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• W2009597954 abstract "A procedure is described for the direct immobilization of thrombin on carbon paste and solid carbon electrodes with good reproducibility and improved stability for the enzyme. Typically, the radiolabelling of thrombin with iodine-125 indicated that enzyme densities of about 2 × 10−13 mol cm−2 could be achieved at saturation. This gave a rate of coverage of about 20% of the electrochemical area. The catalytic activities and the kinetic parameters were estimated under forced convection conditions with a specific substrate (S-2238) provided with chromogenic properties and dissolved in the solution. No major changes in Km or Vm were found to be adsorption. However, increased activities were detected when adsorption was conducted at positive prepolarization of the electrode or after potential cycling in the presence of p-nitroaniline. A pattern of interaction with the surface sites of adsorption is proposed." @default.
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• W2009597954 date "1992-02-01" @default.
• W2009597954 modified "2023-09-26" @default.
• W2009597954 title "Potential- and surface-dependent behaviour of thrombin adsorbed on carbon electrodes" @default.
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• W2009597954 doi "https://doi.org/10.1016/0003-2670(92)85178-9" @default.
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