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- W2009698093 abstract "Abstract The high toxicity of clostridial neurotoxins primarily results from their specific binding and uptake into neurons. At motor neurons, the seven botulinum neurotoxin serotypes A–G (BoNT/A–G) inhibit acetylcholine release, leading to flaccid paralysis, while tetanus neurotoxin blocks neurotransmitter release in inhibitory neurons, resulting in spastic paralysis. Uptake of BoNT/A, B, E and G requires a dual interaction with gangliosides and the synaptic vesicle (SV) proteins synaptotagmin or SV2, whereas little is known about the entry mechanisms of the remaining serotypes. Here, we demonstrate that BoNT/F as wells depends on the presence of gangliosides, by employing phrenic nerve hemidiaphragm preparations derived from mice expressing GM3, GM2, GM1 and GD1a or only GM3. Subsequent site‐directed mutagenesis based on homology models identified the ganglioside binding site at a conserved location in BoNT/E and F. Using the mice phrenic nerve hemidiaphragm assay as a physiological model system, cross‐competition of full‐length neurotoxin binding by recombinant binding fragments, plus accelerated neurotoxin uptake upon increased electrical stimulation, indicate that BoNT/F employs SV2 as protein receptor, whereas BoNT/C and D utilise different SV receptor structures. The co‐precipitation of SV2A, B and C from Triton‐solubilised SVs by BoNT/F underlines this conclusion." @default.
- W2009698093 created "2016-06-24" @default.
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- W2009698093 date "2009-09-01" @default.
- W2009698093 modified "2023-09-30" @default.
- W2009698093 title "Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor" @default.
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- W2009698093 cites W18661633 @default.
- W2009698093 cites W1905643418 @default.
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- W2009698093 cites W1975357978 @default.
- W2009698093 cites W1976283234 @default.
- W2009698093 cites W1977478068 @default.
- W2009698093 cites W1977539490 @default.
- W2009698093 cites W1984235249 @default.
- W2009698093 cites W1984337870 @default.
- W2009698093 cites W1992192055 @default.
- W2009698093 cites W1995000809 @default.
- W2009698093 cites W1995616760 @default.
- W2009698093 cites W2003381605 @default.
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- W2009698093 cites W2018363788 @default.
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- W2009698093 cites W2023816853 @default.
- W2009698093 cites W2025609921 @default.
- W2009698093 cites W2031154197 @default.
- W2009698093 cites W2033213836 @default.
- W2009698093 cites W2033701533 @default.
- W2009698093 cites W2036059965 @default.
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- W2009698093 cites W2050632267 @default.
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- W2009698093 cites W2072108954 @default.
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- W2009698093 doi "https://doi.org/10.1111/j.1471-4159.2009.06298.x" @default.
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