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- W2009543804 abstract "Riboswitches are cis-acting regulatory elements broadly distributed in bacterial mRNAs that control a wide range of critical metabolic activities. Expression is governed by two distinct domains within the mRNA leader: a sensory 'aptamer domain' and a regulatory 'expression platform'. Riboswitches have also received considerable attention as important tools in synthetic biology because of their conceptually simple structure and the ability to obtain aptamers that bind almost any conceivable small molecule using in vitro selection (referred to as SELEX). In the design of artificial riboswitches, a significant hurdle has been to couple the two domains enabling their efficient communication. We previously demonstrated that biological transcriptional 'OFF' expression platforms are easily coupled to diverse aptamers, both biological and SELEX-derived, using simple design rules. Here, we present two modular transcriptional 'ON' riboswitch expression platforms that are also capable of hosting foreign aptamers. We demonstrate that these biological parts can be used to facilely generate artificial chimeric riboswitches capable of robustly regulating transcription both in vitro and in vivo. We expect that these modular expression platforms will be of great utility for various synthetic biological applications that use RNA-based biosensors." @default.
- W2009543804 created "2016-06-24" @default.
- W2009543804 creator A5004642468 @default.
- W2009543804 creator A5017593523 @default.
- W2009543804 creator A5034743607 @default.
- W2009543804 date "2013-09-02" @default.
- W2009543804 modified "2023-10-17" @default.
- W2009543804 title "Engineering modular ‘ON’ RNA switches using biological components" @default.
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- W2009543804 doi "https://doi.org/10.1093/nar/gkt787" @default.
- W2009543804 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3905868" @default.
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