2777292125 @default.
• W2009159272 hasConcept C2778442980 @default.
• W2009159272 hasConcept C2779394231 @default.
• W2009159272 hasConcept C547475151 @default.
• W2009159272 hasConcept C55493867 @default.
• W2009159272 hasConcept C86803240 @default.
• W2009159272 hasConcept C89560881 @default.
• W2009159272 hasConceptScore W2009159272C104317684 @default.
• W2009159272 hasConceptScore W2009159272C108625454 @default.
• W2009159272 hasConceptScore W2009159272C116084860 @default.
• W2009159272 hasConceptScore W2009159272C153911025 @default.
• W2009159272 hasConceptScore W2009159272C181199279 @default.
• W2009159272 hasConceptScore W2009159272C185592680 @default.
• W2009159272 hasConceptScore W2009159272C203014093 @default.
• W2009159272 hasConceptScore W2009159272C26828662 @default.
• W2009159272 hasConceptScore W2009159272C2776057759 @default.
• W2009159272 hasConceptScore W2009159272C2776309202 @default.
• W2009159272 hasConceptScore W2009159272C2776714187 @default.
• W2009159272 hasConceptScore W2009159272C2777292125 @default.
• W2009159272 hasConceptScore W2009159272C2778442980 @default.
• W2009159272 hasConceptScore W2009159272C2779394231 @default.
• W2009159272 hasConceptScore W2009159272C547475151 @default.
• W2009159272 hasConceptScore W2009159272C55493867 @default.
• W2009159272 hasConceptScore W2009159272C86803240 @default.
• W2009159272 hasConceptScore W2009159272C89560881 @default.
• W2009159272 hasIssue "5" @default.
• W2009159272 hasLocation W20091592721 @default.
• W2009159272 hasLocation W20091592722 @default.
• W2009159272 hasLocation W20091592723 @default.
• W2009159272 hasLocation W20091592724 @default.
• W2009159272 hasLocation W20091592725 @default.
• W2009159272 hasOpenAccess W2009159272 @default.
• W2009159272 hasPrimaryLocation W20091592721 @default.
• W2009159272 hasRelatedWork W1227255805 @default.
• W2009159272 hasRelatedWork W144290400 @default.
• W2009159272 hasRelatedWork W1992646081 @default.
• W2009159272 hasRelatedWork W2084785484 @default.

• next