Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009363604> ?p ?o ?g. }
- W2009363604 endingPage "974" @default.
- W2009363604 startingPage "967" @default.
- W2009363604 abstract "Alterations of the vessel structure, which is mainly determined by smooth muscle cells through cell growth and/or cell death mechanisms, are characteristic of diabetes complications. We analysed the influence of high glucose (22 m M ) on cultured human aortic smooth muscle cell growth and death, as hyperglycaemia is considered one of the main factors involved in diabetic vasculopathy. Growth curves were performed over 96 h in medium containing 0.5% foetal calf serum. Cell number increased by 2–4 fold over the culture period in the presence of 5.5 m M (low) glucose, while a 20% reduction in final cell number was observed with high glucose. Under serum‐free conditions, cell number remained constant in low glucose cultures, but a 40% decrease was observed in high glucose cultures, suggesting that high glucose may induce increased cell death rather than reduced proliferation. Reduced final cell number induced by high glucose was also observed after stimulation with 5 or 10% foetal calf serum. The possible participation of oxidative stress was investigated by co‐incubating high glucose with different reactive oxygen species scavengers. Only catalase reversed the effect of high glucose. Intracellular H 2 O 2 content, visualized with 2′,7′‐dichlorofluorescein and quantified by flow cytometry, was increased after high glucose treatment. To investigate the cell death mechanism induced by high glucose, apoptosis and necrosis were quantified. No differences were observed regarding the apoptotic index between low and high glucose cultures, but lactate dehydrogenase activity was increased in high glucose cultures. In conclusion, high glucose promotes necrotic cell death through H 2 O 2 formation, which may participate in the development of diabetic vasculopathy. British Journal of Pharmacology (2001) 133 , 967–974; doi: 10.1038/sj.bjp.0704184" @default.
- W2009363604 created "2016-06-24" @default.
- W2009363604 creator A5004792362 @default.
- W2009363604 creator A5013294389 @default.
- W2009363604 creator A5032060789 @default.
- W2009363604 creator A5035639454 @default.
- W2009363604 creator A5039961177 @default.
- W2009363604 creator A5051448235 @default.
- W2009363604 creator A5061334930 @default.
- W2009363604 creator A5070563602 @default.
- W2009363604 date "2001-08-01" @default.
- W2009363604 modified "2023-10-18" @default.
- W2009363604 title "High glucose induces cell death of cultured human aortic smooth muscle cells through the formation of hydrogen peroxide" @default.
- W2009363604 cites W1965283399 @default.
- W2009363604 cites W1970304246 @default.
- W2009363604 cites W1970677658 @default.
- W2009363604 cites W1982371349 @default.
- W2009363604 cites W1988176697 @default.
- W2009363604 cites W1996839970 @default.
- W2009363604 cites W1996963485 @default.
- W2009363604 cites W1999470250 @default.
- W2009363604 cites W2001870404 @default.
- W2009363604 cites W2006728188 @default.
- W2009363604 cites W2007880055 @default.
- W2009363604 cites W2013529909 @default.
- W2009363604 cites W2022646557 @default.
- W2009363604 cites W2023739408 @default.
- W2009363604 cites W2028169530 @default.
- W2009363604 cites W2028640382 @default.
- W2009363604 cites W2072560041 @default.
- W2009363604 cites W2072902969 @default.
- W2009363604 cites W2085225081 @default.
- W2009363604 cites W2090017683 @default.
- W2009363604 cites W2092145650 @default.
- W2009363604 cites W2094242175 @default.
- W2009363604 cites W2110342945 @default.
- W2009363604 cites W2115337518 @default.
- W2009363604 cites W2115484020 @default.
- W2009363604 cites W2123946565 @default.
- W2009363604 cites W2135097963 @default.
- W2009363604 cites W2137199906 @default.
- W2009363604 cites W2137302404 @default.
- W2009363604 cites W2140790576 @default.
- W2009363604 cites W2158968180 @default.
- W2009363604 cites W2340948203 @default.
- W2009363604 cites W2431495653 @default.
- W2009363604 cites W2769264260 @default.
- W2009363604 cites W4241954454 @default.
- W2009363604 cites W4251686161 @default.
- W2009363604 doi "https://doi.org/10.1038/sj.bjp.0704184" @default.
- W2009363604 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1572888" @default.
- W2009363604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11487505" @default.
- W2009363604 hasPublicationYear "2001" @default.
- W2009363604 type Work @default.
- W2009363604 sameAs 2009363604 @default.
- W2009363604 citedByCount "83" @default.
- W2009363604 countsByYear W20093636042012 @default.
- W2009363604 countsByYear W20093636042013 @default.
- W2009363604 countsByYear W20093636042014 @default.
- W2009363604 countsByYear W20093636042015 @default.
- W2009363604 countsByYear W20093636042016 @default.
- W2009363604 countsByYear W20093636042017 @default.
- W2009363604 countsByYear W20093636042018 @default.
- W2009363604 countsByYear W20093636042019 @default.
- W2009363604 countsByYear W20093636042020 @default.
- W2009363604 countsByYear W20093636042021 @default.
- W2009363604 crossrefType "journal-article" @default.
- W2009363604 hasAuthorship W2009363604A5004792362 @default.
- W2009363604 hasAuthorship W2009363604A5013294389 @default.
- W2009363604 hasAuthorship W2009363604A5032060789 @default.
- W2009363604 hasAuthorship W2009363604A5035639454 @default.
- W2009363604 hasAuthorship W2009363604A5039961177 @default.
- W2009363604 hasAuthorship W2009363604A5051448235 @default.
- W2009363604 hasAuthorship W2009363604A5061334930 @default.
- W2009363604 hasAuthorship W2009363604A5070563602 @default.
- W2009363604 hasBestOaLocation W20093636042 @default.
- W2009363604 hasConcept C126322002 @default.
- W2009363604 hasConcept C134018914 @default.
- W2009363604 hasConcept C1491633281 @default.
- W2009363604 hasConcept C181199279 @default.
- W2009363604 hasConcept C185592680 @default.
- W2009363604 hasConcept C190283241 @default.
- W2009363604 hasConcept C2776151105 @default.
- W2009363604 hasConcept C2777318727 @default.
- W2009363604 hasConcept C2778979269 @default.
- W2009363604 hasConcept C2779964046 @default.
- W2009363604 hasConcept C31573885 @default.
- W2009363604 hasConcept C503630168 @default.
- W2009363604 hasConcept C55493867 @default.
- W2009363604 hasConcept C62112901 @default.
- W2009363604 hasConcept C71924100 @default.
- W2009363604 hasConcept C79879829 @default.
- W2009363604 hasConcept C86803240 @default.
- W2009363604 hasConceptScore W2009363604C126322002 @default.
- W2009363604 hasConceptScore W2009363604C134018914 @default.
- W2009363604 hasConceptScore W2009363604C1491633281 @default.
- W2009363604 hasConceptScore W2009363604C181199279 @default.
- W2009363604 hasConceptScore W2009363604C185592680 @default.