FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009749435> ?p ?o ?g. }

• W2009749435 endingPage "55" @default.
• W2009749435 startingPage "48" @default.
• W2009749435 abstract "Background.Recent studies suggest that interleukin-1β (IL-1β) stimulates the production of the acute phase protein complement component C3 in human intestinal epithelial cells. The transcription factor NF-κB activates different genes involved in the response to cytokines. It is not known if IL-1β-induced C3 production in the enterocyte is regulated by NF-κB. Materials and methods.Cultured Caco-2 cells, a human intestinal epithelial cell line, were treated with one of the NF-κB inhibitors, tosyl-lys-chloromethylketone (TLCK), genistein, or pyrrolidine dithiocarbamate (PDTC), or withN-acetyl-leu-leu-norleucinal (LLnL), a proteasome inhibitor known to block the degradation of Iκβ, the cytosolic inhibitor of NF-κB. Following this treatment, the Caco-2 cells were stimulated with IL-1β, and C3 levels in the culture medium were measured after 24 h by ELISA. C3 mRNA levels were determined after 4 h by Northern blot analysis. In other experiments, Caco-2 cells were transfected with a mutant IκBα in which serines 32 and 36 were substituted by alanine. This mutation prevents IkBα phosphorylation and subsequent NF-κB nuclear translocation. After transfection, the cells were stimulated with IL-1β, and C3 levels in the culture medium were measured after 24 h. Cytosolic IκBα was determined by Western blot analysis. Results.TLCK, genistein, and LLnL each inhibited IL-1β-induced C3 production in a dose-dependent fashion. These responses were associated with decreased C3 mRNA levels. In contrast, PDTC did not influence C3 production or C3 mRNA in the Caco-2 cells. Transfection of the Caco-2 cells with the Ser 32/36 mutant IkBα resulted in maintained IκBα levels and decreased IL-β-induced C3 production. Conclusions.IL-1β-stimulated C3 production in the enterocyte may be regulated by NF-κB." @default.
• W2009749435 created "2016-06-24" @default.
• W2009749435 creator A5013430395 @default.
• W2009749435 creator A5013889334 @default.
• W2009749435 creator A5025631700 @default.
• W2009749435 creator A5030789558 @default.
• W2009749435 creator A5058049602 @default.
• W2009749435 creator A5060077166 @default.
• W2009749435 creator A5073095140 @default.
• W2009749435 creator A5078491855 @default.
• W2009749435 date "1999-03-01" @default.
• W2009749435 modified "2023-09-26" @default.
• W2009749435 title "Complement Component C3 Production in IL-1β-Stimulated Human Intestinal Epithelial Cells Is Blocked by NF-κB Inhibitors and by Transfection with Ser 32/36 Mutant IκBα" @default.
• W2009749435 cites W114601441 @default.
• W2009749435 cites W1520976404 @default.
• W2009749435 cites W1535872730 @default.
• W2009749435 cites W1568529372 @default.
• W2009749435 cites W1656644300 @default.
• W2009749435 cites W1929098742 @default.
• W2009749435 cites W1967327478 @default.
• W2009749435 cites W1970821592 @default.
• W2009749435 cites W1971290197 @default.
• W2009749435 cites W1980166683 @default.
• W2009749435 cites W1980469685 @default.
• W2009749435 cites W1981921664 @default.
• W2009749435 cites W1983668987 @default.
• W2009749435 cites W1992889151 @default.
• W2009749435 cites W2008744347 @default.
• W2009749435 cites W2008994285 @default.
• W2009749435 cites W2055426473 @default.
• W2009749435 cites W2057093252 @default.
• W2009749435 cites W2075946395 @default.
• W2009749435 cites W2101759139 @default.
• W2009749435 cites W2105896882 @default.
• W2009749435 cites W2116650665 @default.
• W2009749435 cites W2127044259 @default.
• W2009749435 cites W2134812217 @default.
• W2009749435 cites W2142594309 @default.
• W2009749435 cites W2159567970 @default.
• W2009749435 cites W2161435735 @default.
• W2009749435 cites W2207929855 @default.
• W2009749435 cites W2238844610 @default.
• W2009749435 cites W2415566676 @default.
• W2009749435 cites W8670133 @default.
• W2009749435 cites W95988660 @default.
• W2009749435 doi "https://doi.org/10.1006/jsre.1998.5503" @default.
• W2009749435 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10068525" @default.
• W2009749435 hasPublicationYear "1999" @default.
• W2009749435 type Work @default.
• W2009749435 sameAs 2009749435 @default.
• W2009749435 citedByCount "46" @default.
• W2009749435 countsByYear W20097494352012 @default.
• W2009749435 countsByYear W20097494352013 @default.
• W2009749435 countsByYear W20097494352014 @default.
• W2009749435 countsByYear W20097494352015 @default.
• W2009749435 countsByYear W20097494352016 @default.
• W2009749435 countsByYear W20097494352017 @default.
• W2009749435 countsByYear W20097494352018 @default.
• W2009749435 countsByYear W20097494352019 @default.
• W2009749435 countsByYear W20097494352021 @default.
• W2009749435 countsByYear W20097494352022 @default.
• W2009749435 countsByYear W20097494352023 @default.
• W2009749435 crossrefType "journal-article" @default.
• W2009749435 hasAuthorship W2009749435A5013430395 @default.
• W2009749435 hasAuthorship W2009749435A5013889334 @default.
• W2009749435 hasAuthorship W2009749435A5025631700 @default.
• W2009749435 hasAuthorship W2009749435A5030789558 @default.
• W2009749435 hasAuthorship W2009749435A5058049602 @default.
• W2009749435 hasAuthorship W2009749435A5060077166 @default.
• W2009749435 hasAuthorship W2009749435A5073095140 @default.
• W2009749435 hasAuthorship W2009749435A5078491855 @default.
• W2009749435 hasConcept C104317684 @default.
• W2009749435 hasConcept C105580179 @default.
• W2009749435 hasConcept C134018914 @default.
• W2009749435 hasConcept C153911025 @default.
• W2009749435 hasConcept C177707886 @default.
• W2009749435 hasConcept C185592680 @default.
• W2009749435 hasConcept C193176779 @default.
• W2009749435 hasConcept C2776415932 @default.
• W2009749435 hasConcept C2777730290 @default.
• W2009749435 hasConcept C2777882243 @default.
• W2009749435 hasConcept C2778298627 @default.
• W2009749435 hasConcept C2779604457 @default.
• W2009749435 hasConcept C2779730028 @default.
• W2009749435 hasConcept C2780983412 @default.
• W2009749435 hasConcept C54009773 @default.
• W2009749435 hasConcept C54355233 @default.
• W2009749435 hasConcept C55493867 @default.
• W2009749435 hasConcept C62478195 @default.
• W2009749435 hasConcept C81885089 @default.
• W2009749435 hasConcept C86803240 @default.
• W2009749435 hasConcept C95444343 @default.
• W2009749435 hasConceptScore W2009749435C104317684 @default.
• W2009749435 hasConceptScore W2009749435C105580179 @default.
• W2009749435 hasConceptScore W2009749435C134018914 @default.
• W2009749435 hasConceptScore W2009749435C153911025 @default.
• W2009749435 hasConceptScore W2009749435C177707886 @default.
• W2009749435 hasConceptScore W2009749435C185592680 @default.

• next