FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009253350> ?p ?o ?g. }

• W2009253350 endingPage "e1468" @default.
• W2009253350 startingPage "e1468" @default.
• W2009253350 abstract "Background Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle. Over the past ten years, microfluidic techniques in cell biology have emerged that allow for good control of growth environment. Yet the control and quantification of transient gene expression in unperturbed dividing cells has received less attention. Methodology/Principal Findings Here, we describe a microfluidic flow cell to grow Saccharomyces Cerevisiae for more than 8 generations (≈12 hrs) starting with single cells, with controlled flow of the growth medium. This setup provides two important features: first, cells are tightly confined and grow in a remarkably planar array. The pedigree can thus be determined and single-cell fluorescence measured with 3 minutes resolution for all cells, as a founder cell grows to a micro-colony of more than 200 cells. Second, we can trigger and calibrate rapid and transient gene expression using reversible administration of inducers that control the GAL1 or MET3 promoters. We then show that periodic 10–20 minutes gene induction pulses can drive many cell division cycles with complete coherence across the cell cluster, with either a G1/S trigger (cln1 cln2 cln3 MET3-CLN2) or a mitotic trigger (cdc20 GALL-CDC20). Conclusions/Significance In addition to evident cell cycle applications, this device can be used to directly measure the amount and duration of any fluorescently scorable signal-transduction or gene-induction response over a long time period. The system allows direct correlation of cell history (e.g., hysteresis or epigenetics) or cell cycle position with the measured response." @default.
• W2009253350 created "2016-06-24" @default.
• W2009253350 creator A5036408112 @default.
• W2009253350 creator A5061209953 @default.
• W2009253350 creator A5083225830 @default.
• W2009253350 date "2008-01-23" @default.
• W2009253350 modified "2023-10-17" @default.
• W2009253350 title "A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells" @default.
• W2009253350 cites W1590458072 @default.
• W2009253350 cites W1979420899 @default.
• W2009253350 cites W2000456081 @default.
• W2009253350 cites W2015038174 @default.
• W2009253350 cites W2029361184 @default.
• W2009253350 cites W2035230904 @default.
• W2009253350 cites W2039210066 @default.
• W2009253350 cites W2039808072 @default.
• W2009253350 cites W2041797933 @default.
• W2009253350 cites W2047076849 @default.
• W2009253350 cites W2047300775 @default.
• W2009253350 cites W2063856790 @default.
• W2009253350 cites W2078285432 @default.
• W2009253350 cites W2081107232 @default.
• W2009253350 cites W2081298797 @default.
• W2009253350 cites W2098941196 @default.
• W2009253350 cites W2100866112 @default.
• W2009253350 cites W2103453943 @default.
• W2009253350 cites W2124918094 @default.
• W2009253350 cites W2127068865 @default.
• W2009253350 cites W2128104520 @default.
• W2009253350 cites W2148323109 @default.
• W2009253350 cites W2169805130 @default.
• W2009253350 cites W2171416668 @default.
• W2009253350 cites W2188142152 @default.
• W2009253350 doi "https://doi.org/10.1371/journal.pone.0001468" @default.
• W2009253350 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2194624" @default.
• W2009253350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18213377" @default.
• W2009253350 hasPublicationYear "2008" @default.
• W2009253350 type Work @default.
• W2009253350 sameAs 2009253350 @default.
• W2009253350 citedByCount "126" @default.
• W2009253350 countsByYear W20092533502012 @default.
• W2009253350 countsByYear W20092533502013 @default.
• W2009253350 countsByYear W20092533502014 @default.
• W2009253350 countsByYear W20092533502015 @default.
• W2009253350 countsByYear W20092533502016 @default.
• W2009253350 countsByYear W20092533502017 @default.
• W2009253350 countsByYear W20092533502018 @default.
• W2009253350 countsByYear W20092533502019 @default.
• W2009253350 countsByYear W20092533502020 @default.
• W2009253350 countsByYear W20092533502021 @default.
• W2009253350 countsByYear W20092533502022 @default.
• W2009253350 countsByYear W20092533502023 @default.
• W2009253350 crossrefType "journal-article" @default.
• W2009253350 hasAuthorship W2009253350A5036408112 @default.
• W2009253350 hasAuthorship W2009253350A5061209953 @default.
• W2009253350 hasAuthorship W2009253350A5083225830 @default.
• W2009253350 hasBestOaLocation W20092533501 @default.
• W2009253350 hasConcept C104317684 @default.
• W2009253350 hasConcept C1491633281 @default.
• W2009253350 hasConcept C150194340 @default.
• W2009253350 hasConcept C15526883 @default.
• W2009253350 hasConcept C2776950831 @default.
• W2009253350 hasConcept C2777576037 @default.
• W2009253350 hasConcept C29452124 @default.
• W2009253350 hasConcept C29537977 @default.
• W2009253350 hasConcept C54355233 @default.
• W2009253350 hasConcept C62112901 @default.
• W2009253350 hasConcept C85813293 @default.
• W2009253350 hasConcept C86803240 @default.
• W2009253350 hasConcept C93304396 @default.
• W2009253350 hasConcept C95444343 @default.
• W2009253350 hasConceptScore W2009253350C104317684 @default.
• W2009253350 hasConceptScore W2009253350C1491633281 @default.
• W2009253350 hasConceptScore W2009253350C150194340 @default.
• W2009253350 hasConceptScore W2009253350C15526883 @default.
• W2009253350 hasConceptScore W2009253350C2776950831 @default.
• W2009253350 hasConceptScore W2009253350C2777576037 @default.
• W2009253350 hasConceptScore W2009253350C29452124 @default.
• W2009253350 hasConceptScore W2009253350C29537977 @default.
• W2009253350 hasConceptScore W2009253350C54355233 @default.
• W2009253350 hasConceptScore W2009253350C62112901 @default.
• W2009253350 hasConceptScore W2009253350C85813293 @default.
• W2009253350 hasConceptScore W2009253350C86803240 @default.
• W2009253350 hasConceptScore W2009253350C93304396 @default.
• W2009253350 hasConceptScore W2009253350C95444343 @default.
• W2009253350 hasIssue "1" @default.
• W2009253350 hasLocation W20092533501 @default.
• W2009253350 hasLocation W20092533502 @default.
• W2009253350 hasLocation W20092533503 @default.
• W2009253350 hasLocation W20092533504 @default.
• W2009253350 hasLocation W20092533505 @default.
• W2009253350 hasLocation W20092533506 @default.
• W2009253350 hasLocation W20092533507 @default.
• W2009253350 hasLocation W20092533508 @default.
• W2009253350 hasOpenAccess W2009253350 @default.
• W2009253350 hasPrimaryLocation W20092533501 @default.
• W2009253350 hasRelatedWork W1576499812 @default.
• W2009253350 hasRelatedWork W1659509344 @default.

• next