Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010015369> ?p ?o ?g. }
- W2010015369 endingPage "11075" @default.
- W2010015369 startingPage "11067" @default.
- W2010015369 abstract "G-protein-coupled receptors (GPCRs) play key roles in cellular signal transduction and many are pharmacologically important targets for drug discovery. GPCRs can be reconstituted in planar supported lipid bilayers (PSLBs) with retention of activity, which has led to development of GPCR-based biosensors and biochips. However, PSLBs composed of natural lipids lack the high stability desired for many technological applications. One strategy is to use synthetic lipid monomers that can be polymerized to form robust bilayers. A key question is how lipid polymerization affects GPCR structure and activity. Here we have investigated the photochemical activity of bovine rhodopsin (Rho), a model GPCR, reconstituted into PSLBs composed of lipids having one or two polymerizable dienoyl moieties located in different regions of the acyl chains. Plasmon waveguide resonance spectroscopy was used to compare the degree of Rho photoactivation in fluid and poly(lipid) PSLBs. The position of the dienoyl moiety was found to have a significant effect: polymerization near the glycerol backbone significantly attenuates Rho activity whereas polymerization near the acyl chain termini does not. Differences in cross-link density near the acyl chain termini also do not affect Rho activity. In unpolymerized PSLBs, an equimolar mixture of phosphatidylethanolamine and phosphatidylcholine (PC) lipids enhances activity relative to pure PC; however after polymerization, the enhancement is eliminated which is attributed to stabilization of the membrane lamellar phase. These results should provide guidance for the design of robust lipid bilayers functionalized with transmembrane proteins for use in membrane-based biochips and biosensors." @default.
- W2010015369 created "2016-06-24" @default.
- W2010015369 creator A5005781724 @default.
- W2010015369 creator A5006802932 @default.
- W2010015369 creator A5058374788 @default.
- W2010015369 creator A5061194946 @default.
- W2010015369 creator A5065128552 @default.
- W2010015369 creator A5082464687 @default.
- W2010015369 date "2008-08-30" @default.
- W2010015369 modified "2023-09-27" @default.
- W2010015369 title "Reconstitution of Rhodopsin into Polymerizable Planar Supported Lipid Bilayers: Influence of Dienoyl Monomer Structure on Photoactivation" @default.
- W2010015369 cites W1540205595 @default.
- W2010015369 cites W1825353903 @default.
- W2010015369 cites W1966995488 @default.
- W2010015369 cites W1969303095 @default.
- W2010015369 cites W1971426414 @default.
- W2010015369 cites W1972859890 @default.
- W2010015369 cites W1980017545 @default.
- W2010015369 cites W1983374973 @default.
- W2010015369 cites W1985386007 @default.
- W2010015369 cites W1986402769 @default.
- W2010015369 cites W1988611050 @default.
- W2010015369 cites W1989171306 @default.
- W2010015369 cites W1989675515 @default.
- W2010015369 cites W1997277462 @default.
- W2010015369 cites W1997553987 @default.
- W2010015369 cites W1998714288 @default.
- W2010015369 cites W1999316866 @default.
- W2010015369 cites W2000130623 @default.
- W2010015369 cites W2000238162 @default.
- W2010015369 cites W2002450452 @default.
- W2010015369 cites W2004586929 @default.
- W2010015369 cites W2005285326 @default.
- W2010015369 cites W2007742449 @default.
- W2010015369 cites W2010358621 @default.
- W2010015369 cites W2012505746 @default.
- W2010015369 cites W2017003131 @default.
- W2010015369 cites W2018887727 @default.
- W2010015369 cites W2018961952 @default.
- W2010015369 cites W2019580775 @default.
- W2010015369 cites W2021123040 @default.
- W2010015369 cites W2024378468 @default.
- W2010015369 cites W2024457375 @default.
- W2010015369 cites W2029015814 @default.
- W2010015369 cites W2034506702 @default.
- W2010015369 cites W2034936244 @default.
- W2010015369 cites W2036575757 @default.
- W2010015369 cites W2037664056 @default.
- W2010015369 cites W2038736781 @default.
- W2010015369 cites W2047120441 @default.
- W2010015369 cites W2048191546 @default.
- W2010015369 cites W2049007155 @default.
- W2010015369 cites W2051266336 @default.
- W2010015369 cites W2051401374 @default.
- W2010015369 cites W2051719425 @default.
- W2010015369 cites W2058360269 @default.
- W2010015369 cites W2061011256 @default.
- W2010015369 cites W2062803011 @default.
- W2010015369 cites W2064222684 @default.
- W2010015369 cites W2066654517 @default.
- W2010015369 cites W2066902197 @default.
- W2010015369 cites W2067451034 @default.
- W2010015369 cites W2072516696 @default.
- W2010015369 cites W2074490295 @default.
- W2010015369 cites W2075006900 @default.
- W2010015369 cites W2076111905 @default.
- W2010015369 cites W2076939184 @default.
- W2010015369 cites W2077822496 @default.
- W2010015369 cites W2084726980 @default.
- W2010015369 cites W2088849675 @default.
- W2010015369 cites W2091615898 @default.
- W2010015369 cites W2095569890 @default.
- W2010015369 cites W2097365951 @default.
- W2010015369 cites W2101934167 @default.
- W2010015369 cites W2103438589 @default.
- W2010015369 cites W2104477830 @default.
- W2010015369 cites W2112329754 @default.
- W2010015369 cites W2113183595 @default.
- W2010015369 cites W2120763581 @default.
- W2010015369 cites W2121568234 @default.
- W2010015369 cites W2124329888 @default.
- W2010015369 cites W2127504012 @default.
- W2010015369 cites W2130049582 @default.
- W2010015369 cites W2137588110 @default.
- W2010015369 cites W2139317066 @default.
- W2010015369 cites W2140704050 @default.
- W2010015369 cites W2141761648 @default.
- W2010015369 cites W2142298838 @default.
- W2010015369 cites W2142571776 @default.
- W2010015369 cites W2149963584 @default.
- W2010015369 cites W2150379629 @default.
- W2010015369 cites W2152339698 @default.
- W2010015369 cites W2161165713 @default.
- W2010015369 cites W2162504167 @default.
- W2010015369 cites W2171243379 @default.
- W2010015369 cites W2177086883 @default.
- W2010015369 cites W4241922471 @default.
- W2010015369 doi "https://doi.org/10.1021/la801835g" @default.