FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009498922> ?p ?o ?g. }

• W2009498922 endingPage "2614" @default.
• W2009498922 startingPage "2614" @default.
• W2009498922 abstract "Spermatogonial stem cells (SSCs) are a unique type of stem cells in that they transmit genetic information to the next generation by producing sperms. Studies of SSC proliferation and differentiation have been hampered by the inability of reconstructing these processes in vitro, particularly in a serum-free culture system. Several groups have reported the long term culture of SSCs during which SSCs self-renew and restore spermatogenesis when transplanted back to recipient testes. However, different protocols and mice with particular genetic background have been used by different laboratories, and the techniques have not been adopted widely. In the present study, we first established a SSC isolation and culture system composed of differential adherence selection of SSCs, serum-free medium and mouse embryonic fibroblast (MEF) feeder cells. SSCs from KM pups could be cultured on MEF feeders in StemPro-34 SFM Medium supplemented with glial cell line-derived neurotrophic factor (GDNF), soluble GDNF family receptor alpha-1 (GFRa1) and basic fibroblast growth factor (bFGF) for 1 month. These SSCs were characterized morphologically and by examining the expression of marker genes. Expression of Oct4 and Sox2, which are crucial factors in embryonic stem cell (ESC) self-renewal, were detected in our cultured SSCs, suggesting that SSCs may share with ESCs some common mechanisms in self-renewal regulation. We also found that LIF had no effect on the proliferation of cultured SSCs derived from KM mice." @default.
• W2009498922 created "2016-06-24" @default.
• W2009498922 creator A5002313093 @default.
• W2009498922 creator A5025966542 @default.
• W2009498922 creator A5034934121 @default.
• W2009498922 creator A5082678251 @default.
• W2009498922 creator A5083370352 @default.
• W2009498922 creator A5088339977 @default.
• W2009498922 date "2006-01-01" @default.
• W2009498922 modified "2023-09-25" @default.
• W2009498922 title "In vitro propagation of spermatogonial stem cells from KM mice" @default.
• W2009498922 cites W1423307790 @default.
• W2009498922 cites W1516316082 @default.
• W2009498922 cites W1970706325 @default.
• W2009498922 cites W1976021779 @default.
• W2009498922 cites W1979981202 @default.
• W2009498922 cites W1981088384 @default.
• W2009498922 cites W1989381407 @default.
• W2009498922 cites W1992160731 @default.
• W2009498922 cites W1993491850 @default.
• W2009498922 cites W1995559307 @default.
• W2009498922 cites W1999865455 @default.
• W2009498922 cites W2002654571 @default.
• W2009498922 cites W2004211510 @default.
• W2009498922 cites W2009259931 @default.
• W2009498922 cites W2018855387 @default.
• W2009498922 cites W2021094253 @default.
• W2009498922 cites W2021971921 @default.
• W2009498922 cites W2031334250 @default.
• W2009498922 cites W2035251095 @default.
• W2009498922 cites W2036291312 @default.
• W2009498922 cites W2040285486 @default.
• W2009498922 cites W2040605613 @default.
• W2009498922 cites W2045677887 @default.
• W2009498922 cites W2045887697 @default.
• W2009498922 cites W2050518767 @default.
• W2009498922 cites W2052490309 @default.
• W2009498922 cites W2061330837 @default.
• W2009498922 cites W2065651113 @default.
• W2009498922 cites W2073738799 @default.
• W2009498922 cites W2085171607 @default.
• W2009498922 cites W2086293569 @default.
• W2009498922 cites W2088514277 @default.
• W2009498922 cites W2096345239 @default.
• W2009498922 cites W2102465334 @default.
• W2009498922 cites W2104457965 @default.
• W2009498922 cites W2105361655 @default.
• W2009498922 cites W2107575419 @default.
• W2009498922 cites W2108451330 @default.
• W2009498922 cites W2110804359 @default.
• W2009498922 cites W2118290853 @default.
• W2009498922 cites W2118487725 @default.
• W2009498922 cites W2123986423 @default.
• W2009498922 cites W2126851387 @default.
• W2009498922 cites W2128786355 @default.
• W2009498922 cites W2131238404 @default.
• W2009498922 cites W2138442302 @default.
• W2009498922 cites W2139789628 @default.
• W2009498922 cites W2141128578 @default.
• W2009498922 cites W2144134929 @default.
• W2009498922 cites W2148628757 @default.
• W2009498922 cites W2150163104 @default.
• W2009498922 cites W2165135585 @default.
• W2009498922 cites W2166481218 @default.
• W2009498922 cites W2167226908 @default.
• W2009498922 cites W2169529166 @default.
• W2009498922 cites W2170748214 @default.
• W2009498922 cites W233481338 @default.
• W2009498922 cites W30145574 @default.
• W2009498922 doi "https://doi.org/10.2741/1995" @default.
• W2009498922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16720338" @default.
• W2009498922 hasPublicationYear "2006" @default.
• W2009498922 type Work @default.
• W2009498922 sameAs 2009498922 @default.
• W2009498922 citedByCount "20" @default.
• W2009498922 countsByYear W20094989222012 @default.
• W2009498922 countsByYear W20094989222013 @default.
• W2009498922 countsByYear W20094989222014 @default.
• W2009498922 countsByYear W20094989222016 @default.
• W2009498922 countsByYear W20094989222017 @default.
• W2009498922 crossrefType "journal-article" @default.
• W2009498922 hasAuthorship W2009498922A5002313093 @default.
• W2009498922 hasAuthorship W2009498922A5025966542 @default.
• W2009498922 hasAuthorship W2009498922A5034934121 @default.
• W2009498922 hasAuthorship W2009498922A5082678251 @default.
• W2009498922 hasAuthorship W2009498922A5083370352 @default.
• W2009498922 hasAuthorship W2009498922A5088339977 @default.
• W2009498922 hasConcept C104317684 @default.
• W2009498922 hasConcept C145103041 @default.
• W2009498922 hasConcept C153911025 @default.
• W2009498922 hasConcept C160539049 @default.
• W2009498922 hasConcept C170493617 @default.
• W2009498922 hasConcept C185856081 @default.
• W2009498922 hasConcept C202751555 @default.
• W2009498922 hasConcept C203014093 @default.
• W2009498922 hasConcept C2775960820 @default.
• W2009498922 hasConcept C2777587049 @default.
• W2009498922 hasConcept C2777933648 @default.

• next