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- W2009333663 abstract "Abstract An enzymic method for producing a disaccharide was developed using the endo-α-N-acetylgalactosaminidase from Alcaligenes sp. From 0.5 g asialofetuin, 2.66 mg of pure disaccharide was obtained by procedures including enzymic hydrolysis, alcohol fractionation, gel filtration on Sephadex G-10 and normal-phase high-performance liquid chromatography on Cosmosil 5 NH2. The structure of the disaccharide was determined as Ga1β1→3GalNAc by thin-layer chromatography, gas-liquid chromatography and proton nuclear magnetic resonance spectroscopy. The molecular extinction coefficients of the disaccharide at 210 nm, 200 nm and 190 nm were found to be 1.17 × 103 M−1, 4.36 × 103 M−1 and 8.00 × 103 M−1, respectively, and the molecular extinction coefficient of its derivative at 585 nm was determined as 15.6 × 103 M−1 by using the method of Reissig et al (J. Biol. Chem., 217, 959–966, 1955). This method of producing the disaccharide is considered to be applicable for practical use." @default.
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- W2009333663 date "1991-01-01" @default.
- W2009333663 modified "2023-09-22" @default.
- W2009333663 title "Unique production of a disaccharide, Ga1β1→3GalNAc, by endo-α-N-acetylgalactosaminidase of Alcaligenes sp." @default.
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- W2009333663 doi "https://doi.org/10.1016/0922-338x(91)90315-8" @default.
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