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- W2009215649 abstract "Many important applications of DNA sequence-dependent hybridization reactions have recently emerged. This has sparked a renewed interest in analytical calculations of sequence-dependent melting stability of duplex DNA. In particular, for many applications it is often desirable to accurately predict the transition temperature, or tm of short duplex DNA oligomers (approximately 20 base pairs or less) from their sequence and concentration. The thermodynamic analytical method underlying these predictive calculations is based on the nearest-neighbor model. At least 11 sets of nearest-neighbor sequence-dependent thermodynamic parameters for DNA have been published. These sets are compared. Use of the nearest-neighbor sets in predicting tm from the DNA sequence is demonstrated, and the ability of the nearest-neighbor parameters to provide accurate predictions of experimental tm's of short duplex DNA oligomers is assessed." @default.
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- W2009215649 date "1997-01-01" @default.
- W2009215649 modified "2023-10-15" @default.
- W2009215649 title "Predicting sequence-dependent melting stability of short duplex DNA oligomers" @default.
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- W2009215649 doi "https://doi.org/10.1002/(sici)1097-0282(1997)44:3<217::aid-bip3>3.0.co;2-y" @default.
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