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- W2009421000 abstract "High specific activity [125I]triiodothyronine and [125I] thyroxine have been produced regularly by the chloramine T radioiodination method. Simultaneous production of [125I]triiodothyronine and [125I]thyroxine is usual when diiodothyronine or triiodothyronine are employed as the starting materials. Specific activities reached vary with the starting compound (diiodothyronine, triiodothyronine, thyroxine) used, as both substitution and, less readily, exchange of iodine atoms take place. Starting with diiodothyronine specific activities of approximately 2400 and 5200 Ci/g were achieved for [125I] triidothyronine and [125I] thyroxine, respectively, and, similarly, specific activities of approximately 1200 and 4000 Ci/g for [125I] triiodothyronine and [125I]-thyroxine, respectively, were reached when triiodothyronine was the starting material. [125I]Triiodoacetic acid and [125I]tetraiodoacetic acid have been produced in the same way from triiodoacetic acid. By column chromatography on Sephadex G-25 (fine), eluting with alkaline phosphate buffer, good separation of the radioiodinated products has been readily achieved.Studies on the stability of the radioiodinated hormones showed that 50% methanol, ethanol, propanol and propylene glycol were all equivalent as preserving agents and, further, that the stability of the radioiodinated hormones was linearly related to the concentration of these preserving agents." @default.
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- W2009421000 date "1967-01-01" @default.
- W2009421000 modified "2023-09-25" @default.
- W2009421000 title "Adsorption properties of iodotyrosines and derivatives on sephadex" @default.
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- W2009421000 doi "https://doi.org/10.1016/s0021-9673(01)85924-0" @default.
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