FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009489468> ?p ?o ?g. }

• W2009489468 endingPage "54" @default.
• W2009489468 startingPage "47" @default.
• W2009489468 abstract "Microbial communities that thrive in subterranean consolidated sediments are largely unknown owing to the difficulty of extracting DNA. As this difficulty is often attributed to DNA binding onto the silica-bearing sediment matrix, we developed a DNA extraction method for consolidated sediment from the deep subsurface in which silica minerals were dissolved by being heated under alkaline conditions. NaOH concentrations (0.07 and 0.33 N), incubation temperatures (65 and 94 °C) and incubation times (30–90 min) before neutralization were evaluated based on the copy number of extracted prokaryotic DNA. Prokaryotic DNA was detected by quantitative PCR analysis after heating the sediment sample at 94 °C in 0.33 N NaOH solution for 50–80 min. Results of 16S rRNA gene sequence analysis of the extracted DNA were all consistent with regard to the dominant occurrence of the metallophilic bacterium, Cupriavidus metallidurans, and Pseudomonas spp. Mineralogical analysis revealed that the dissolution of a silica mineral (opal-CT) during alkaline treatment was maximized at 94 °C in 0.33 N NaOH solution for 50 min, which may have resulted in the release of DNA into solution. Because the optimized protocol for DNA extraction is applicable to subterranean consolidated sediments from a different locality, the method developed here has the potential to expand our understanding of the microbial community structure of the deep biosphere." @default.
• W2009489468 created "2016-06-24" @default.
• W2009489468 creator A5023857711 @default.
• W2009489468 creator A5031433497 @default.
• W2009489468 creator A5061777630 @default.
• W2009489468 creator A5065439474 @default.
• W2009489468 creator A5075352757 @default.
• W2009489468 creator A5075842667 @default.
• W2009489468 date "2011-11-23" @default.
• W2009489468 modified "2023-10-17" @default.
• W2009489468 title "A new DNA extraction method by controlled alkaline treatments from consolidated subsurface sediments" @default.
• W2009489468 cites W1498206429 @default.
• W2009489468 cites W1514657191 @default.
• W2009489468 cites W1536577349 @default.
• W2009489468 cites W1588533534 @default.
• W2009489468 cites W1638517249 @default.
• W2009489468 cites W1881308330 @default.
• W2009489468 cites W1968951618 @default.
• W2009489468 cites W1980453934 @default.
• W2009489468 cites W1980761392 @default.
• W2009489468 cites W1983231276 @default.
• W2009489468 cites W1984637730 @default.
• W2009489468 cites W1993505867 @default.
• W2009489468 cites W1994194533 @default.
• W2009489468 cites W2021758123 @default.
• W2009489468 cites W2043959425 @default.
• W2009489468 cites W2044501726 @default.
• W2009489468 cites W2062994865 @default.
• W2009489468 cites W2079291885 @default.
• W2009489468 cites W2084072969 @default.
• W2009489468 cites W2087482496 @default.
• W2009489468 cites W2118283136 @default.
• W2009489468 cites W2124012443 @default.
• W2009489468 cites W2130449659 @default.
• W2009489468 cites W2153823136 @default.
• W2009489468 cites W2165115518 @default.
• W2009489468 cites W2171879944 @default.
• W2009489468 cites W4250297812 @default.
• W2009489468 doi "https://doi.org/10.1111/j.1574-6968.2011.02437.x" @default.
• W2009489468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22092362" @default.
• W2009489468 hasPublicationYear "2011" @default.
• W2009489468 type Work @default.
• W2009489468 sameAs 2009489468 @default.
• W2009489468 citedByCount "26" @default.
• W2009489468 countsByYear W20094894682013 @default.
• W2009489468 countsByYear W20094894682014 @default.
• W2009489468 countsByYear W20094894682015 @default.
• W2009489468 countsByYear W20094894682016 @default.
• W2009489468 countsByYear W20094894682017 @default.
• W2009489468 countsByYear W20094894682018 @default.
• W2009489468 countsByYear W20094894682019 @default.
• W2009489468 countsByYear W20094894682020 @default.
• W2009489468 countsByYear W20094894682021 @default.
• W2009489468 countsByYear W20094894682022 @default.
• W2009489468 countsByYear W20094894682023 @default.
• W2009489468 crossrefType "journal-article" @default.
• W2009489468 hasAuthorship W2009489468A5023857711 @default.
• W2009489468 hasAuthorship W2009489468A5031433497 @default.
• W2009489468 hasAuthorship W2009489468A5061777630 @default.
• W2009489468 hasAuthorship W2009489468A5065439474 @default.
• W2009489468 hasAuthorship W2009489468A5075352757 @default.
• W2009489468 hasAuthorship W2009489468A5075842667 @default.
• W2009489468 hasBestOaLocation W20094894681 @default.
• W2009489468 hasConcept C104317684 @default.
• W2009489468 hasConcept C107872376 @default.
• W2009489468 hasConcept C151730666 @default.
• W2009489468 hasConcept C178790620 @default.
• W2009489468 hasConcept C183586093 @default.
• W2009489468 hasConcept C185592680 @default.
• W2009489468 hasConcept C199289684 @default.
• W2009489468 hasConcept C25642318 @default.
• W2009489468 hasConcept C2816523 @default.
• W2009489468 hasConcept C43617362 @default.
• W2009489468 hasConcept C4725764 @default.
• W2009489468 hasConcept C49105822 @default.
• W2009489468 hasConcept C523546767 @default.
• W2009489468 hasConcept C54355233 @default.
• W2009489468 hasConcept C552990157 @default.
• W2009489468 hasConcept C55493867 @default.
• W2009489468 hasConcept C78063203 @default.
• W2009489468 hasConcept C81407943 @default.
• W2009489468 hasConcept C86803240 @default.
• W2009489468 hasConcept C88380143 @default.
• W2009489468 hasConceptScore W2009489468C104317684 @default.
• W2009489468 hasConceptScore W2009489468C107872376 @default.
• W2009489468 hasConceptScore W2009489468C151730666 @default.
• W2009489468 hasConceptScore W2009489468C178790620 @default.
• W2009489468 hasConceptScore W2009489468C183586093 @default.
• W2009489468 hasConceptScore W2009489468C185592680 @default.
• W2009489468 hasConceptScore W2009489468C199289684 @default.
• W2009489468 hasConceptScore W2009489468C25642318 @default.
• W2009489468 hasConceptScore W2009489468C2816523 @default.
• W2009489468 hasConceptScore W2009489468C43617362 @default.
• W2009489468 hasConceptScore W2009489468C4725764 @default.
• W2009489468 hasConceptScore W2009489468C49105822 @default.
• W2009489468 hasConceptScore W2009489468C523546767 @default.
• W2009489468 hasConceptScore W2009489468C54355233 @default.
• W2009489468 hasConceptScore W2009489468C552990157 @default.

• next