Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010022445> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2010022445 abstract "The effect of reverse transcriptase (RT) catalyzed mutations on continued extension of the nascent DNA chain was investigated. A system using the alpha-lac gene of beta-galactosidase as template and two sets of conditions was used. In one, RT was allowed to reassociate with the primer-template after falling off, while in a second RT was sequestered after dissociating. In the first condition, subsequent extension of errors that may have initially caused enzyme dissociation can occur. In the second, such errors would not be extended. Fully extended products were assayed by alpha-complementation to assess mutation frequency. A lower frequency in the latter scenario implies that some errors caused the polymerase to dissociate. Allowing only a single binding event lowered the mutation frequency of the products by about 1/2 suggesting that approximately 1 in 2 errors terminated synthesis. In other experiments, when added to a primer-template with a terminal mismatch at the 3' end, RT dissociated from the template about 50-90% of the time (depending on mismatch type) rather than extending. Running start reactions indicated that extension was more likely if an actively synthesizing RT made the mutation. RT RNase H cleavage analysis showed that 3' mismatches weakened the association of RT with the primer-terminus. Taken together, these results suggest that an actively synthesizing RT enzyme that has just made a mistake is likely bound in a configuration that generally enhances extension of the mistake. This is in contrast to RTs that must bind to then extend mismatches. The importance of these findings with respect to the mechanism of mutagenesis is discussed." @default.
- W2010022445 created "2016-06-24" @default.
- W2010022445 creator A5013289645 @default.
- W2010022445 creator A5060991437 @default.
- W2010022445 date "2003-04-22" @default.
- W2010022445 modified "2023-09-23" @default.
- W2010022445 title "Analysis of Mutations Made during Active Synthesis or Extension of Mismatched Substrates Further Define the Mechanism of HIV-RT Mutagenesis" @default.
- W2010022445 cites W1568159422 @default.
- W2010022445 cites W1581921347 @default.
- W2010022445 cites W1591988375 @default.
- W2010022445 cites W1675873109 @default.
- W2010022445 cites W1763313231 @default.
- W2010022445 cites W1982372125 @default.
- W2010022445 cites W2009571668 @default.
- W2010022445 cites W2020451593 @default.
- W2010022445 cites W2020528427 @default.
- W2010022445 cites W2063264168 @default.
- W2010022445 cites W2098697496 @default.
- W2010022445 cites W2117623876 @default.
- W2010022445 cites W2127753323 @default.
- W2010022445 cites W3119774889 @default.
- W2010022445 doi "https://doi.org/10.1021/bi026998n" @default.
- W2010022445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12741851" @default.
- W2010022445 hasPublicationYear "2003" @default.
- W2010022445 type Work @default.
- W2010022445 sameAs 2010022445 @default.
- W2010022445 citedByCount "6" @default.
- W2010022445 countsByYear W20100224452013 @default.
- W2010022445 countsByYear W20100224452014 @default.
- W2010022445 countsByYear W20100224452015 @default.
- W2010022445 countsByYear W20100224452016 @default.
- W2010022445 crossrefType "journal-article" @default.
- W2010022445 hasAuthorship W2010022445A5013289645 @default.
- W2010022445 hasAuthorship W2010022445A5060991437 @default.
- W2010022445 hasConcept C104317684 @default.
- W2010022445 hasConcept C143065580 @default.
- W2010022445 hasConcept C156719811 @default.
- W2010022445 hasConcept C16318435 @default.
- W2010022445 hasConcept C170748874 @default.
- W2010022445 hasConcept C178790620 @default.
- W2010022445 hasConcept C185592680 @default.
- W2010022445 hasConcept C188082640 @default.
- W2010022445 hasConcept C194857396 @default.
- W2010022445 hasConcept C2756471 @default.
- W2010022445 hasConcept C2777563447 @default.
- W2010022445 hasConcept C3017666073 @default.
- W2010022445 hasConcept C49105822 @default.
- W2010022445 hasConcept C501734568 @default.
- W2010022445 hasConcept C54355233 @default.
- W2010022445 hasConcept C552990157 @default.
- W2010022445 hasConcept C69766542 @default.
- W2010022445 hasConcept C82381507 @default.
- W2010022445 hasConcept C86803240 @default.
- W2010022445 hasConceptScore W2010022445C104317684 @default.
- W2010022445 hasConceptScore W2010022445C143065580 @default.
- W2010022445 hasConceptScore W2010022445C156719811 @default.
- W2010022445 hasConceptScore W2010022445C16318435 @default.
- W2010022445 hasConceptScore W2010022445C170748874 @default.
- W2010022445 hasConceptScore W2010022445C178790620 @default.
- W2010022445 hasConceptScore W2010022445C185592680 @default.
- W2010022445 hasConceptScore W2010022445C188082640 @default.
- W2010022445 hasConceptScore W2010022445C194857396 @default.
- W2010022445 hasConceptScore W2010022445C2756471 @default.
- W2010022445 hasConceptScore W2010022445C2777563447 @default.
- W2010022445 hasConceptScore W2010022445C3017666073 @default.
- W2010022445 hasConceptScore W2010022445C49105822 @default.
- W2010022445 hasConceptScore W2010022445C501734568 @default.
- W2010022445 hasConceptScore W2010022445C54355233 @default.
- W2010022445 hasConceptScore W2010022445C552990157 @default.
- W2010022445 hasConceptScore W2010022445C69766542 @default.
- W2010022445 hasConceptScore W2010022445C82381507 @default.
- W2010022445 hasConceptScore W2010022445C86803240 @default.
- W2010022445 hasLocation W20100224451 @default.
- W2010022445 hasLocation W20100224452 @default.
- W2010022445 hasOpenAccess W2010022445 @default.
- W2010022445 hasPrimaryLocation W20100224451 @default.
- W2010022445 hasRelatedWork W1534084062 @default.
- W2010022445 hasRelatedWork W1590050892 @default.
- W2010022445 hasRelatedWork W1659608558 @default.
- W2010022445 hasRelatedWork W1976439804 @default.
- W2010022445 hasRelatedWork W2006031828 @default.
- W2010022445 hasRelatedWork W2010022445 @default.
- W2010022445 hasRelatedWork W2056460200 @default.
- W2010022445 hasRelatedWork W2092428935 @default.
- W2010022445 hasRelatedWork W2153193209 @default.
- W2010022445 hasRelatedWork W2352493293 @default.
- W2010022445 isParatext "false" @default.
- W2010022445 isRetracted "false" @default.
- W2010022445 magId "2010022445" @default.
- W2010022445 workType "article" @default.