FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009392199> ?p ?o ?g. }

• W2009392199 endingPage "6920" @default.
• W2009392199 startingPage "6914" @default.
• W2009392199 abstract "Using RT-PCR, a cDNA fragment of NADPH-cytochrome P450 oxidoreductase from silkworm, Bombyx mori, was cloned from three-day-old nondiapause eggs. RACE was used to isolate the ends of the DNA. The full-length cDNA obtained was composed of 3471 bp with an open reading frame encoding a protein of 687 amino-acid residues with a relative molecular mass of 77 700. The protein, fused with glutathione S-transferase, was expressed in Escherichia coli and purified to homogeneity. The fused protein not only had NADPH-dependent cytochrome c-reducing activity, but also acted as an electron carrier from NADPH to bovine adrenal 21-hydroxylase P450 in the steroid hydroxylation reaction, confirming that the protein is the silkworm NADPH-cytochrome P450 oxidoreductase. Ecdysone 20-hydroxylase activity in the nondiapause egg microsomes increased until the fourth day after oviposition, and then decreased, little being detected on the ninth day. An antibody raised against the P450 reductase inhibited the ecdysone hydroxylation. Immunoblot analyses of the microsomes indicated that the P450 reductase protein appeared distinctly in the three-day-old nondiapause eggs and, in contrast to the developmental pattern of ecdysone hydroxylase activity, continued to increase as the embryos developed. These results suggest that ecdysone hydroxylation in the early stage of embryogenesis is dependent on the presence of both P450 reductase and ecdysone 20-hydroxylase P450, but its gradual reduction in the later stage may be due to the decrease in the level of ecdysone 20-hydroxylase P450." @default.
• W2009392199 created "2016-06-24" @default.
• W2009392199 creator A5031128573 @default.
• W2009392199 creator A5035657531 @default.
• W2009392199 creator A5043012598 @default.
• W2009392199 creator A5044706467 @default.
• W2009392199 creator A5082185893 @default.
• W2009392199 date "2000-12-01" @default.
• W2009392199 modified "2023-10-12" @default.
• W2009392199 title "Molecular cloning of NADPH-cytochrome P450 oxidoreductase from silkworm eggs" @default.
• W2009392199 cites W1486315165 @default.
• W2009392199 cites W1519480480 @default.
• W2009392199 cites W1579297624 @default.
• W2009392199 cites W1589756962 @default.
• W2009392199 cites W1608322029 @default.
• W2009392199 cites W1971766079 @default.
• W2009392199 cites W1985504443 @default.
• W2009392199 cites W1986080936 @default.
• W2009392199 cites W1999411068 @default.
• W2009392199 cites W2004660568 @default.
• W2009392199 cites W2008364012 @default.
• W2009392199 cites W2014003227 @default.
• W2009392199 cites W2042487529 @default.
• W2009392199 cites W2048537666 @default.
• W2009392199 cites W2068241506 @default.
• W2009392199 cites W2072291087 @default.
• W2009392199 cites W2080682913 @default.
• W2009392199 cites W2113113971 @default.
• W2009392199 cites W2125551210 @default.
• W2009392199 cites W2913207692 @default.
• W2009392199 cites W4253486268 @default.
• W2009392199 cites W976594128 @default.
• W2009392199 doi "https://doi.org/10.1046/j.1432-1033.2000.01796.x" @default.
• W2009392199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11082204" @default.
• W2009392199 hasPublicationYear "2000" @default.
• W2009392199 type Work @default.
• W2009392199 sameAs 2009392199 @default.
• W2009392199 citedByCount "42" @default.
• W2009392199 countsByYear W20093921992012 @default.
• W2009392199 countsByYear W20093921992013 @default.
• W2009392199 countsByYear W20093921992014 @default.
• W2009392199 countsByYear W20093921992015 @default.
• W2009392199 countsByYear W20093921992016 @default.
• W2009392199 countsByYear W20093921992017 @default.
• W2009392199 countsByYear W20093921992018 @default.
• W2009392199 countsByYear W20093921992019 @default.
• W2009392199 countsByYear W20093921992020 @default.
• W2009392199 countsByYear W20093921992021 @default.
• W2009392199 countsByYear W20093921992022 @default.
• W2009392199 countsByYear W20093921992023 @default.
• W2009392199 crossrefType "journal-article" @default.
• W2009392199 hasAuthorship W2009392199A5031128573 @default.
• W2009392199 hasAuthorship W2009392199A5035657531 @default.
• W2009392199 hasAuthorship W2009392199A5043012598 @default.
• W2009392199 hasAuthorship W2009392199A5044706467 @default.
• W2009392199 hasAuthorship W2009392199A5082185893 @default.
• W2009392199 hasBestOaLocation W20093921991 @default.
• W2009392199 hasConcept C104317684 @default.
• W2009392199 hasConcept C109130350 @default.
• W2009392199 hasConcept C134651460 @default.
• W2009392199 hasConcept C153911025 @default.
• W2009392199 hasConcept C181199279 @default.
• W2009392199 hasConcept C187882448 @default.
• W2009392199 hasConcept C2777180181 @default.
• W2009392199 hasConcept C2778487026 @default.
• W2009392199 hasConcept C2779268744 @default.
• W2009392199 hasConcept C2779384962 @default.
• W2009392199 hasConcept C2780768313 @default.
• W2009392199 hasConcept C2781109383 @default.
• W2009392199 hasConcept C526171541 @default.
• W2009392199 hasConcept C55493867 @default.
• W2009392199 hasConcept C63932345 @default.
• W2009392199 hasConcept C86339819 @default.
• W2009392199 hasConcept C86803240 @default.
• W2009392199 hasConceptScore W2009392199C104317684 @default.
• W2009392199 hasConceptScore W2009392199C109130350 @default.
• W2009392199 hasConceptScore W2009392199C134651460 @default.
• W2009392199 hasConceptScore W2009392199C153911025 @default.
• W2009392199 hasConceptScore W2009392199C181199279 @default.
• W2009392199 hasConceptScore W2009392199C187882448 @default.
• W2009392199 hasConceptScore W2009392199C2777180181 @default.
• W2009392199 hasConceptScore W2009392199C2778487026 @default.
• W2009392199 hasConceptScore W2009392199C2779268744 @default.
• W2009392199 hasConceptScore W2009392199C2779384962 @default.
• W2009392199 hasConceptScore W2009392199C2780768313 @default.
• W2009392199 hasConceptScore W2009392199C2781109383 @default.
• W2009392199 hasConceptScore W2009392199C526171541 @default.
• W2009392199 hasConceptScore W2009392199C55493867 @default.
• W2009392199 hasConceptScore W2009392199C63932345 @default.
• W2009392199 hasConceptScore W2009392199C86339819 @default.
• W2009392199 hasConceptScore W2009392199C86803240 @default.
• W2009392199 hasIssue "23" @default.
• W2009392199 hasLocation W20093921991 @default.
• W2009392199 hasLocation W20093921992 @default.
• W2009392199 hasOpenAccess W2009392199 @default.
• W2009392199 hasPrimaryLocation W20093921991 @default.
• W2009392199 hasRelatedWork W1616452138 @default.
• W2009392199 hasRelatedWork W2017687363 @default.

• next