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- W2009348112 abstract "DNA content in tumor cells from 50 patients with ovarian tumors was analysed by flow cytometry (FCM). Solid tissue samples were processed to obtain monodispersed cells. Staining for DNA analysis was achieved with ethidium bromide and mithramycin. Peripheral blood lymphocytes were used as reference diploid cell population. All benign ovarian tumors exhibited only diploid cells. DNA aneuploid cell lines were found 66.6% of serous carcinomas and in 80% of malignant granulosa cell tumors. The S-phase fraction of DNA diploid cells in benign ovarian tumors (S = 2.4 ± 1.2%) was smaller than those of malignant tumors (S = 8.2 ± 5.2%). DNA aneuploid cell populations in serous carcinomas display a higher S-phase fraction (S = 19.2 ± 9.3%) than DNA diploid cells (S = 11.7 ± 3.2%). No major differences were obtained between primary ovarian tumors and their metastases, as far as degree of aneuploidy and S-phase fraction are concerned. A high degree of correlation was established between the grade of differentiation of ovarian tumors and the DNA ploidy abnormalities." @default.
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- W2009348112 title "Flow cytometric analysis of DNA and cell proliferation in ovarian tumors" @default.
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- W2009348112 doi "https://doi.org/10.1002/1097-0142(19880101)61:1<121::aid-cncr2820610121>3.0.co;2-n" @default.
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