FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009409119> ?p ?o ?g. }

• W2009409119 endingPage "2049" @default.
• W2009409119 startingPage "2044" @default.
• W2009409119 abstract "Rapid growth in the biotechnology industry has led to a dramatic increase in attention to the protein folding problem. Understanding protein-folding pathways is essential to the production of biopharmaceuticals since commercial production of recombinant proteins often requires a protein-refolding process for recovery of high yields. Protein folding coupled to the formation of disulfide bonds presents one of the simplest approaches to studying folding intermediates. On-line capillary isoelectric focusing-electrospray ionization mass spectrometry (CIEF-ESIMS) is demonstrated for kinetic studies of disulfide bond-induced protein refolding. Refolding intermediates of bovine pancreatic ribonuclease A, a model system for this study, are blocked at different stages by alkylating free thiols with iodoacetate. The alkylation reaction results in the introduction of charge (-1) and mass (59) differences for each alkylation site, providing the means for predictable separation and direct identification of refolding intermediates using CIEF-ESIMS. Besides the observation of refolding intermediates containing different numbers of disulfide bonds and even mixed disulfides, the two-dimensional resolving power of CIEF-ESIMS allows the determination of conformational heterogeneity among groups of refolding intermediates." @default.
• W2009409119 created "2016-06-24" @default.
• W2009409119 creator A5006969898 @default.
• W2009409119 creator A5021497635 @default.
• W2009409119 creator A5090996937 @default.
• W2009409119 date "1998-04-10" @default.
• W2009409119 modified "2023-09-24" @default.
• W2009409119 title "Monitoring Protein Refolding Induced by Disulfide Formation Using Capillary Isoelectric Focusing-Electrospray Ionization Mass Spectrometry" @default.
• W2009409119 cites W1855896685 @default.
• W2009409119 cites W1966823236 @default.
• W2009409119 cites W1967543592 @default.
• W2009409119 cites W1980310871 @default.
• W2009409119 cites W1983247836 @default.
• W2009409119 cites W1988397041 @default.
• W2009409119 cites W1991498388 @default.
• W2009409119 cites W1999150153 @default.
• W2009409119 cites W2009543203 @default.
• W2009409119 cites W2033210824 @default.
• W2009409119 cites W2034432622 @default.
• W2009409119 cites W2040209976 @default.
• W2009409119 cites W2041484038 @default.
• W2009409119 cites W2062551574 @default.
• W2009409119 cites W2066623163 @default.
• W2009409119 cites W2080992873 @default.
• W2009409119 cites W2085419653 @default.
• W2009409119 cites W2085924900 @default.
• W2009409119 cites W2086446662 @default.
• W2009409119 cites W2153138530 @default.
• W2009409119 cites W2157006014 @default.
• W2009409119 cites W2160949470 @default.
• W2009409119 doi "https://doi.org/10.1021/ac9712963" @default.
• W2009409119 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9608843" @default.
• W2009409119 hasPublicationYear "1998" @default.
• W2009409119 type Work @default.
• W2009409119 sameAs 2009409119 @default.
• W2009409119 citedByCount "25" @default.
• W2009409119 countsByYear W20094091192012 @default.
• W2009409119 countsByYear W20094091192013 @default.
• W2009409119 countsByYear W20094091192014 @default.
• W2009409119 countsByYear W20094091192015 @default.
• W2009409119 countsByYear W20094091192016 @default.
• W2009409119 countsByYear W20094091192017 @default.
• W2009409119 crossrefType "journal-article" @default.
• W2009409119 hasAuthorship W2009409119A5006969898 @default.
• W2009409119 hasAuthorship W2009409119A5021497635 @default.
• W2009409119 hasAuthorship W2009409119A5090996937 @default.
• W2009409119 hasConcept C104317684 @default.
• W2009409119 hasConcept C119599485 @default.
• W2009409119 hasConcept C127413603 @default.
• W2009409119 hasConcept C161790260 @default.
• W2009409119 hasConcept C162356407 @default.
• W2009409119 hasConcept C164361826 @default.
• W2009409119 hasConcept C178790620 @default.
• W2009409119 hasConcept C181199279 @default.
• W2009409119 hasConcept C185592680 @default.
• W2009409119 hasConcept C204328495 @default.
• W2009409119 hasConcept C206759300 @default.
• W2009409119 hasConcept C21951064 @default.
• W2009409119 hasConcept C2776545253 @default.
• W2009409119 hasConcept C2776813977 @default.
• W2009409119 hasConcept C2778401398 @default.
• W2009409119 hasConcept C2778976237 @default.
• W2009409119 hasConcept C2780340462 @default.
• W2009409119 hasConcept C40684141 @default.
• W2009409119 hasConcept C43617362 @default.
• W2009409119 hasConcept C50476208 @default.
• W2009409119 hasConcept C55493867 @default.
• W2009409119 hasConcept C67705224 @default.
• W2009409119 hasConcept C96294017 @default.
• W2009409119 hasConceptScore W2009409119C104317684 @default.
• W2009409119 hasConceptScore W2009409119C119599485 @default.
• W2009409119 hasConceptScore W2009409119C127413603 @default.
• W2009409119 hasConceptScore W2009409119C161790260 @default.
• W2009409119 hasConceptScore W2009409119C162356407 @default.
• W2009409119 hasConceptScore W2009409119C164361826 @default.
• W2009409119 hasConceptScore W2009409119C178790620 @default.
• W2009409119 hasConceptScore W2009409119C181199279 @default.
• W2009409119 hasConceptScore W2009409119C185592680 @default.
• W2009409119 hasConceptScore W2009409119C204328495 @default.
• W2009409119 hasConceptScore W2009409119C206759300 @default.
• W2009409119 hasConceptScore W2009409119C21951064 @default.
• W2009409119 hasConceptScore W2009409119C2776545253 @default.
• W2009409119 hasConceptScore W2009409119C2776813977 @default.
• W2009409119 hasConceptScore W2009409119C2778401398 @default.
• W2009409119 hasConceptScore W2009409119C2778976237 @default.
• W2009409119 hasConceptScore W2009409119C2780340462 @default.
• W2009409119 hasConceptScore W2009409119C40684141 @default.
• W2009409119 hasConceptScore W2009409119C43617362 @default.
• W2009409119 hasConceptScore W2009409119C50476208 @default.
• W2009409119 hasConceptScore W2009409119C55493867 @default.
• W2009409119 hasConceptScore W2009409119C67705224 @default.
• W2009409119 hasConceptScore W2009409119C96294017 @default.
• W2009409119 hasIssue "10" @default.
• W2009409119 hasLocation W20094091191 @default.
• W2009409119 hasLocation W20094091192 @default.
• W2009409119 hasOpenAccess W2009409119 @default.
• W2009409119 hasPrimaryLocation W20094091191 @default.
• W2009409119 hasRelatedWork W121217726 @default.

• next