FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009968900> ?p ?o ?g. }

• W2009968900 endingPage "134" @default.
• W2009968900 startingPage "126" @default.
• W2009968900 abstract "Adeno-associated viral (AAV) vectors hold great potential for liver-directed gene therapy. Stable and high levels of transgene expression have been achieved in many murine models. Systemic delivery of AAV vectors in nonhuman primates (NHPs) that are natural hosts of AAVs appear to be challenging due to the high prevalence of pre-existing neutralizing antibodies (NAbs). This study evaluates the performance of AAV8, hu.37, and rh.8 vectors expressing green fluorescent protein (GFP) from a liver-specific promoter in rhesus macaques. Two of the animals that received AAV8 showed transduction of 24 and 40% of hepatocytes 7 days after systemic vector delivery. Importantly, expression was detected in several animals after 35 days despite the elevation of liver enzymes and development of transgene-specific T cells in liver. Pre-existing low levels of NAbs profoundly impacted the outcome of gene transfer and redirected vector DNA to spleen. We developed a sensitive in vivo passive transfer assay to detect low levels of NAbs to these novel AAV serotypes. Other strategies need to be developed to reduce immune response to the transgene in order to maintain long-term gene expression. Adeno-associated viral (AAV) vectors hold great potential for liver-directed gene therapy. Stable and high levels of transgene expression have been achieved in many murine models. Systemic delivery of AAV vectors in nonhuman primates (NHPs) that are natural hosts of AAVs appear to be challenging due to the high prevalence of pre-existing neutralizing antibodies (NAbs). This study evaluates the performance of AAV8, hu.37, and rh.8 vectors expressing green fluorescent protein (GFP) from a liver-specific promoter in rhesus macaques. Two of the animals that received AAV8 showed transduction of 24 and 40% of hepatocytes 7 days after systemic vector delivery. Importantly, expression was detected in several animals after 35 days despite the elevation of liver enzymes and development of transgene-specific T cells in liver. Pre-existing low levels of NAbs profoundly impacted the outcome of gene transfer and redirected vector DNA to spleen. We developed a sensitive in vivo passive transfer assay to detect low levels of NAbs to these novel AAV serotypes. Other strategies need to be developed to reduce immune response to the transgene in order to maintain long-term gene expression." @default.
• W2009968900 created "2016-06-24" @default.
• W2009968900 creator A5009839456 @default.
• W2009968900 creator A5023492006 @default.
• W2009968900 creator A5031060856 @default.
• W2009968900 creator A5036549445 @default.
• W2009968900 creator A5046895078 @default.
• W2009968900 creator A5046993622 @default.
• W2009968900 creator A5051344823 @default.
• W2009968900 creator A5073189210 @default.
• W2009968900 creator A5080379416 @default.
• W2009968900 creator A5083739731 @default.
• W2009968900 date "2010-01-01" @default.
• W2009968900 modified "2023-10-17" @default.
• W2009968900 title "The Pleiotropic Effects of Natural AAV Infections on Liver-directed Gene Transfer in Macaques" @default.
• W2009968900 cites W1577558588 @default.
• W2009968900 cites W1971410862 @default.
• W2009968900 cites W1976329054 @default.
• W2009968900 cites W1997005237 @default.
• W2009968900 cites W2000185853 @default.
• W2009968900 cites W2007261697 @default.
• W2009968900 cites W2020307339 @default.
• W2009968900 cites W2023133801 @default.
• W2009968900 cites W2027903919 @default.
• W2009968900 cites W2044474105 @default.
• W2009968900 cites W2045292147 @default.
• W2009968900 cites W2045817726 @default.
• W2009968900 cites W2047608515 @default.
• W2009968900 cites W2047667011 @default.
• W2009968900 cites W2049592320 @default.
• W2009968900 cites W2057548071 @default.
• W2009968900 cites W2060405418 @default.
• W2009968900 cites W2067204822 @default.
• W2009968900 cites W2075638876 @default.
• W2009968900 cites W2077387683 @default.
• W2009968900 cites W2080951809 @default.
• W2009968900 cites W2082549237 @default.
• W2009968900 cites W2091177028 @default.
• W2009968900 cites W2100371398 @default.
• W2009968900 cites W2111837903 @default.
• W2009968900 cites W2119053160 @default.
• W2009968900 cites W2128033750 @default.
• W2009968900 cites W2131862356 @default.
• W2009968900 cites W2136085939 @default.
• W2009968900 cites W2137222303 @default.
• W2009968900 cites W2141575938 @default.
• W2009968900 cites W2148848239 @default.
• W2009968900 cites W2151219050 @default.
• W2009968900 cites W2151809142 @default.
• W2009968900 cites W2158522626 @default.
• W2009968900 cites W2158749858 @default.
• W2009968900 cites W2170205772 @default.
• W2009968900 cites W2171985637 @default.
• W2009968900 cites W2174994066 @default.
• W2009968900 doi "https://doi.org/10.1038/mt.2009.245" @default.
• W2009968900 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2839221" @default.
• W2009968900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19888196" @default.
• W2009968900 hasPublicationYear "2010" @default.
• W2009968900 type Work @default.
• W2009968900 sameAs 2009968900 @default.
• W2009968900 citedByCount "115" @default.
• W2009968900 countsByYear W20099689002012 @default.
• W2009968900 countsByYear W20099689002013 @default.
• W2009968900 countsByYear W20099689002014 @default.
• W2009968900 countsByYear W20099689002015 @default.
• W2009968900 countsByYear W20099689002016 @default.
• W2009968900 countsByYear W20099689002017 @default.
• W2009968900 countsByYear W20099689002018 @default.
• W2009968900 countsByYear W20099689002019 @default.
• W2009968900 countsByYear W20099689002020 @default.
• W2009968900 countsByYear W20099689002021 @default.
• W2009968900 countsByYear W20099689002022 @default.
• W2009968900 countsByYear W20099689002023 @default.
• W2009968900 crossrefType "journal-article" @default.
• W2009968900 hasAuthorship W2009968900A5009839456 @default.
• W2009968900 hasAuthorship W2009968900A5023492006 @default.
• W2009968900 hasAuthorship W2009968900A5031060856 @default.
• W2009968900 hasAuthorship W2009968900A5036549445 @default.
• W2009968900 hasAuthorship W2009968900A5046895078 @default.
• W2009968900 hasAuthorship W2009968900A5046993622 @default.
• W2009968900 hasAuthorship W2009968900A5051344823 @default.
• W2009968900 hasAuthorship W2009968900A5073189210 @default.
• W2009968900 hasAuthorship W2009968900A5080379416 @default.
• W2009968900 hasAuthorship W2009968900A5083739731 @default.
• W2009968900 hasBestOaLocation W20099689001 @default.
• W2009968900 hasConcept C102230213 @default.
• W2009968900 hasConcept C104317684 @default.
• W2009968900 hasConcept C111599444 @default.
• W2009968900 hasConcept C135983454 @default.
• W2009968900 hasConcept C15152581 @default.
• W2009968900 hasConcept C159047783 @default.
• W2009968900 hasConcept C159654299 @default.
• W2009968900 hasConcept C203014093 @default.
• W2009968900 hasConcept C207001950 @default.
• W2009968900 hasConcept C2522874641 @default.
• W2009968900 hasConcept C2778107364 @default.
• W2009968900 hasConcept C2779261636 @default.
• W2009968900 hasConcept C2780931953 @default.

• next