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• W2009572926 abstract "We demonstrate how the orientation and ordering of DNA bases in ultrahigh vacuum (UHV) and ambient environments can be determined using complementary spectroscopic methods. Near-edge X-ray absorption fine structure (NEXAFS) with fluorescence detection, X-ray photoelectron (XPS), and Fourier transform infrared (FTIR) spectroscopies are used to quantify the coverage, chemical composition, orientation, and ordering of thymine bases in model self-assembled monolayers of thymine homo-oligonucleotides [oligo(dT)] on gold. We find that, in monolayers of thiol-modified oligo(dT), thymine bases tend to orient parallel to the Au substrate, and this preferential orientation is significantly more pronounced in monolayers of thiolated 5-mers compared to 25-mers. We interpret this preferential orientation as a signature of significant correlations (local ordering) between individual nuleobases, which offers a way to quantify and compare nucleobase interactions in films under both ambient and UHV conditions." @default.
• W2009572926 created "2016-06-24" @default.
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• W2009572926 date "2005-12-14" @default.
• W2009572926 modified "2023-10-18" @default.
• W2009572926 title "Nucleobase Orientation and Ordering in Films of Single-Stranded DNA on Gold" @default.
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• W2009572926 doi "https://doi.org/10.1021/ja052443e" @default.
• W2009572926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16390092" @default.
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