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- W2009794585 abstract "1. Biosynthesis of glycerides was measured using rat-liver mitochondria or the microsomal fraction of cat intestinal mucosa. With glycerolphosphate as precursor, the incorporation of radioactive palmitate into glycerides was stimulated up to 2.6-fold by the addition of 20 to 100 μM. unsaturated long-chain fatty acids which were themselves incorporated. At the same time, the amount of labelled phosphatidate present showed a corresponding decrease. 2. Out of 5 unsaturated long-chain fatty acids tested, linoleate and linolenate gave the largest stimulations, while saturated fatty acids were ineffective. Under optimum conditions, the molar ratio of palmitate to linoleate incorporated into glycerides was approx. 2.5. 3. The addition of particle-free supernatant to either the mitochondrial or microsomal system brought about a much larger stimulation of palmitate incorporation than did unsaturated fatty acids. Evidence is presented that the stimulating effect of the particle-free supernatant is partially due to the presence of unsaturated fatty acids in this fraction. 4. The formation of higher glycerides from 1-monopalmitin or from 1,2-diglyceride prepared from egg lecithin was not or only slightly stimulated by the addition of unsaturated fatty acids or the particle-free supernatant." @default.
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- W2009794585 title "The effect of unsaturated fatty acids and the particle-free supernatant on the incorporation of palmitate into glycerides" @default.
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- W2009794585 doi "https://doi.org/10.1016/0005-2760(67)90158-0" @default.
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