FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009502018> ?p ?o ?g. }

• W2009502018 endingPage "1570" @default.
• W2009502018 startingPage "1553" @default.
• W2009502018 abstract "Studies of intracellular hepatitis C virus (HCV) RNA-dependent RNA polymerase activity (RdRp activity) have been limited by the poor replicative capacity of HCV in cell culture. We have developed a method that allows for the measurement of HCV specific RdRp activity in eukaryotic cells. This method is based on the transient expression of the HCV polymerase and its templates under the control of the T7 promoter in the presence of an infection with recombinant vaccinia virus (vTF7-3) expressing the bacteriophage T7 DNA-dependent RNA polymerase. Both negative-strand and positive-strand RNA synthesis were characterised, and the role of the other HCV non-structural proteins for polymerase activity was assessed. With this assay we were able to show that: a) Intracellular HCV RdRp activity is not restricted to, but is higher for templates containing HCV specific sequences, b) The HCV polymerase is active within the polyprotein precursor, c) Cleavage of NS5b from the polyprotein precursor does not determine template specificity, and d) HCV RdRp activity is higher in the presence of the other HCV non-structural proteins and lower within a protease-deficient polyprotein precursor. This method allows the measurement of intracellular HCV polymerase activity and may be used to test substances against the HCV polymerase in search of potential drugs for anti-HCV therapy." @default.
• W2009502018 created "2016-06-24" @default.
• W2009502018 creator A5013369380 @default.
• W2009502018 creator A5051880640 @default.
• W2009502018 creator A5075343449 @default.
• W2009502018 date "2001-08-01" @default.
• W2009502018 modified "2023-10-18" @default.
• W2009502018 title "Intracellular hepatitis C virus RNA-dependent RNA polymerase activity" @default.
• W2009502018 cites W1556286201 @default.
• W2009502018 cites W1595507615 @default.
• W2009502018 cites W1735561674 @default.
• W2009502018 cites W1821941363 @default.
• W2009502018 cites W1928472258 @default.
• W2009502018 cites W1932632320 @default.
• W2009502018 cites W1965505230 @default.
• W2009502018 cites W1965962980 @default.
• W2009502018 cites W1973165481 @default.
• W2009502018 cites W1975705587 @default.
• W2009502018 cites W1976844675 @default.
• W2009502018 cites W1979919755 @default.
• W2009502018 cites W1982831730 @default.
• W2009502018 cites W1983149942 @default.
• W2009502018 cites W1988834597 @default.
• W2009502018 cites W1993154139 @default.
• W2009502018 cites W2000174554 @default.
• W2009502018 cites W2003887562 @default.
• W2009502018 cites W2005411001 @default.
• W2009502018 cites W2006076593 @default.
• W2009502018 cites W2006309458 @default.
• W2009502018 cites W2021108691 @default.
• W2009502018 cites W2021451720 @default.
• W2009502018 cites W2022878150 @default.
• W2009502018 cites W2027379295 @default.
• W2009502018 cites W2027613773 @default.
• W2009502018 cites W2045378360 @default.
• W2009502018 cites W2053100601 @default.
• W2009502018 cites W2053996087 @default.
• W2009502018 cites W2064428351 @default.
• W2009502018 cites W2076682837 @default.
• W2009502018 cites W2080181710 @default.
• W2009502018 cites W2080383544 @default.
• W2009502018 cites W2090326661 @default.
• W2009502018 cites W2098897538 @default.
• W2009502018 cites W2106870343 @default.
• W2009502018 cites W2116621112 @default.
• W2009502018 cites W2133401977 @default.
• W2009502018 cites W2133580842 @default.
• W2009502018 cites W2144634347 @default.
• W2009502018 cites W2146236851 @default.
• W2009502018 cites W2417213807 @default.
• W2009502018 doi "https://doi.org/10.1007/s007050170078" @default.
• W2009502018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11676417" @default.
• W2009502018 hasPublicationYear "2001" @default.
• W2009502018 type Work @default.
• W2009502018 sameAs 2009502018 @default.
• W2009502018 citedByCount "5" @default.
• W2009502018 countsByYear W20095020182016 @default.
• W2009502018 crossrefType "journal-article" @default.
• W2009502018 hasAuthorship W2009502018A5013369380 @default.
• W2009502018 hasAuthorship W2009502018A5051880640 @default.
• W2009502018 hasAuthorship W2009502018A5075343449 @default.
• W2009502018 hasBestOaLocation W20095020181 @default.
• W2009502018 hasConcept C104317684 @default.
• W2009502018 hasConcept C153911025 @default.
• W2009502018 hasConcept C159047783 @default.
• W2009502018 hasConcept C181199279 @default.
• W2009502018 hasConcept C194575299 @default.
• W2009502018 hasConcept C2522874641 @default.
• W2009502018 hasConcept C25281209 @default.
• W2009502018 hasConcept C2776408679 @default.
• W2009502018 hasConcept C2776441376 @default.
• W2009502018 hasConcept C2776449523 @default.
• W2009502018 hasConcept C2780048736 @default.
• W2009502018 hasConcept C2781426373 @default.
• W2009502018 hasConcept C2781463415 @default.
• W2009502018 hasConcept C41258723 @default.
• W2009502018 hasConcept C547475151 @default.
• W2009502018 hasConcept C55493867 @default.
• W2009502018 hasConcept C67705224 @default.
• W2009502018 hasConcept C82381507 @default.
• W2009502018 hasConcept C83100098 @default.
• W2009502018 hasConcept C86803240 @default.
• W2009502018 hasConceptScore W2009502018C104317684 @default.
• W2009502018 hasConceptScore W2009502018C153911025 @default.
• W2009502018 hasConceptScore W2009502018C159047783 @default.
• W2009502018 hasConceptScore W2009502018C181199279 @default.
• W2009502018 hasConceptScore W2009502018C194575299 @default.
• W2009502018 hasConceptScore W2009502018C2522874641 @default.
• W2009502018 hasConceptScore W2009502018C25281209 @default.
• W2009502018 hasConceptScore W2009502018C2776408679 @default.
• W2009502018 hasConceptScore W2009502018C2776441376 @default.
• W2009502018 hasConceptScore W2009502018C2776449523 @default.
• W2009502018 hasConceptScore W2009502018C2780048736 @default.
• W2009502018 hasConceptScore W2009502018C2781426373 @default.
• W2009502018 hasConceptScore W2009502018C2781463415 @default.
• W2009502018 hasConceptScore W2009502018C41258723 @default.
• W2009502018 hasConceptScore W2009502018C547475151 @default.
• W2009502018 hasConceptScore W2009502018C55493867 @default.

• next