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- W2009894531 abstract "M. MAREKOVÁ, V. KMET' AND P. JAVORSKÝ. 1996. The transformation and subsequent regeneration of ruminal strain Streptococcus bovis AO24/85 protoplasts by plasmid DNA was studied. The best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. Optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. Addition of Ca2+ and Mg2+ ions (2.5 mmol l-1) to the transformation medium increased the proportion of regenerated cells. Transformation frequencies were 3 times 103 transformants per μg of pNZ12 and 2.4 times 102 per μg of pJK108, respectively." @default.
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- W2009894531 date "1996-02-01" @default.
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- W2009894531 title "Transformation of Streptococcus bovis protoplasts by plasmid DNA" @default.
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- W2009894531 doi "https://doi.org/10.1111/j.1472-765x.1996.tb01132.x" @default.
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