FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009353679> ?p ?o ?g. }

• W2009353679 endingPage "69" @default.
• W2009353679 startingPage "56" @default.
• W2009353679 abstract "A comprehensive proteomic profiling of nasal epithelium (NE) is described. This study relies on simple subcellular fractionation used to obtain soluble- and membrane-enriched fractions followed by 2-dimensional liquid chromatography (2D-LC) separation and tandem mass spectrometry (MS/MS). The cells were collected using a brushing technique applied on NE of clinically evaluated volunteers. Subsequently, the soluble- and the membrane-protein enriched fractions were prepared and analyzed in parallel using 2D-LC-MS/MS. In a set of 1482 identified proteins, 947 (63.9%) proteins were found to be associated to membrane fraction. Grand average hydropathy value index (GRAVY) analysis, the transmembrane protein mapping and annotations of primary location deposited in the Human Protein Reference Database (HPRD) confirmed an enrichment of hydrophobic proteins on this dataset. Ingenuity Pathway Analysis (IPA) of soluble fraction revealed an enrichment of molecular and cellular functions associated with cell death, protein folding and drug metabolism while in membrane fraction showed an enrichment of functions associated with molecular transport, protein trafficking and cell-to-cell signaling and interaction. The IPA showed similar enrichment of functions associated with cellular growth and proliferation in both soluble and membrane subproteomes. This finding was in agreement with protein content analysis using exponentially modified protein abundance index (emPAI). A comparison of our data with previously published studies focusing on respiratory tract epithelium revealed similarities related to identification of proteins associated with physical barrier function and immunological defence. In summary, we extended the NE molecular profile by identifying and characterizing proteins associated to pivotal functions of a respiratory epithelium, including the control of fluid volume and ionic composition at the airways' surface, physical barrier maintenance, detoxification and immunological defence. The extent of similarities supports the applicability of a less invasive analysis of NE to assess prognosis and treatment response of lung diseases such as asthma, cystic fibrosis and chronic obstructive pulmonary disease." @default.
• W2009353679 created "2016-06-24" @default.
• W2009353679 creator A5009766837 @default.
• W2009353679 creator A5014333753 @default.
• W2009353679 creator A5017124618 @default.
• W2009353679 creator A5019010663 @default.
• W2009353679 creator A5024999170 @default.
• W2009353679 creator A5030805150 @default.
• W2009353679 creator A5039171289 @default.
• W2009353679 creator A5049463180 @default.
• W2009353679 creator A5071552003 @default.
• W2009353679 creator A5072979086 @default.
• W2009353679 date "2011-12-01" @default.
• W2009353679 modified "2023-10-01" @default.
• W2009353679 title "Molecular profiling of the human nasal epithelium: A proteomics approach" @default.
• W2009353679 cites W1528658828 @default.
• W2009353679 cites W1951204040 @default.
• W2009353679 cites W1968261842 @default.
• W2009353679 cites W1975304761 @default.
• W2009353679 cites W1976158279 @default.
• W2009353679 cites W1979170909 @default.
• W2009353679 cites W1984492870 @default.
• W2009353679 cites W1985504702 @default.
• W2009353679 cites W1986713362 @default.
• W2009353679 cites W1986737545 @default.
• W2009353679 cites W1988179473 @default.
• W2009353679 cites W1991265255 @default.
• W2009353679 cites W1991975049 @default.
• W2009353679 cites W1996817596 @default.
• W2009353679 cites W1999274391 @default.
• W2009353679 cites W2000641235 @default.
• W2009353679 cites W2010781630 @default.
• W2009353679 cites W2012913476 @default.
• W2009353679 cites W2013711061 @default.
• W2009353679 cites W2014261553 @default.
• W2009353679 cites W2015392958 @default.
• W2009353679 cites W201601905 @default.
• W2009353679 cites W2016087958 @default.
• W2009353679 cites W2019115288 @default.
• W2009353679 cites W2023207330 @default.
• W2009353679 cites W2029078364 @default.
• W2009353679 cites W2030600480 @default.
• W2009353679 cites W2030727312 @default.
• W2009353679 cites W2031349943 @default.
• W2009353679 cites W2036562750 @default.
• W2009353679 cites W2040233068 @default.
• W2009353679 cites W2041718910 @default.
• W2009353679 cites W2042151879 @default.
• W2009353679 cites W2045154814 @default.
• W2009353679 cites W2048237256 @default.
• W2009353679 cites W2053315960 @default.
• W2009353679 cites W2053735968 @default.
• W2009353679 cites W2057761610 @default.
• W2009353679 cites W2061853352 @default.
• W2009353679 cites W2062604348 @default.
• W2009353679 cites W2063575573 @default.
• W2009353679 cites W2064579441 @default.
• W2009353679 cites W2065220920 @default.
• W2009353679 cites W2066768613 @default.
• W2009353679 cites W2069838626 @default.
• W2009353679 cites W2071725693 @default.
• W2009353679 cites W2072662834 @default.
• W2009353679 cites W2073411380 @default.
• W2009353679 cites W2073526291 @default.
• W2009353679 cites W2082438874 @default.
• W2009353679 cites W2089820399 @default.
• W2009353679 cites W2096000363 @default.
• W2009353679 cites W2096154351 @default.
• W2009353679 cites W2098979213 @default.
• W2009353679 cites W2104196253 @default.
• W2009353679 cites W2107367586 @default.
• W2009353679 cites W2109814336 @default.
• W2009353679 cites W2111220371 @default.
• W2009353679 cites W2112180503 @default.
• W2009353679 cites W2112299827 @default.
• W2009353679 cites W2112742649 @default.
• W2009353679 cites W2120685260 @default.
• W2009353679 cites W2122755362 @default.
• W2009353679 cites W2127822814 @default.
• W2009353679 cites W2129617994 @default.
• W2009353679 cites W2136412900 @default.
• W2009353679 cites W2140880010 @default.
• W2009353679 cites W2141430390 @default.
• W2009353679 cites W2147045884 @default.
• W2009353679 cites W2147686705 @default.
• W2009353679 cites W2148490163 @default.
• W2009353679 cites W2149738087 @default.
• W2009353679 cites W2154261437 @default.
• W2009353679 cites W2157963288 @default.
• W2009353679 cites W2168627573 @default.
• W2009353679 cites W2415186660 @default.
• W2009353679 doi "https://doi.org/10.1016/j.jprot.2011.05.012" @default.
• W2009353679 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7185466" @default.
• W2009353679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21621024" @default.
• W2009353679 hasPublicationYear "2011" @default.
• W2009353679 type Work @default.
• W2009353679 sameAs 2009353679 @default.
• W2009353679 citedByCount "17" @default.

• next