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- W2009632907 abstract "We report herein the development of a DNA binding and reactivity assay methodology based on the in-frame protein expression scheme. The stringent requirement for the precise in vivo translation of sequence information into an amplified readout signal, coupled with a strand displacement-based sequence recognition strategy and antibiotic-conferred channel selection capability, has allowed for the elaboration of a DNA diagnostic protocol with high sensitivity, high selectivity, and multiple-target detection capacity. The efficacy of the proposed DNA detection scheme is further demonstrated through the assay on a genomic target. Importantly, the uniquely structured signal blocking/generation DNA architecture provides a generic handle for the examination of DNA reaction, DNA-small molecule binding, and DNA-protein binding events. The exceptional versatility associated with the one-platform system is anticipated to contribute to the screening and therefore understanding of a diversity of DNA-centered biological phenomena." @default.
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- W2009632907 date "2013-01-01" @default.
- W2009632907 modified "2023-10-16" @default.
- W2009632907 title "DNA binding and reactivity assays based on in-frame protein expression" @default.
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- W2009632907 doi "https://doi.org/10.1039/c2sc21464d" @default.
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