FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010013311> ?p ?o ?g. }

• W2010013311 endingPage "522" @default.
• W2010013311 startingPage "512" @default.
• W2010013311 abstract "Previous studies with soluble enzyme preparations from sage (Salvia officinalis) demonstrated that the monoterpene ketone (+)-camphor was synthesized by the cyclization of neryl pyrophosphate to (+)-bornyl pyrophosphate followed by hydrolysis of this unusual intermediate to (+)-borneol and then oxidation of the alcohol to camphor (R. Croteau, and F. Karp, 1977, Arch. Biochem. Biophys.184, 77–86). Preliminary investigation of the (+)-bornyl pyrophosphate synthetase in crude preparations indicated that both neryl pyrophosphate and geranyl pyrophosphate could be cyclized to (+)-bornyl pyrophosphate, but the presence of high levels of phosphatases in the extract prevented an accurate assessment of substrate specificity. The competing phosphatases were removed by combination of gel filtration on Sephadex G-150, chromatography on hydroxylapatite, and chromatography on O-(diethylaminoethyl)-cellulose. In these fractionation steps, activities for the cyclization of neryl pyrophosphate and geranyl pyrophosphate to bornyl pyrophosphate were coincident, and on the removal of competing phosphatases, the synthetase was shown to prefer geranyl pyrophosphate as substrate (VKm for geranyl pyrophosphate was 20-fold that of neryl pyrophosphate). No interconversion of geranyl and neryl pyrophosphates was detected. The partially purified bornyl pyrophosphate synthetase had an apparent molecular weight of 95,000, and required Mg2+ for catalytic activity (Km for Mg2+ ~ 3.5 mm). Mn2+ and other divalent cations were ineffective in promoting the formation of bornyl pyrophosphate. The enzyme exhibited a pH optimum at 6.2 and was strongly inhibited by both p-hydroxymercuribenzoate and diisopropylfluorophosphate. Bornyl pyrophosphate synthetase is the first monoterpene synthetase to be isolated free from competing phosphatases, and the first to show a strong preference for geranyl pyrophosphate as substrate. A mechanism for the cyclization of geranyl pyrophosphate to bornyl pyrophosphate is proposed." @default.
• W2010013311 created "2016-06-24" @default.
• W2010013311 creator A5060790939 @default.
• W2010013311 creator A5083740089 @default.
• W2010013311 date "1979-12-01" @default.
• W2010013311 modified "2023-09-27" @default.
• W2010013311 title "Biosynthesis of monoterpenes: Preliminary characterization of bornyl pyrophosphate synthetase from sage (Salvia officinalis) and demonstration that geranyl pyrophosphate is the preferred substrate for cyclization" @default.
• W2010013311 cites W1572319845 @default.
• W2010013311 cites W1775749144 @default.
• W2010013311 cites W1977194678 @default.
• W2010013311 cites W1982528296 @default.
• W2010013311 cites W1988586129 @default.
• W2010013311 cites W2004794602 @default.
• W2010013311 cites W2007928253 @default.
• W2010013311 cites W2013507633 @default.
• W2010013311 cites W2029323885 @default.
• W2010013311 cites W2049344600 @default.
• W2010013311 cites W2050626627 @default.
• W2010013311 cites W2055969398 @default.
• W2010013311 cites W2062634740 @default.
• W2010013311 cites W2071103147 @default.
• W2010013311 cites W2074391120 @default.
• W2010013311 cites W2077249986 @default.
• W2010013311 cites W2077541178 @default.
• W2010013311 cites W2085033039 @default.
• W2010013311 cites W2091178344 @default.
• W2010013311 cites W2092924349 @default.
• W2010013311 cites W2093789334 @default.
• W2010013311 cites W2104545907 @default.
• W2010013311 cites W2156279741 @default.
• W2010013311 cites W2157983506 @default.
• W2010013311 cites W2196547254 @default.
• W2010013311 cites W3005342873 @default.
