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• W2009551468 abstract "Summary Bull spermatozoa were examined for the presence and localization of constitutive Nitric Oxide Synthase (NOS), as nitric oxide (NO) is involved in calcium‐dependent capacitation. In bull spermatozoa, NO generation is enhanced by l ‐arginine (3 μ m ) and abolished by the NOS‐inhibitor N ‐nitro‐ l ‐arginine methyl ester ( l ‐NAME). In addition, presence of NOS in bull spermatozoa was verified by immunohistochemistry, revealing the existence of both neuronal NOS (nNOS) and endothelial NOS (eNOS) immunoreaction. These findings were confirmed by Western blot technique, showing immunoreactive bands at 161 kDa (nNOS) and 133 kDa (eNOS). Confocal laser microscopy localized nNOS related immunofluorescence at the acrosome cap of sperms and their flagellum‐mainpart. This technique also identified eNOS staining spread over the spermatozoan head. In conclusion, immunohistochemistry, Western blot technique, and NO generation suggest the presence of n‐ and eNOS in bull spermatozoa." @default.
• W2009551468 created "2016-06-24" @default.
• W2009551468 creator A5016665543 @default.
• W2009551468 creator A5052975327 @default.
• W2009551468 date "2003-12-01" @default.
• W2009551468 modified "2023-10-15" @default.
• W2009551468 title "Detection and Localization of Two Constitutive NOS Isoforms in Bull Spermatozoa" @default.
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• W2009551468 doi "https://doi.org/10.1111/j.1439-0264.2003.00459.x" @default.
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