FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009335979> ?p ?o ?g. }

• W2009335979 endingPage "9225" @default.
• W2009335979 startingPage "9216" @default.
• W2009335979 abstract "Lipopolysaccharide (LPS; endotoxin) is an essential component of the outer monolayer of nearly all Gram-negative bacteria. LPS is composed of a hydrophobic anchor, known as lipid A, an inner core oligosaccharide, and a repeating O-antigen polysaccharide. In nearly all species, the first sugar bridging the hydrophobic lipid A and the polysaccharide domain is 3-deoxy-d-manno-octulosonic acid (Kdo), and thus it is critically important for LPS biosynthesis. Modifications to lipid A have been shown to be important for resistance to antimicrobial peptides as well as modulating recognition by the mammalian innate immune system. Therefore, lipid A derivatives have been used for development of vaccine strains and vaccine adjuvants. One derivative that has yet to be studied is 8-amino-3,8-dideoxy-d-manno-octulosonic acid (Kdo8N), which is found exclusively in marine bacteria of the genus Shewanella. Using bioinformatics, a candidate gene cluster for Kdo8N biosynthesis was identified in Shewanella oneidensis. Expression of these genes recombinantly in Escherichia coli resulted in lipid A containing Kdo8N, and in vitro assays confirmed their proposed enzymatic function. Both the in vivo and in vitro data were consistent with direct conversion of Kdo to Kdo8N prior to its incorporation into the Kdo8N-lipid A domain of LPS by a metal-dependent oxidase followed by a glutamate-dependent aminotransferase. To our knowledge, this oxidase is the first enzyme shown to oxidize an alcohol using a metal and molecular oxygen, not NAD(P)+. Creation of an S. oneidensis in-frame deletion strain showed increased sensitivity to the cationic antimicrobial peptide polymyxin as well as bile salts, suggesting a role in outer membrane integrity. Lipopolysaccharide (LPS; endotoxin) is an essential component of the outer monolayer of nearly all Gram-negative bacteria. LPS is composed of a hydrophobic anchor, known as lipid A, an inner core oligosaccharide, and a repeating O-antigen polysaccharide. In nearly all species, the first sugar bridging the hydrophobic lipid A and the polysaccharide domain is 3-deoxy-d-manno-octulosonic acid (Kdo), and thus it is critically important for LPS biosynthesis. Modifications to lipid A have been shown to be important for resistance to antimicrobial peptides as well as modulating recognition by the mammalian innate immune system. Therefore, lipid A derivatives have been used for development of vaccine strains and vaccine adjuvants. One derivative that has yet to be studied is 8-amino-3,8-dideoxy-d-manno-octulosonic acid (Kdo8N), which is found exclusively in marine bacteria of the genus Shewanella. Using bioinformatics, a candidate gene cluster for Kdo8N biosynthesis was identified in Shewanella oneidensis. Expression of these genes recombinantly in Escherichia coli resulted in lipid A containing Kdo8N, and in vitro assays confirmed their proposed enzymatic function. Both the in vivo and in vitro data were consistent with direct conversion of Kdo to Kdo8N prior to its incorporation into the Kdo8N-lipid A domain of LPS by a metal-dependent oxidase followed by a glutamate-dependent aminotransferase. To our knowledge, this oxidase is the first enzyme shown to oxidize an alcohol using a metal and molecular oxygen, not NAD(P)+. Creation of an S. oneidensis in-frame deletion strain showed increased sensitivity to the cationic antimicrobial peptide polymyxin as well as bile salts, suggesting a role in outer membrane integrity." @default.
• W2009335979 created "2016-06-24" @default.
• W2009335979 creator A5010161614 @default.
• W2009335979 creator A5043774968 @default.
• W2009335979 creator A5073031989 @default.
• W2009335979 creator A5088119780 @default.
• W2009335979 date "2013-03-01" @default.
• W2009335979 modified "2023-10-15" @default.
• W2009335979 title "The Origin of 8-Amino-3,8-dideoxy-d-manno-octulosonic Acid (Kdo8N) in the Lipopolysaccharide of Shewanella oneidensis" @default.
• W2009335979 cites W1557228498 @default.
• W2009335979 cites W1636002209 @default.
• W2009335979 cites W1965270386 @default.
• W2009335979 cites W1967398671 @default.
• W2009335979 cites W1971425188 @default.
• W2009335979 cites W1974127479 @default.
• W2009335979 cites W1975955304 @default.
• W2009335979 cites W2003845371 @default.
• W2009335979 cites W2004589024 @default.
• W2009335979 cites W2010435105 @default.
