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- W2009801114 abstract "Filarial nematode parasites cause serious diseases such as elephantiasis and river blindness in humans, and heartworm infections in dogs. Third stage filarial larvae (L3) are a critical stage in the life cycle of filarial parasites, because this is the stage that is transmitted by arthropod vectors to initiate infections in mammals. Improved understanding of molecular mechanisms associated with this transition may provide important leads for development of new therapies and vaccines to prevent filarial infections. This study explores changes in gene expression associated with the transition of Brugia malayi third stage larvae (BmL3) from mosquitoes into mammalian hosts and how these changes are affected by radiation. Radiation effects are especially interesting because irradiated L3 induce partial immunity to filarial infections. The underlying molecular mechanisms responsible for the efficacy of such vaccines are unkown.Expression profiles were obtained using a new filarial microarray with 18, 104 64-mer elements. 771 genes were identified as differentially expressed in two-way comparative analyses of the three L3 types. 353 genes were up-regulated in mosquito L3 (L3i) relative to cultured L3 (L3c). These genes are important for establishment of filarial infections in mammalian hosts. Other genes were up-regulated in L3c relative to L3i (234) or irradiated L3 (L3ir) (22). These culture-induced transcripts include key molecules required for growth and development. 165 genes were up-regulated in L3ir relative to L3c; these genes encode highly immunogenic proteins and proteins involved in radiation repair. L3ir and L3i have similar transcription profiles for genes that encode highly immunogenic proteins, antioxidants and cuticle components.Changes in gene expression that normally occur during culture under conditions that support L3 development and molting are prevented or delayed by radiation. This may explain the enhanced immunogenicity of L3ir. Gene Ontology and KEGG analyses revealed altered pathways between L3 types. Energy and immune pathways are up-regulated and may be needed for L3i invasion and survival, while growth and development are priorities for L3c. This study has improved our understanding of molecules involved in parasite invasion and immune evasion, potential targets of protective immunity, and molecules required for parasite growth and development." @default.
- W2009801114 created "2016-06-24" @default.
- W2009801114 creator A5028219535 @default.
- W2009801114 creator A5045568020 @default.
- W2009801114 creator A5068833971 @default.
- W2009801114 creator A5074369323 @default.
- W2009801114 creator A5078964229 @default.
- W2009801114 creator A5081639916 @default.
- W2009801114 creator A5085897306 @default.
- W2009801114 date "2009-01-01" @default.
- W2009801114 modified "2023-10-08" @default.
- W2009801114 title "Transcriptomes and pathways associated with infectivity, survival and immunogenicity in Brugia malayi L3" @default.
- W2009801114 cites W1490525966 @default.
- W2009801114 cites W1518457092 @default.
- W2009801114 cites W1537387721 @default.
- W2009801114 cites W1545357297 @default.
- W2009801114 cites W1606328556 @default.
- W2009801114 cites W1631908837 @default.
- W2009801114 cites W1882527013 @default.
- W2009801114 cites W1943367294 @default.
- W2009801114 cites W1949406643 @default.
- W2009801114 cites W1981415201 @default.
- W2009801114 cites W1986668201 @default.
- W2009801114 cites W1987325894 @default.
- W2009801114 cites W1997001730 @default.
- W2009801114 cites W1997484754 @default.
- W2009801114 cites W1997549876 @default.
- W2009801114 cites W1998653602 @default.
- W2009801114 cites W2000397358 @default.
- W2009801114 cites W2004117621 @default.
- W2009801114 cites W2004958759 @default.
- W2009801114 cites W2006234418 @default.
- W2009801114 cites W2009754050 @default.
- W2009801114 cites W2017846611 @default.
- W2009801114 cites W2017977879 @default.
- W2009801114 cites W2020903815 @default.
- W2009801114 cites W2022586388 @default.
- W2009801114 cites W2025576707 @default.
- W2009801114 cites W2026546941 @default.
- W2009801114 cites W2027872459 @default.
- W2009801114 cites W2030594651 @default.
- W2009801114 cites W2031942563 @default.
- W2009801114 cites W2038492783 @default.
- W2009801114 cites W2049636890 @default.
- W2009801114 cites W2053166490 @default.
- W2009801114 cites W2055043387 @default.
- W2009801114 cites W2055930797 @default.
- W2009801114 cites W2058538505 @default.
- W2009801114 cites W2065358746 @default.
- W2009801114 cites W2069162010 @default.
- W2009801114 cites W2069716005 @default.
- W2009801114 cites W2070267552 @default.
- W2009801114 cites W2072002687 @default.
- W2009801114 cites W2076397118 @default.
- W2009801114 cites W2077757534 @default.
- W2009801114 cites W2077896867 @default.
- W2009801114 cites W2080766530 @default.
- W2009801114 cites W2081032837 @default.
- W2009801114 cites W2087211784 @default.
- W2009801114 cites W2089432616 @default.
- W2009801114 cites W2090721573 @default.
- W2009801114 cites W2090870669 @default.
- W2009801114 cites W2091448229 @default.
- W2009801114 cites W2095668172 @default.
- W2009801114 cites W2097265502 @default.
- W2009801114 cites W2101595316 @default.
- W2009801114 cites W2103017472 @default.
- W2009801114 cites W2104184569 @default.
- W2009801114 cites W2104605579 @default.
- W2009801114 cites W2105106534 @default.
- W2009801114 cites W2105866364 @default.
- W2009801114 cites W2108819608 @default.
- W2009801114 cites W2109160513 @default.
- W2009801114 cites W2111853426 @default.
- W2009801114 cites W2114942606 @default.
- W2009801114 cites W2116070232 @default.
- W2009801114 cites W2117856189 @default.
- W2009801114 cites W2120319312 @default.
- W2009801114 cites W2127379971 @default.
- W2009801114 cites W2128491398 @default.
- W2009801114 cites W2131631319 @default.
- W2009801114 cites W2132285289 @default.
- W2009801114 cites W2133648770 @default.
- W2009801114 cites W2139062153 @default.
- W2009801114 cites W2143531281 @default.
- W2009801114 cites W2144008452 @default.
- W2009801114 cites W2147515706 @default.
- W2009801114 cites W2148917681 @default.
- W2009801114 cites W2151171010 @default.
- W2009801114 cites W2151334936 @default.
- W2009801114 cites W2154265462 @default.
- W2009801114 cites W2157772863 @default.
- W2009801114 cites W2157991230 @default.
- W2009801114 cites W2159482845 @default.
- W2009801114 cites W2162195838 @default.
- W2009801114 cites W2166054142 @default.
- W2009801114 cites W2167789038 @default.
- W2009801114 cites W2169804929 @default.