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• W2009733104 abstract "A method is described for evaluation of fat-soluble vitamin in human adipose tissue with the aim to obtain, accurately and within the shortest analysis time, a time-integrated measure of exposure to vitamins from the diet. Fat tissue was deproteinized with ethanol and extracted with n-hexane. Normal-phase HPLC was performed in a Lichrosorb Si60 column with a gradient of n-hexane-2-propanol at 1 ml/min. Detection was accomplished using a diode-array system (for retinol and beta-carotene) in series with a fluorescence detector (alpha-tocopherol). The method was validated and applied to human adipose tissue in a total of 140 subjects. The mean contents found were 0.43, 0.84, 240.3 microg/g for retinol, beta-carotene and alpha-tocopherol, respectively. The method is sensitive enough for detecting the compounds in 1.6 mg of adipose tissue considering the lowest concentration found." @default.
• W2009733104 created "2016-06-24" @default.
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• W2009733104 date "2001-11-01" @default.
• W2009733104 modified "2023-09-26" @default.
• W2009733104 title "Simultaneous determination of retinol, β-carotene and α-tocopherol in adipose tissue by high-performance liquid chromatography" @default.
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• W2009733104 doi "https://doi.org/10.1016/s0378-4347(01)00349-8" @default.
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