• W2009335979 cites W2014611308 @default.
• W2009335979 cites W2037993016 @default.
• W2009335979 cites W2054941036 @default.
• W2009335979 cites W2073553628 @default.
• W2009335979 cites W2079653018 @default.
• W2009335979 cites W2082699708 @default.
• W2009335979 cites W2092278008 @default.
• W2009335979 cites W2094414498 @default.
• W2009335979 cites W2101871915 @default.
• W2009335979 cites W2108649941 @default.
• W2009335979 cites W2108921340 @default.
• W2009335979 cites W2113259665 @default.
• W2009335979 cites W2123783604 @default.
• W2009335979 cites W2132200710 @default.
• W2009335979 cites W2135707422 @default.
• W2009335979 cites W2146309582 @default.
• W2009335979 cites W2171780940 @default.
• W2009335979 cites W2300552860 @default.
• W2009335979 cites W3166530837 @default.
• W2009335979 cites W4234317413 @default.
• W2009335979 doi "https://doi.org/10.1074/jbc.m113.453324" @default.
• W2009335979 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3610993" @default.
• W2009335979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23413030" @default.
• W2009335979 hasPublicationYear "2013" @default.
• W2009335979 type Work @default.
• W2009335979 sameAs 2009335979 @default.
• W2009335979 citedByCount "15" @default.
• W2009335979 countsByYear W20093359792013 @default.
• W2009335979 countsByYear W20093359792014 @default.
• W2009335979 countsByYear W20093359792015 @default.
• W2009335979 countsByYear W20093359792016 @default.
• W2009335979 countsByYear W20093359792017 @default.
• W2009335979 countsByYear W20093359792018 @default.
• W2009335979 countsByYear W20093359792019 @default.
• W2009335979 crossrefType "journal-article" @default.
• W2009335979 hasAuthorship W2009335979A5010161614 @default.
• W2009335979 hasAuthorship W2009335979A5043774968 @default.
• W2009335979 hasAuthorship W2009335979A5073031989 @default.
• W2009335979 hasAuthorship W2009335979A5088119780 @default.
• W2009335979 hasBestOaLocation W20093359791 @default.
• W2009335979 hasConcept C104317684 @default.
• W2009335979 hasConcept C134018914 @default.
• W2009335979 hasConcept C144464004 @default.
• W2009335979 hasConcept C146587185 @default.
• W2009335979 hasConcept C181199279 @default.
• W2009335979 hasConcept C185592680 @default.
• W2009335979 hasConcept C2777108408 @default.
• W2009335979 hasConcept C2778754761 @default.
• W2009335979 hasConcept C2778824058 @default.
• W2009335979 hasConcept C2781170380 @default.
• W2009335979 hasConcept C2910339780 @default.
• W2009335979 hasConcept C38485361 @default.
• W2009335979 hasConcept C523546767 @default.
• W2009335979 hasConcept C54355233 @default.
• W2009335979 hasConcept C547475151 @default.
• W2009335979 hasConcept C55493867 @default.
• W2009335979 hasConcept C86803240 @default.
• W2009335979 hasConcept C89423630 @default.
• W2009335979 hasConceptScore W2009335979C104317684 @default.
• W2009335979 hasConceptScore W2009335979C134018914 @default.
• W2009335979 hasConceptScore W2009335979C144464004 @default.
• W2009335979 hasConceptScore W2009335979C146587185 @default.
• W2009335979 hasConceptScore W2009335979C181199279 @default.
• W2009335979 hasConceptScore W2009335979C185592680 @default.
• W2009335979 hasConceptScore W2009335979C2777108408 @default.
• W2009335979 hasConceptScore W2009335979C2778754761 @default.
• W2009335979 hasConceptScore W2009335979C2778824058 @default.
• W2009335979 hasConceptScore W2009335979C2781170380 @default.
• W2009335979 hasConceptScore W2009335979C2910339780 @default.
• W2009335979 hasConceptScore W2009335979C38485361 @default.
• W2009335979 hasConceptScore W2009335979C523546767 @default.
• W2009335979 hasConceptScore W2009335979C54355233 @default.
• W2009335979 hasConceptScore W2009335979C547475151 @default.
• W2009335979 hasConceptScore W2009335979C55493867 @default.
• W2009335979 hasConceptScore W2009335979C86803240 @default.
• W2009335979 hasConceptScore W2009335979C89423630 @default.
• W2009335979 hasIssue "13" @default.
• W2009335979 hasLocation W20093359791 @default.
• W2009335979 hasLocation W20093359792 @default.

• next