• W2010013311 doi "https://doi.org/10.1016/0003-9861(79)90526-5" @default.
• W2010013311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/42356" @default.
• W2010013311 hasPublicationYear "1979" @default.
• W2010013311 type Work @default.
• W2010013311 sameAs 2010013311 @default.
• W2010013311 citedByCount "96" @default.
• W2010013311 countsByYear W20100133112012 @default.
• W2010013311 countsByYear W20100133112013 @default.
• W2010013311 countsByYear W20100133112015 @default.
• W2010013311 countsByYear W20100133112017 @default.
• W2010013311 countsByYear W20100133112018 @default.
• W2010013311 countsByYear W20100133112020 @default.
• W2010013311 countsByYear W20100133112021 @default.
• W2010013311 countsByYear W20100133112022 @default.
• W2010013311 countsByYear W20100133112023 @default.
• W2010013311 crossrefType "journal-article" @default.
• W2010013311 hasAuthorship W2010013311A5060790939 @default.
• W2010013311 hasAuthorship W2010013311A5083740089 @default.
• W2010013311 hasConcept C111368507 @default.
• W2010013311 hasConcept C127313418 @default.
• W2010013311 hasConcept C134651460 @default.
• W2010013311 hasConcept C138885662 @default.
• W2010013311 hasConcept C178790620 @default.
• W2010013311 hasConcept C181199279 @default.
• W2010013311 hasConcept C185592680 @default.
• W2010013311 hasConcept C2776043855 @default.
• W2010013311 hasConcept C2776990775 @default.
• W2010013311 hasConcept C2777289219 @default.
• W2010013311 hasConcept C2777671706 @default.
• W2010013311 hasConcept C2778827360 @default.
• W2010013311 hasConcept C2780886089 @default.
• W2010013311 hasConcept C2911011617 @default.
• W2010013311 hasConcept C41895202 @default.
• W2010013311 hasConcept C553450214 @default.
• W2010013311 hasConcept C71240020 @default.
• W2010013311 hasConcept C94412978 @default.
• W2010013311 hasConceptScore W2010013311C111368507 @default.
• W2010013311 hasConceptScore W2010013311C127313418 @default.
• W2010013311 hasConceptScore W2010013311C134651460 @default.
• W2010013311 hasConceptScore W2010013311C138885662 @default.
• W2010013311 hasConceptScore W2010013311C178790620 @default.
• W2010013311 hasConceptScore W2010013311C181199279 @default.
• W2010013311 hasConceptScore W2010013311C185592680 @default.
• W2010013311 hasConceptScore W2010013311C2776043855 @default.
• W2010013311 hasConceptScore W2010013311C2776990775 @default.
• W2010013311 hasConceptScore W2010013311C2777289219 @default.
• W2010013311 hasConceptScore W2010013311C2777671706 @default.
• W2010013311 hasConceptScore W2010013311C2778827360 @default.
• W2010013311 hasConceptScore W2010013311C2780886089 @default.
• W2010013311 hasConceptScore W2010013311C2911011617 @default.
• W2010013311 hasConceptScore W2010013311C41895202 @default.
• W2010013311 hasConceptScore W2010013311C553450214 @default.
• W2010013311 hasConceptScore W2010013311C71240020 @default.
• W2010013311 hasConceptScore W2010013311C94412978 @default.
• W2010013311 hasIssue "2" @default.
• W2010013311 hasLocation W20100133111 @default.
• W2010013311 hasLocation W20100133112 @default.
• W2010013311 hasOpenAccess W2010013311 @default.
• W2010013311 hasPrimaryLocation W20100133111 @default.
• W2010013311 hasRelatedWork W1966592540 @default.
• W2010013311 hasRelatedWork W2010013311 @default.
• W2010013311 hasRelatedWork W2018120842 @default.
• W2010013311 hasRelatedWork W2042959160 @default.
• W2010013311 hasRelatedWork W2043464867 @default.
• W2010013311 hasRelatedWork W2055959936 @default.

• next