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- W2009533013 abstract "In order to study a 19-kDa protein (p19) of Campylobacter jejuni, we purified this protein to homogeneity from C. jejuni strain 81176 by anion exchange chromatography. The molecular weight of the native protein is 19,000 daltons. P19 was found to be acidic with an isoelectric point of 4.8 and was located in the periplasmic space of the bacteria. The 20 N-terminal amino acids were sequenced and no significant similarities with known proteins were shown. A monoclonal antibody showed that p19 is conserved in the 2 species C. jejuni and C. coli. Analysis of sera from 23 patients with a Campylobacter-related infection indicated that p19 is not immunogenic during natural infection in man. The gene encoding p19 was cloned and no strong homologies with known sequences were identified. The preparation of a knockout mutant in p19 will enable the investigation of the function of this cell wall component of Campylobacter. Afin d'étudier une protéine de 19 kDa de Campylobacter jejuni, nous avons purifié cette protéine à partir de la souche 81176 par chromatographie d'échange d'ions. Le poids moléculaire de la protéine native est de 19.000 daltons. Cette protéine est acide, avec un point isoélectrique de 4.8, et elle est localisée dans l'espace périplasmique de la bactérie. Les 20 acides aminés N-terminaux ont été séquencés: aucune similarité significative avec des protéines connues n'a été mise en évidence. L'obtention d'un anticorps monoclonal a permis de montrer que la protéine p19 est conservée chez les 2 espèces, C. jejuni et C. coli. L'analyse des sérums de 23 patients ayant développé une infection àC. jejuni montre que p19 n'est pas immunogène lors de l'infection naturelle chez l'homme. Le gène codant pour la p19 a été cloné et n'a montré aucune homologie importante avec des séquences connues. La préparation d'un mutant délété en p19 est en cours et permettra d'étudier la fonction de ce composant pariétal de Campylobacter." @default.
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- W2009533013 date "1998-02-01" @default.
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- W2009533013 title "Characterization and gene sequencing of a 19-kDa periplasmic protein of Campylobacter jejuni/coli" @default.
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- W2009533013 doi "https://doi.org/10.1016/s0923-2508(98)80025-9" @default.
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