FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009653722> ?p ?o ?g. }

• W2009653722 endingPage "1307" @default.
• W2009653722 startingPage "1299" @default.
• W2009653722 abstract "Understanding protein stability requires characterization of structural determinants of the folded and unfolded states. Many proteins are capable of populating partially folded states under specific solution conditions. Occasionally, coexistence of the folded and an unfolded state under non- or mildly denaturing conditions can be observed by NMR, allowing us to structurally probe these states under identical conditions. Here we report on a destabilized mutant of the B1 domain of protein G (GB1) whose equilibrium unfolding was systematically investigated. Backbone amide residual dipolar couplings (RDCs), the tryptophan Nepsilon-H resonance and the amide nitrogen transverse relaxation rates (R2s) for varying pH values and different temperatures were measured. The backbone amide RDCs indicate that prior to complete unfolding, two melting hot spots are formed at the turn around T11, L12 and K13 and the N terminus of the helix at A24 and T25. The RDCs for the low pH, thermally unfolded state of GB1 are very small and do not indicate the presence of any native-like structure. Amide nitrogen transverse relaxation rates for GB1 in the folded state at different temperatures exhibit large contributions from exchange processes and the associated dynamics display considerable heterogeneity. Our data provide clear evidence for intermediate conformations and multi-state equilibrium un/folding for this GB1 variant." @default.
• W2009653722 created "2016-06-24" @default.
• W2009653722 creator A5004995359 @default.
• W2009653722 creator A5042363295 @default.
• W2009653722 creator A5074112300 @default.
• W2009653722 date "2004-01-01" @default.
• W2009653722 modified "2023-10-14" @default.
• W2009653722 title "Insights into Conformation and Dynamics of Protein GB1 During Folding and Unfolding by NMR" @default.
• W2009653722 cites W1487346929 @default.
• W2009653722 cites W1505900198 @default.
• W2009653722 cites W1512176015 @default.
• W2009653722 cites W1560586614 @default.
• W2009653722 cites W1593425281 @default.
• W2009653722 cites W1596502325 @default.
• W2009653722 cites W1969227831 @default.
• W2009653722 cites W1972459558 @default.
• W2009653722 cites W1980211602 @default.
• W2009653722 cites W2014095962 @default.
• W2009653722 cites W2020716464 @default.
• W2009653722 cites W2021641921 @default.
• W2009653722 cites W2021974163 @default.
• W2009653722 cites W2042961623 @default.
• W2009653722 cites W2048268480 @default.
• W2009653722 cites W2050622376 @default.
• W2009653722 cites W2058732302 @default.
• W2009653722 cites W2062557777 @default.
• W2009653722 cites W2067798279 @default.
• W2009653722 cites W2067989274 @default.
• W2009653722 cites W2073486807 @default.
• W2009653722 cites W2080837519 @default.
• W2009653722 cites W2082929083 @default.
• W2009653722 cites W2090189932 @default.
• W2009653722 cites W2094795801 @default.
• W2009653722 cites W2106390970 @default.
• W2009653722 cites W2121861656 @default.
• W2009653722 cites W2129879061 @default.
• W2009653722 cites W2131823126 @default.
• W2009653722 cites W2156995522 @default.
• W2009653722 cites W2165768701 @default.
• W2009653722 cites W2169821755 @default.
• W2009653722 doi "https://doi.org/10.1016/j.jmb.2003.11.042" @default.
• W2009653722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14729345" @default.
• W2009653722 hasPublicationYear "2004" @default.
• W2009653722 type Work @default.
• W2009653722 sameAs 2009653722 @default.
• W2009653722 citedByCount "76" @default.
• W2009653722 countsByYear W20096537222012 @default.
• W2009653722 countsByYear W20096537222013 @default.
• W2009653722 countsByYear W20096537222014 @default.
• W2009653722 countsByYear W20096537222015 @default.
• W2009653722 countsByYear W20096537222016 @default.
• W2009653722 countsByYear W20096537222017 @default.
• W2009653722 countsByYear W20096537222018 @default.
• W2009653722 countsByYear W20096537222019 @default.
• W2009653722 countsByYear W20096537222020 @default.
• W2009653722 countsByYear W20096537222021 @default.
• W2009653722 countsByYear W20096537222022 @default.
• W2009653722 crossrefType "journal-article" @default.
• W2009653722 hasAuthorship W2009653722A5004995359 @default.
• W2009653722 hasAuthorship W2009653722A5042363295 @default.
• W2009653722 hasAuthorship W2009653722A5074112300 @default.
• W2009653722 hasConcept C119599485 @default.
• W2009653722 hasConcept C127413603 @default.
• W2009653722 hasConcept C139447449 @default.
• W2009653722 hasConcept C158617107 @default.
• W2009653722 hasConcept C162203774 @default.
• W2009653722 hasConcept C169760540 @default.
• W2009653722 hasConcept C185592680 @default.
• W2009653722 hasConcept C19472624 @default.
• W2009653722 hasConcept C204328495 @default.
• W2009653722 hasConcept C206276672 @default.
• W2009653722 hasConcept C2776029896 @default.
• W2009653722 hasConcept C2776545253 @default.
• W2009653722 hasConcept C2778439535 @default.
• W2009653722 hasConcept C47701112 @default.
• W2009653722 hasConcept C55493867 @default.
• W2009653722 hasConcept C66974803 @default.
• W2009653722 hasConcept C71240020 @default.
• W2009653722 hasConcept C8010536 @default.
• W2009653722 hasConcept C86803240 @default.
• W2009653722 hasConceptScore W2009653722C119599485 @default.
• W2009653722 hasConceptScore W2009653722C127413603 @default.
• W2009653722 hasConceptScore W2009653722C139447449 @default.
• W2009653722 hasConceptScore W2009653722C158617107 @default.
• W2009653722 hasConceptScore W2009653722C162203774 @default.
• W2009653722 hasConceptScore W2009653722C169760540 @default.
• W2009653722 hasConceptScore W2009653722C185592680 @default.
• W2009653722 hasConceptScore W2009653722C19472624 @default.
• W2009653722 hasConceptScore W2009653722C204328495 @default.
• W2009653722 hasConceptScore W2009653722C206276672 @default.
• W2009653722 hasConceptScore W2009653722C2776029896 @default.
• W2009653722 hasConceptScore W2009653722C2776545253 @default.
• W2009653722 hasConceptScore W2009653722C2778439535 @default.
• W2009653722 hasConceptScore W2009653722C47701112 @default.
• W2009653722 hasConceptScore W2009653722C55493867 @default.
• W2009653722 hasConceptScore W2009653722C66974803 @default.
• W2009653722 hasConceptScore W2009653722C71240020 @default.
• W2009653722 hasConceptScore W2009653722C8010536 @default.

• next