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- W2009898308 abstract "The aim of the work was to determine the susceptibility of non-cultured and cultured rabbit embryos at different stages of development to the adverse effects of vitrification. The experiment was carried out on non-cultured embryos at the 8- to 16-cell, morula and blastocyst stages, and on embryos collected at the 2-cell stage and cultured for 24, 48 or 72 h. The embryos were cultured in Menezo's B2 medium supplemented with 20% FCS (fetal calf serum), or were cocultured with oviductal epithelium cells. A mixture of 35% glycerol + 35% 1,2-propanediol in serum-supplemented, phosphatebuffered saline was used as a vitrification medium. The embryos were vitrified in glass ampoules or plastic straws. Dilution after rapid thawing was done in one step, in a 1 M sucrose solution. After vitrification of non-cultured embryos in glass ampoules, the survival rates of the 8- to 16-cell, morula and blastocyst stages were 55.9%, 88.4% and 83.1%, respectively. The survival rates of embryos derived from cultures of 2-cell embryos in Menezo's B2 medium for 24 h (8- to 16-cell stage), 48 h (morula stage) and 72 h (blastocyst stage) and then vitrified in glass ampoules were 46.7%, 62.4% and 36.2%, respectively. The percentage of live foetuses remaining 28 days after the transfer of 103 vitrified and 115 cultured and vitrified 8- to 16-cell embryos was 21.4% and 1.8%, respectively. After the transfer of 111 vitrified morulae and 112 cultured and vitrified morulae, the percentage of live foetuses 28 days later was 11.7% and 7.1%, respectively. After vitrification in plastic straws, the survival rates in vitro of non-cultured morulae, and of morulae resulting from the culture of 2-cell embryos for 48 h in Menezo's B2 medium and in the coculture system were 89.1%, 93.8% and 91.2%, respectively." @default.
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- W2009898308 date "1991-11-01" @default.
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- W2009898308 title "Vitrification of non-cultured and cultured rabbit embryos" @default.
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- W2009898308 doi "https://doi.org/10.1016/0378-4320(91)90073-9" @default.
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