Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009432854> ?p ?o ?g. }
- W2009432854 endingPage "1752" @default.
- W2009432854 startingPage "1752" @default.
- W2009432854 abstract "In this paper, a new microfluidic array device has been fabricated with screen printing technology. In contrast to traditional microfabrication processes, our method is simple, inexpensive and also suitable for mass production. The device is used for sandwich-type electrochemical immunoassay, in which probes are covalently attached to the electrode surface via electropolymerized polypyrrole propylic acid (PPA) film. This novel microfluidic system enables the whole array preparation and detection processes, including the probe immobilization, sample injection, enzyme incubation and electrochemical detection, to be conducted in the sealed microchannels. For a demonstration, mouse IgG is selected as the target analyte and its detection is realized by sandwich ELISA with goat anti-mouse IgG, rat anti-mouse IgG (conjugated to alkaline phosphatase) and p-aminophenyl phosphate (PAPP) as the primary antibody, second antibody, and enzyme substrate, respectively. A detection limit of 10 ng mL(-1) (67 pM) is achieved with a dynamic range of 100 ng mL(-1)-10 microg mL(-1). In addition, anti-goat IgG is also immobilized as an alternative probe to test mouse IgG in the solution, in order to demonstrate the multiplexing capability as well as the specificity of the device. As expected, the electrochemical responses are much lower than that using anti-mouse IgG as the probe, indicating good selectivity of the immunoassay device. These results indicate a great promise toward the development of miniaturized, low-cost protein biochips for clinical, forensics, environmental, and pharmaceutical applications." @default.
- W2009432854 created "2016-06-24" @default.
- W2009432854 creator A5003330072 @default.
- W2009432854 creator A5018734784 @default.
- W2009432854 creator A5036658104 @default.
- W2009432854 creator A5046867711 @default.
- W2009432854 creator A5086154174 @default.
- W2009432854 date "2007-01-01" @default.
- W2009432854 modified "2023-10-08" @default.
- W2009432854 title "Screen-printed microfluidic device for electrochemical immunoassay" @default.
- W2009432854 cites W1562528808 @default.
- W2009432854 cites W1972466773 @default.
- W2009432854 cites W1979339863 @default.
- W2009432854 cites W1985537766 @default.
- W2009432854 cites W1988654953 @default.
- W2009432854 cites W1990788276 @default.
- W2009432854 cites W1993707958 @default.
- W2009432854 cites W2013992558 @default.
- W2009432854 cites W2014322909 @default.
- W2009432854 cites W2016478959 @default.
- W2009432854 cites W2018246262 @default.
- W2009432854 cites W2020664976 @default.
- W2009432854 cites W2021108035 @default.
- W2009432854 cites W2024237260 @default.
- W2009432854 cites W2031610738 @default.
- W2009432854 cites W2035672458 @default.
- W2009432854 cites W2044372170 @default.
- W2009432854 cites W2052489824 @default.
- W2009432854 cites W2066202272 @default.
- W2009432854 cites W2069570927 @default.
- W2009432854 cites W2069633260 @default.
- W2009432854 cites W2078782272 @default.
- W2009432854 cites W2088831546 @default.
- W2009432854 cites W2091117170 @default.
- W2009432854 cites W2094092228 @default.
- W2009432854 cites W2095054112 @default.
- W2009432854 cites W2100804670 @default.
- W2009432854 cites W2155213995 @default.
- W2009432854 doi "https://doi.org/10.1039/b712394a" @default.
- W2009432854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18030397" @default.
- W2009432854 hasPublicationYear "2007" @default.
- W2009432854 type Work @default.
- W2009432854 sameAs 2009432854 @default.
- W2009432854 citedByCount "104" @default.
- W2009432854 countsByYear W20094328542012 @default.
- W2009432854 countsByYear W20094328542013 @default.
- W2009432854 countsByYear W20094328542014 @default.
- W2009432854 countsByYear W20094328542015 @default.
- W2009432854 countsByYear W20094328542016 @default.
- W2009432854 countsByYear W20094328542017 @default.
- W2009432854 countsByYear W20094328542018 @default.
- W2009432854 countsByYear W20094328542019 @default.
- W2009432854 countsByYear W20094328542020 @default.
- W2009432854 countsByYear W20094328542021 @default.
- W2009432854 countsByYear W20094328542022 @default.
- W2009432854 countsByYear W20094328542023 @default.
- W2009432854 crossrefType "journal-article" @default.
- W2009432854 hasAuthorship W2009432854A5003330072 @default.
- W2009432854 hasAuthorship W2009432854A5018734784 @default.
- W2009432854 hasAuthorship W2009432854A5036658104 @default.
- W2009432854 hasAuthorship W2009432854A5046867711 @default.
- W2009432854 hasAuthorship W2009432854A5086154174 @default.
- W2009432854 hasConcept C10390740 @default.
- W2009432854 hasConcept C111368507 @default.
- W2009432854 hasConcept C119128265 @default.
- W2009432854 hasConcept C127313418 @default.
- W2009432854 hasConcept C136525101 @default.
- W2009432854 hasConcept C142724271 @default.
- W2009432854 hasConcept C147789679 @default.
- W2009432854 hasConcept C159654299 @default.
- W2009432854 hasConcept C171250308 @default.
- W2009432854 hasConcept C17525397 @default.
- W2009432854 hasConcept C178790620 @default.
- W2009432854 hasConcept C180577832 @default.
- W2009432854 hasConcept C185592680 @default.
- W2009432854 hasConcept C192562407 @default.
- W2009432854 hasConcept C203014093 @default.
- W2009432854 hasConcept C204787440 @default.
- W2009432854 hasConcept C21269302 @default.
- W2009432854 hasConcept C2777232022 @default.
- W2009432854 hasConcept C2777289219 @default.
- W2009432854 hasConcept C2778810118 @default.
- W2009432854 hasConcept C2780420688 @default.
- W2009432854 hasConcept C43617362 @default.
- W2009432854 hasConcept C527607 @default.
- W2009432854 hasConcept C52859227 @default.
- W2009432854 hasConcept C71924100 @default.
- W2009432854 hasConcept C8673954 @default.
- W2009432854 hasConcept C86803240 @default.
- W2009432854 hasConcept C87892846 @default.
- W2009432854 hasConceptScore W2009432854C10390740 @default.
- W2009432854 hasConceptScore W2009432854C111368507 @default.
- W2009432854 hasConceptScore W2009432854C119128265 @default.
- W2009432854 hasConceptScore W2009432854C127313418 @default.
- W2009432854 hasConceptScore W2009432854C136525101 @default.
- W2009432854 hasConceptScore W2009432854C142724271 @default.
- W2009432854 hasConceptScore W2009432854C147789679 @default.
- W2009432854 hasConceptScore W2009432854C159654299 @default.