Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009698362> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2009698362 endingPage "2194" @default.
- W2009698362 startingPage "2186" @default.
- W2009698362 abstract "We have measured the equilibrium binding of dioleoylphosphatidylcholine vesicles (800-A diameter) containing various densities of incorporated palmitoyl-alpha-bungarotoxin (PBGT) to acetylcholine receptor (AchR) enriched microsac membranes. We have previously shown that these PBGT vesicles bind specifically to the microsacs mediated by direct interactions with the AchRs [Grant, S. W., Babbitt, B. P., West, L. K., & Huang, L. (1982) Biochemistry 21, 1274-1279]. The percent binding of liposomal lipid and associated PBGT to excess AchR sites, as well as the inhibition of binding by pretreatment of microsacs with excess alpha-bungarotoxin (alpha BGT), was strongly dependent upon the protein/lipid molar ratio of the vesicles. In addition, there existed a threshold level of approximately six PBGT molecules per vesicle at which the binding increased dramatically. The apparent association constant, KAapp, for lipid vesicle-microsac membrane binding increased approximately 4800-fold (from 3.95 X 10(4) to 1.90 X 10(8) M-1) due to an increase of 20-fold in the vesicle-associated PBGT surface density. Direct competition for binding to microsac membranes between vesicles with different PBGT/lipid molar ratios indicated that multivalent binders could easily replace binders of lower valency when receptor sites were limited. Measurement of the temperature dependence of the KAapp indicated that weak (low valency) and medium strength (intermediate valency) PBGT vesicle binders bound to microsacs in a fashion similar to the binding of alpha BGT and PBGT to detergent-solubilized AchRs. Strong PBGT vesicle binders (high valency) appear to bind by a somewhat different mechanism. All results are discussed in terms of the effects of ligand (PBGT) valency on the binding strength of vesicles to microsac membranes." @default.
- W2009698362 created "2016-06-24" @default.
- W2009698362 creator A5033300046 @default.
- W2009698362 creator A5031149794 @default.
- W2009698362 date "1985-04-01" @default.
- W2009698362 modified "2023-09-23" @default.
- W2009698362 title "Effects of valency on thermodynamic parameters of specific membrane interaction" @default.
- W2009698362 cites W1522090571 @default.
- W2009698362 cites W1974837457 @default.
- W2009698362 cites W1981147697 @default.
- W2009698362 cites W1982224775 @default.
- W2009698362 cites W1983649348 @default.
- W2009698362 cites W1986304929 @default.
- W2009698362 cites W1995780540 @default.
- W2009698362 cites W1999860614 @default.
- W2009698362 cites W2005224113 @default.
- W2009698362 cites W2028179170 @default.
- W2009698362 cites W2033669846 @default.
- W2009698362 cites W2037319070 @default.
- W2009698362 cites W2047922666 @default.
- W2009698362 cites W2065740758 @default.
- W2009698362 cites W2082503490 @default.
- W2009698362 cites W2083104119 @default.
- W2009698362 cites W2087581028 @default.
- W2009698362 cites W2185335233 @default.
- W2009698362 cites W330286904 @default.
- W2009698362 doi "https://doi.org/10.1021/bi00330a012" @default.
- W2009698362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3995009" @default.
- W2009698362 hasPublicationYear "1985" @default.
- W2009698362 type Work @default.
- W2009698362 sameAs 2009698362 @default.
- W2009698362 citedByCount "2" @default.
- W2009698362 crossrefType "journal-article" @default.
- W2009698362 hasAuthorship W2009698362A5031149794 @default.
- W2009698362 hasAuthorship W2009698362A5033300046 @default.
- W2009698362 hasConcept C107824862 @default.
- W2009698362 hasConcept C12554922 @default.
- W2009698362 hasConcept C130316041 @default.
- W2009698362 hasConcept C138885662 @default.
- W2009698362 hasConcept C139007053 @default.
- W2009698362 hasConcept C170493617 @default.
- W2009698362 hasConcept C185592680 @default.
- W2009698362 hasConcept C32786932 @default.
- W2009698362 hasConcept C41625074 @default.
- W2009698362 hasConcept C41895202 @default.
- W2009698362 hasConcept C43617362 @default.
- W2009698362 hasConcept C55493867 @default.
- W2009698362 hasConcept C71240020 @default.
- W2009698362 hasConcept C8010536 @default.
- W2009698362 hasConcept C86803240 @default.
- W2009698362 hasConceptScore W2009698362C107824862 @default.
- W2009698362 hasConceptScore W2009698362C12554922 @default.
- W2009698362 hasConceptScore W2009698362C130316041 @default.
- W2009698362 hasConceptScore W2009698362C138885662 @default.
- W2009698362 hasConceptScore W2009698362C139007053 @default.
- W2009698362 hasConceptScore W2009698362C170493617 @default.
- W2009698362 hasConceptScore W2009698362C185592680 @default.
- W2009698362 hasConceptScore W2009698362C32786932 @default.
- W2009698362 hasConceptScore W2009698362C41625074 @default.
- W2009698362 hasConceptScore W2009698362C41895202 @default.
- W2009698362 hasConceptScore W2009698362C43617362 @default.
- W2009698362 hasConceptScore W2009698362C55493867 @default.
- W2009698362 hasConceptScore W2009698362C71240020 @default.
- W2009698362 hasConceptScore W2009698362C8010536 @default.
- W2009698362 hasConceptScore W2009698362C86803240 @default.
- W2009698362 hasIssue "9" @default.
- W2009698362 hasLocation W20096983621 @default.
- W2009698362 hasLocation W20096983622 @default.
- W2009698362 hasOpenAccess W2009698362 @default.
- W2009698362 hasPrimaryLocation W20096983621 @default.
- W2009698362 hasRelatedWork W196724569 @default.
- W2009698362 hasRelatedWork W1972462452 @default.
- W2009698362 hasRelatedWork W2009698362 @default.
- W2009698362 hasRelatedWork W2099556015 @default.
- W2009698362 hasRelatedWork W2121971847 @default.
- W2009698362 hasRelatedWork W2407203842 @default.
- W2009698362 hasRelatedWork W2410306754 @default.
- W2009698362 hasRelatedWork W3208844945 @default.
- W2009698362 hasRelatedWork W4319265373 @default.
- W2009698362 hasRelatedWork W4323293795 @default.
- W2009698362 hasVolume "24" @default.
- W2009698362 isParatext "false" @default.
- W2009698362 isRetracted "false" @default.
- W2009698362 magId "2009698362" @default.
- W2009698362 workType "article" @default.